Separation of Amino Acids by Cation Exchange Chromatography Introduction and Purpose: Amino acids are small biomolecules that have a carboxylic acid backbone in common‚ as well as an amino group attached to a saturated carbon. There are many amino acids‚ but there are 20 most commonly know amino acids. Amino acids are the fundamenta building blocks of other biomolecules like proteins and ezymes (Davidson‚ 2015). This experiment examined a mixture of 3 amino acids. The purpose of this experiment
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Separation and Qualitative Determination of Cations and Anions Instructor’s Guide Master Materials List (complete for 24 students working in pairs) Silver nitrate‚ AgNO3‚ 0.2 M‚ 100 mL* Silver nitrate‚ AgNO3‚ 0.1 M‚ 100 mL* Cupric nitrate‚ Cu(No3)2‚ .02 M‚ 100 mL* Zinc nitrate‚ Zn(NO3)2‚ 0.2 M‚ 100 mL* Ferric nitrate‚ Zn(NO3)2‚ 0.2 M‚ 100 mL* Hydrochloric acid‚ HCl‚ 6 M‚ 125 mL* Nitric acid‚ HNO3‚ 6 M‚ 125 mL* Ammonia‚ NH3‚ 6 M‚ 125 mL* Sulfuric acid‚ H2SO4‚ 6 M‚ 125 mL* Acetic acid
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Introduction The purpose of this experiment was to find the identity of the unknown compound #12. This was done both qualitatively and quantitatively. The qualitative observations were used to determine the functional group that was in the unknown‚ the quantitative results were used to determine the molar mass through Duma’s method. The types of compounds that were used in testing were all functional groups‚ Aldehyde‚ Ketone‚ Alcohol and‚ Ester. Functional groups are a portion of a molecule that
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Exercise 14: Qualitative Anion Tests PURPOSE: The purpose of this experiment is to identify some commonly occurring anions & to study some of the reactions used for their identification. PROCEDURE: You must first determine to which of the three groups the various anions belong. Since no two people observe the same event in exactly the same way it is important to also conduct specific confirmation tests on the known samples of each anion. This information will help identify the anion
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A flame test is conducted when an element is burned and the flame produces a color different the starting flame color. When the coffee stirrer was put into the flame‚ the element’s atoms (from the solution of the stirrer) left the original level of energy. When the atoms fell back to ground level‚ they released photons in the form of colored flame. Using the flame test‚ unknown compounds can be identified when they are burned since each element has a specific color. (Douma‚ 2008). The color
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Potentiometric Titration of Sodium Carbonate Otieno O. Victor University of Detroit Mercy Quantitative Analysis Lab CHM 3880 Fall 2011 Partner: Edwin Gay Abstract The PH at each point during the titration of sodium carbonate unknown sample was determined. An Unknown sample of Na2CO3 was titrated with a standard HCL solution. In addition to titration‚ the pH at each point of titration was measured using PH meter. The % of the unknown Na2CO3 was 25.83% Introduction1 The purpose of this experiment
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Bunsen and Gustav Kirchoff who used flames tests that led to the discovery of the elements rubidium (Rb) and caesium (Cs). After this‚ this method was used everywhere to find the chemical make ups of many substances. For example‚ in 2007 there was a huge problem where tainted pet food would cause many pets to become ill. Qualitative analysis was used and it was discovered that Melamine was found in the pet food which altered the results of the protein tests‚ in which caused more ammonia to be generated
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Write up for Qualitative Analysis 1. Introduction- This experiment is for qualitative analysis so we are trying to find out what is in a substance. In this case we are using the flame test to try and discover what is in 4 unknown sample solutions that we have been given this is done by cross referencing the colour of the flame with 6 known solutions we test beforehand. Aim- In this experiment we are attempting to distinguish what substances are in the 4 unknown solutions by cross referencing
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Qualitative Test for Some Cations I (The Silver Group) Objective: At the end of the class the student should be able to: 1. Separate‚ detect and identify the ions in a given unknown solution possibly containing the ions of the silver group. 2. Identify the substance responsible for the positive result of each test 3. Point out the suitable conditions necessary for the detection process and the reasons for such conditions Introduction Procedure: 1. 3 test tube labeled 1‚2‚3 was obtained
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QUALITATIVE TESTS OF CARBOHYDRATES Final Lab Report Submitted by Brittany Fitzgerald 545301 Prepared for Nancy Cook Chemistry 3501 Monday November 26‚ 2012 RESULTS PART A: As a result of the Benedict’s test on various sugar solutions‚ it was found that galactose‚ mannose‚ arabinose‚ ribose‚ lactose‚ fructose‚ maltose‚ and cellobiose tested positively and therefore are considered reducing sugars. Glucose‚ starch‚ sucrose‚ and methyl-D-glucopyranoside on the other hand tested
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