Proteomics is the study of proteomes‚ which are groups of proteins in living systems. It is used to examine when and where proteins are being expressed‚ rate of degradation‚ modifications‚ how they interact with other proteins and more. The goal in this lab was to determine the similarities and differences in muscle proteins among 5 species of fish using SDS-PAGE and Western blotting. First‚ proteins are separated based on size. Second‚ antibodies are used to detect the protein of interest. Lastly‚ a substrate
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therefore be used to calculate the vitamin C content of a variety of fruit juices. Research Question – To determine how the content of Vitamin C (%) will differ in fresh fruits (orange‚ mango‚ lemon‚ lime‚ blackcurrant‚ pineapple) from that in processed fruit juices (orange‚ pineapple) by adding drops of the aforementioned fruit juices to DCPIP until the DCPIP gets decolorized. Variables Independent Variable – Type of fruit juice used (fresh or processed). NB: the juice from the fruits was freshly
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Name : Siti Zulaikha Binti Dahalan Matric. No : FIS 1307 3296 Name of Partner : Title of Experiment : Experiment 2‚ Uncertainty and Error Date Of Experiment : 1st October 2013 Course Name and Code : Physics Lab 1 (FP1021) Instructor’s name : Dr. Azah Nik Jaafar Objective 1. To measure Human Reaction Time 2. To find uncertainty and error of a measurement Apparatus 1. Ruler Theory Human Reaction Time is a measure of how quickly human can respond to a particular stimulus. This
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tubes 2) Boiling water bath 3) Microscope 4) Slides and cover slips 5) Pasteur pipettes MATERIALS: 1) pH test strips 2) Benedict’s reagent PROCEDURES: Collect the urine samples in a container. Make sure to not spill anywhere in the lab. Complete the tests and all results/ observation are in a proper table. (A) Observation of urine 1) The urine is sniffed and the description of the urine is described. Terminologies like slightly nutty‚ sweet/ fruity or pungent can be used
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Homeostasis Lab: The Effects of Exercise on Homeostasis Purpose: To discover the effect that various levels of exercise have on specific body parameters. Hypothesis: If a subject walks up and down a flight of stairs for eight minutes‚ then they start to sweat more‚ their heart rate‚ body temperature‚ and breathing rate will increase‚ and there will be a change in their skin color on their arms and face‚ because the body will be trying to maintain homeostasis. Materials: You will
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The purpose of this lab was to determine the effects of changes in variables such as temperature‚ pH and concentration on catalase enzyme activity. Proteins are organic compounds made up of amino acid subunits consisting of carboxyl and amino functional groups‚ linked together by peptide bonds through condensation reactions. They are an essential part of all living organisms. Enzymes are “biological catalyst used to speed up chemical reactions (Athanasopoulos‚ 2014). Normally‚ for chemical reactions;
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Electrostatics Lab Name: Bryan Michel Materials: Styrofoam cups Styrofoam plates PVC Pipe Fur Scotch Tape Procedures: You will be investigating charging object by friction. When you are asked to write your observations‚ please do so in a clear‚ complete manner. 1. Tape a piece of string to a Styrofoam cup and hang it from the edge of the desk so that it can move freely. Rub the outside of the cup with fur. Rub a PVC pipe with fur and bring it near the hanging cup.
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In fermentation the pyruvate is converted into ethyl alcohol‚ through the oxidation of the 2 NADH molecules‚ which returns them to two NAD+’s (Freeman‚ 2011). Oxidation is the loss of an electron in this case H+. We used information from previous labs in which we tested
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Enzymes are catalytic proteins that speed up chemical reactions by lowering the activation energy of the reaction. our body cells depend highly on enzymes to complete many important functions‚ such as digestion and metabolism. Enzymes are selective‚ in which each enzyme can only speed up a specific reaction. There are molecules that enzymes work with called substrates. substrate bind to an area of an enzyme called active site. There is specificity between the enzyme and substrate that react with
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Medical Sciences. Wiley‚ Chichester. APPENDIX- Safety Assessment 1. General Aspects Gloves must be worn at all times to protect from pigment staining. Safety glasses must also be worn throughout the lab to protect the eyes from chemicals. Potential injury may arise from razor blades and cork borers. Lab coats must be fully fastened to protect clothes from staining. 2. Chemical Hazards The solvents (acetone and methanol) should not be inhaled as it can cause damage to mucous membranes. They can also
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