4.4. Direct determination of saliva proteins Protein contaminated with nucleic acids absorbed the light at wavelength 280 nm and it absorbs much strongly at wavelength 205 nm when it is free from nucleic acids. The UV-visible spectrophotometer was used in determination of saliva proteins (Figure 2.2). Cold trichloroacetic acid (10 % w/v ) was added to the sample‚ centrifuged for 10 minutes to precipitate protein. The absorbance of a known volume
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Purification of Green Fluorescent Protein Introduction: Transformation is used to introduce a gene coding for a foreign protein into bacteria. Hydrophobic Interaction Chromatography (HIC) is used to purify the foreign protein. Protein gel electrophoresis is used to check and analyze the pure protein. Research scientists use Green Fluorescent Protein (GFP) as a master or tag to learn about the biology of individual cells and multicultural organisms. This lab introduces a rapid method to purify
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It about modified starch Journal of Agricultural Science Vol. 2‚ No. 2‚ June 2010 requirements are properties such as viscosity‚ resistant to shear‚ low pH and high temperature. Accordingly‚ varieties of modified starches are used in food industry. Table 1 lists some of the modified starches that can be prepared from different sources to meet the marketing-related requirements. Today‚ modified food starch is a food additive and limits of its modification‚ use and labeling are clearly defined
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Treating Starch How are starch and cellulose treated to allow them to be used in yeast? Starches: · All potable alcohol and most fermentation industrial alcohol is currently made principally from grains. · Fermentation of starch from grain is somewhat more complex than fermentation of sugars because starch must first be converted to sugar and then to ethanol. · Starch is converted enzymatically to glucose either by diastase presents in sprouting grain or by fungal amylase. · The resulting
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The objective of this macromolecules lab was to identify the presence any of the major macromolecules in various every day food items. The three macromolecules that this lab was carried out for were carbohydrates‚ lipids‚ and proteins. There were five different experiments conducted and each of those experiments had one factor in common‚ they all had the same controls. The controls in this lab activity were already set for the lab activity. The controls were the distilled water and the baking soda
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Passages quoted from the text (with page numbers) Responses (interact with the text through analysis‚ predictions‚ evaluation‚ and connections‚ but don’t just summarize) 1. “On this count‚ most of the men in the room could rest easy. They had personal trainers‚ gym memberships‚ and enough nutritional awareness to avoid diets that were heavy in the foods they manufactured” (11). This just confirms a horrible truth: the food companies are very much aware of the lack of nutritional value in their
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Anatomy & Physiology Name ________________________________ Organic Molecules Review Multiple Choice Identify the letter of the choice that best completes the statement or answers the question. ____ 1. Ions are a. electrically neutral fragments of a molecule that has been split apart. b. polar molecules c. radioactive isotopes that give off alpha‚ beta‚ or gamma d. charged atoms or molecules that have gained or lost electrons. ____ 2. Sodium has eleven protons. How many electrons
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Laboratory Report The Plasma Membrane I. Introduction The Plasma membrane is the edge of life‚ the boundary that separates the cell from its surroundings. It controls the traffic of materials in and out of the cell. (Reece‚ 2011). It is incredibly thin that is very vital in maintaining the integrity of the cell. Not only does the plasma membrane bind the other organelles‚ it also forms a dynamic structure which gives them their remarkable activity and selectivity. (Hickman‚ 2008). Diffusion
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! EXERCISE 6! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! BIOLOGICALLY IMPORTANT MOLECULES! REPORT ! ! ! INTRODUCTION ! In this experiment‚ we will learn about biological important molecules by performing tests to detect the presence of carbohydrates‚ proteins‚ lipids‚ and nucleic acids. Also‚ we will use biochemical tests to identify the unknown compound that react in the following biochemical tests: Benedict’s test‚ Iodine test‚ Sudan IV test‚ Biuret’s
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government in children’s healthy eating habits is in marketing‚ advertisement‚ federal policies in pricing‚ and the regulation of food served in a child’s school cafeteria. I had never really focused on or considered this issue until after reading Salt‚ Sugar‚ Fat by Michael Moss. Who is to be held responsible for this obesity epidemic is something that has long since remained controversial‚ and is a topic I desire to write my final research paper on.
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