Mammalian Cell Culture Lab Manual Student guide of Cell Biology Laboratory Techniques 2012 Jay M. Feldman Cell Biology Professor Lagier 11/26/2012 Mammalian Cell Culture Lab Manual Student guide of Cell Biology Laboratory Techniques 2012 Jay M. Feldman Cell Biology Professor Lagier 11/26/2012 Table of Contents I. Introduction * About the author page 2 * Preface page 3 II. Laboratory Techniques * Cell Culture Technique page 4-5 * Sterile Technique
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One of the main learning experiences of this lab is to learn the importance of calibrating a sensor. In most cases‚ a sensor will not have an output that is exactly what you need. An example of this is a strain gage. A stain gage gives back a voltage‚ but with that given voltage a distance can be found. Introduction: In this experiment‚ the QNET-MECHKIT was used. This board has multiple compact sensors that can be used for experiments. For this lab we will be using the strain gage to find the
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PRE LAB REPORT Reynaldo Riboul TLC and Column Chromatography October 6‚ 2013 Table of Chemicals: Chemical Hazards Mol. Wt. Density Grams Moles Acetone Flammable‚ Irritant 58.08 g mol−1 0.791 g cm−3 2.0 g 0.0344 Hexane Flammable‚ Irritant‚ Dangerous to Environment 86.18 g mol−1 .6548 g mL−1 9.0 g 0.1044 Fluorene Very toxic to aquatic life with long lasting effects 166.223 g/mol 1.202 g/mL 0.3 g 0.00180 Fluorenone Irritant 180.20 g mol−1 1.13 g/cm3 0.3 g 0.00166
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LAB REPORT FOR EXPERIMENT 3 COPPER CYCLE OLANREWAJU OYINDAMOLA TUESDAY 27TH February 2013 Abstract This experiment is based on copper‚ to synthesize some copper compound using Copper (II) nitrate solution to obtain copper metal at the end. Changes of copper complexes when various are added and filtering out the precipitate by using Buchner funnel for vacuum filtration .The experiment started with preparation of copper (II) hydroxide and addition of copper oxide then addition of droplets
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Lab Report – Strong Example Problem/Question Does using a base coat before painting nails prevent chipping? Background Information When painting nails‚ some manicurists use a base coat before applying the polish. The purpose of a base coat is to help the polish stick to your nails more easily. Base coats also help to protect your natural nail from the staining effects of colored polishes. Ridge-filling base coats fill in the grooves and cracks in the nail to create a smoother appearance
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Lab 17 Amino Acids and Proteins Lab date 10/22/2013 12-1350 I Purpose The purpose of this experiment was to separate mixtures of II Method For a complete list of experimental procedures see prelab outline attachment #1. “For a complete list of experimental procedure see Seager‚ Spencer L. and Slabaugh‚ Michael R. Safety-Scale Laboratory Experiments for Chemistry for Today General‚ Organic and Biochemistry; Thomson Brooks/Cole‚ Belmont‚ CA‚ USA‚ 2008; pp.221-225”. III Data Part A Mass
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listed below. You must observe and analyze these as part of the lab. The Iced Tea Debate The Salty Soup The Fire Bug 2 Use the DATA chart provided for recording observation based on three demonstrations you will watch 3 Watch each part of the experimental demonstration and make preditions about wht will happen in each scenario . Record your preditions and observations in the Data an Observations section of your labatory report format. Problem: How can matter and energy be described in
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Respiration: How Cells Harvest Chemical Energy 1. A cell must work just to maintain its complex structure‚ for order is intrinsically unstable. Because of entropy (the second law of thermodynamics)‚ which states that any enclosed system tends toward disorganization‚ cells must constantly use energy to keep themselves in order. If the cell was to stop using energy and let itself go then it would eventually get so disorganized that it wouldn’t function properly. 2. With the help of enzymes‚ the cell systematically
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Bubble Inside a Bubble Materials • • • • • • • • Granulated sugar (we had our best results using Imperial Sugar and Dixie Crystals) Dish soap Water Tablespoon Scissors Pipette Cup Adult supervision Bubbles form because of a combination of water’s hydrogen bonds and the oily film you can see shimmer in the light. The oily film you see is actually two separate layers of soap attached to‚ and surrounding‚ hydrogen-bonded water. Solar Oven S’mores Materials • Pizza box • Two clear sheet protectors
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Abstract This lab’s purpose was to test the patterns of percolation in various places. This lab tested how soil would absorb water based on different places. Distance from a group of trees‚ distance from a swamp‚ and different types of soil were tested. From the results collected‚ you can not prove‚ but you can infer that the ability to absorb increases as you go farther away from a swamp‚ and a group of trees. The ability to absorb also increases based on how much silt is in soil. Introduction
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