Chem 51LC Experiment 6 Lab Report Aldol Reaction Purpose: The purpose of this experiment is to be able to conduct an aldol condensation reaction using an unknown aldehyde and an unknown ketone. H NMR is used to identify the unknown aldehyde and ketone. Melting point is used to identify the aldol condensation reaction. Theory: Condensation reaction is also known as a dehydration reaction. In the mechanism of condensation reaction‚ a bond is formed between two molecules and creates water as a
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The environmental factors that effected the rate of enzyme reactions were the enzyme concentration‚ pH‚ and temperature. These environmental factors help enzymes break down the poisonous chemicals into harmless substance. When we tested the liver with 2ml of hydrogen peroxide for a normal reaction it showed that it was exothermic. We added more hydrogen peroxide and the reaction rate of the liver was 3. We learned that the catalase is reusable because the liver reacted both times when we put in
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Enzymes Reactions to Changes in Substrate and Inhibitors Benjamin J. Mora Coronado University of Texas Rio Grande Valley at Edinburgh Abstract Purpose for the experiments was to test the enzymes in various scenarios and see how changing this would affect the rate of reaction. The enzyme source used in the experiments was Turnip Extract. Concentrations of Turnip extract for activity 1 where o.5ml‚ 1.0ml‚ and 2.0 ml as for the rest of the activities 2 Through 4 stayed at a consistent concentration
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BIO 211 Lab Section 11 February 15‚ 2012 Effects of Temperature on Enzymatic Activity Abstract Temperature is a measure of kinetic energy. As this movement increases‚ collision rate and intensity‚ and therefore reaction rates‚ increase. This experiment was conducted to determine if there is a minimum temperature that increase kinetic energy and denature enzymes to slow enzymatic reactions or fail to catalyze them. The experimental results indicate an increase in temperature will increase reaction
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extracellular solution‚ this was deemed the baseline. When we punctured each of the DEM‚ DEL1‚ and DEL2 crayfish muscles‚ we observed a large drop in voltage (refer to Figure 1)‚ therefore indicating that inside the muscle was more negative in relation to the outside solution. The time when the pipette was intramuscular‚ the recording showed a steady reading of the intramuscular voltage potential (Figure 1). When the pipette was removed from the crayfish muscle and was back in the extracellular solution‚ the
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proposed by Lowry et al. A bovine serum albumin stock solution (1mg/ml) was prepared in sodium hydroxide (1N). Five different concentrations (0.2‚ 0.4‚ 0.6‚ 0.8‚ 1 ml) of the prepared solution were taken in different test tubes. In another set of test tubes‚ 0.1 and 0.2 ml of the extract were taken. In each test tube‚ the volume was made up to 1 ml‚ followed by addition of the prepared alkaline solution (5 ml) at room temperature. The solutions were left undisturbed for 10 minutes. Then‚ 0.5 ml of
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Redox Problem Set 1: Reactions and Stoichiometry (All of these questions are no calculator friendly) 1) Give the oxidation number of carbon in each of the following: a) b) c) d) CF2Cl2 Na2C2O4 HCO3-1 C2H6 2) Give the oxidation number of sulphur in each of the following: a) b) c) d) SOCl2 H2S2 H2SO3 Na2S 3) Identify the oxidizing and reducing agents in each of the following: a) b) c) d) 8H+(aq) + 6Cl-1(aq) + Sn(s) + 4NO3-1(aq) SnCl6 -2(aq) + 4NO2(g) + 4H2O(l) 2MnO4-1(aq) + 10Cl-1(aq) + 16H+(aq)
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Introduction – Lab Report What is a Chemical Reaction? A chemical reaction is a change in matter that produces one or more new substances. A chemical change or reaction occurs when bonds are broken and new ones are formed. The formation and dissolution of these bonds are dependent upon environment changes. Even without the usage of microscopes‚ chemical reactions are usually apparent to the naked eye. The two main kinds of changes that one can observe are the formation of new substances and changes
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Lab report April 14‚ 2013 Abstract: In this article‚ we will experiment on the significant in strength of the enzyme by using three different test tubes and measuring the amount of product they give off. To determine this we are going to test the amount of color absorbance by using a special tool to help us understand our results. We will see how our end results show the effect of the amount of concentration we apply to each test tube. The results would be shown by the support of two graphs
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will have a negative charge and if pH < pI‚ the protein will have a positive charge. Buffer I has a pH >5‚ meaning both proteins carry a negative charge and bind to the DEAE (a positively charged resin). (b) pH = pKa + log10(Base/Acid) [Base = mM of sodium acetate; Acid = mM of acetic acid] = 4.7 + log10 (40/40) = 4.7 In order for the catalase to elute from the column‚ it must have lost its negative charge and stopped binding to the DEAE. Lowering the pH nullifies the negative charge on
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