Introduction The aims of the lab session was to familiarize with the principles‚ operation and application of a light spectrophotometer. This report discusses an experiment to study the relationship of absorbance and concentration‚ the interaction of electromagnetic radiant energy(ERE) and matter which is an important aspect of the Beer-Lambert ’s Law. This law states that the absorption of a compound is directly proportional to the concentration of that particular compound. This is because (a &
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choose. The study recruited HPS121 students to participate in a limited and extensive choice experiment in context to hotels. There were 661 participants‚ each chose one hotel and where then surveyed using six questions; three demographic and three in regards to difficulty‚ motivation and satisfaction using a 7-point Likert scale. The findings supported the hypothesis and were consistent with past research. Satisfaction and difficulty of choice showed a statistically significant difference with limited
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Abstract: In this Lab we used the chemical DPIP to detect the rate of succinate broken down by the mitochondrial solution. We detected the amount of DPIP in the solution with a spectrophotometer and measuring the absorbance of light at the 600nm range. DPIP is a useful chemical to use in this experiment because it goes from a blue color when oxidized to a colorless liquid (Ogura‚ 281)‚ this is due to the hydrogen ions and electrons released during the transitional step between succinate and fumarate
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Class Action Lab Report Purpose: To determine‚ through observations‚ the identity of substances produced in a chemical reaction. Materials: Safety Goggles Stirring rod Magnesium ribbon Solid CuSO4•H2O Matches Test tubes Copper metal 3M HCl Red and blue litmus strips 0.1M AgNO3 Zinc metal 0.1M Pb(NO3)2 Test tube holder 0.1M KI Bunsen burner 0.1M Na2NO3 Crucible tongs 1.0M NaOH Water (H2O) 0.1M Ca(NO3)2 50mL beaker 1.0 CuSO4 Procedure: Using the crucible
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Determination of the mole ratio of a chemical reaction The method of continuous variations is a means of determining the stoichiometric mole ratio of the reactants in a chemical reaction. The stoichiometric ratio‚ as given by the coefficients in the balanced chemical equation‚ represents the ratio at which chemicals must be combined to produce all product with no excess reactant. Since there is no “wasted” reactant‚ the maximum amount of product is made for the given amount of both reactants
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Abstract: This report presents the reason why the ocean does not freeze in the winter. Since the ocean consists of salt water‚ an experiment was done to test the effect of freezing on salt water compared to pure water. The experiment was conducted three times in order to obtain accurate results and eliminate errors. In addition to the salt and pure water experiment‚ a variation of this experiment was created to test other options. In the variation experiment the effect of freezing on sugar water
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carboxylic acids and alcohols with the presence of a catalyst. A carboxylic acid contains the -COOH group‚ and in an ester the hydrogen in this group is replaced by a hydrocarbon group of some kind. Sulfuric acid (H2SO4)is used as a catalyst for this reaction in order to accelerate the rate at which the product is formed. The general formula of an ester is RCOOR’ in which R is the alkyl group that comes from the carboxylic acid while R’ is the alkyl group that comes from the alcohol and COO is the functional
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Period : 4 Grade : 11 Enzyme Lab Report Question How heats effect the length of reaction time of an enzyme? Hypothesis I think the heat will make the length of reaction time of an enzyme become slowly. Heat is one of a way to denature the substrate. It means the heat will break down the structure of substrate in order the reaction of enzymes that we activated into it become slowly. Method for Collecting Data First I will record the length of reaction time with stopwatch when I pour
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Unknown Lab Report April 25th‚ 2006 Introduction The purpose of this lab was to identify two unknown bacteria cultures using various differential tests. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Whether the tests performed identified specific enzymatic reactions or metabolic pathways‚ each was used in a way to help recognize those specifics and identify the unknown cultures
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Introduction In the amylase lab‚ it was being tested if amylase‚ an enzyme found in saliva‚ would be denatured by being put in an acid or high temperatures. This lab is about denaturing amylase. It is tested by exposing it to pH and temperature changes. It is then mixed with Benedict’s solution‚ is a solution that changes color from blue to reddish brown when maltose is present. Amylase breaks starch into maltose‚ so is the amylase isn’t denatured‚ it should change colors. Amylase is an enzyme
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