We labeled the test tubes with numbers one through five. The first test tube was our calibration tube‚ here we added one milliliter (mL) of buffer‚ four mL of distilled water and three drops of chloroplasts using pipets. After‚ we created the solution for the second‚ third‚ fourth and fifth test tubes by adding one mL of buffer‚ three mL of distilled water one mL of DPIP an 3 drops of chloroplasts. However‚ we decided that test tube number two was going to be the test tube that was kept completely
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is unlimited per the lab manual.(Bluedoor) If there is no competition in the water‚ the growth can be unlimited. The population will have favorable
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ºC) and an acidic pH. Enzyme C produced the most maltose at 37 ºC (body temperature) and a pH of 7 (neutral). Conclusion In a prior lab‚ it was given that the enzyme Aspergillus oryzae (fungus) works best at warm temperatures and an acidic pH‚ porcine (pig) works best at body temperature (37 ºC) and
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| | Kinetics Author: Katie Wood Instructor: Donald Kavanagh Chem 106b‚ Section 001 Lab Performed 8th‚ 2012 Lab Report Submitted February 22nd‚ 2012 Abstract The purpose of the lab was to determine the order of reaction for the dye Red #40. By measuring the reaction rate between bleach and the dye‚ the order of the reaction was determined to be first order. Introduction The study of kinetics is important for studying the amount of time it takes for a particular reaction to reach
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containing ψ from a beige precipitate that turned out to be mostly CA with some side products. The resulting supernatant was purified using reverse phase chromatography to isolate ψ as its DBU salt. Once dried‚ the crude product was ran through a cation exchange column using 0.1% formic acid to remove DBU to obtain ψ as its free acid form. Next‚ the purified compound was subjected to a Fischer esterification reaction with isopropanol to form the isopropyl ester under acidic conditions (Figure 5)
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The pGLO lab is a lab where students attempt to put the genes that make a jelly fish glow into E. Coli. After a process called transformation‚ the process in which a cell takes up and expresses a new piece of genetic information‚ the E. Coli will be able to glow and will be antibiotic resistant. The students first need to learn a couple of techniques before they are able to begin this lab. The first technique they will need is how to keep their environment sterile. They must learn to only open their
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This lab activity will go over environmental factors that affect the folded structure of proteins. We used a raw egg and noted the changes that happen when there were changes in temperature‚ pH levels‚ and salinity. Five eggs were cracked open and placed into different bowls with different solutions placed into each bowl. What we looked at was the process of denaturing of proteins seeing how it affected the composition of the raw egg between the yoke and white portion. My hypothesis for this experiment
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9/23/12 Lab Report #1 Meter Reading Summary The objective of this experiment was to learn how to read different meters like the D.C. volt meter and the D.C. amperes meter. In all meters each big line is a major division and each little line in between is a minor division‚ and if there is a line smaller than the minor division lines then that would be a sub minor division. Each meter has a low‚ medium‚ and high range. For example on the D.C. volt meter the ranges go from top to bottom 150‚ 15‚
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some 1-Hexene and petroleum ether present. To access the degree of the alkyl halide formed (1° or 2° halide) and to determine whether Markovnikov’s addition occurred‚ two qualitative tests can be used: (a) reaction with alcoholic silver nitrate solution and (b) reaction with sodium iodide in acetone. For the silver nitrate test‚ one drop of alkyl halide is added to 2 mL of 0.1 M solution of silver nitrate in
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Abstract: In this Lab we used the chemical DPIP to detect the rate of succinate broken down by the mitochondrial solution. We detected the amount of DPIP in the solution with a spectrophotometer and measuring the absorbance of light at the 600nm range. DPIP is a useful chemical to use in this experiment because it goes from a blue color when oxidized to a colorless liquid (Ogura‚ 281)‚ this is due to the hydrogen ions and electrons released during the transitional step between succinate and fumarate
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