Enzyme as protein Dr.Samina Haq Quantitative and qualitative test for protein and amino acids • 1. 2. 3. 4. 5. 6. Qualitative test Ninhydrin test Biuret test Xanthoproteic test Millons test Hopkins-cole test Nitroprusside test Quantitative test 1. 2. 3. Spectrophotometric assay Protein shows maximum absorbance at 280nm due to presence of tyrosine and tryptophane. Biuret test shows 540nm Lowry test shows 750nm Ninhydrin Test • Amino acid containing a free
Free Enzyme Amino acid Functional group
variety) for writing your answers. Leave 4 cm margin on the left‚ top and bottom of your answer sheet. Your answers should be precise. While solving problems‚ clearly indicate the question number along with the part being solved. Be precise. Write units at each step of your calculations as done in the text because marks will be deducted for such mistakes. Take care of significant digits in your work. Recheck your work before submitting it. 6) This assignment will remain valid for one year from
Premium Science Natural science Physics
Unknown Lab Report April 25th‚ 2006 Introduction The purpose of this lab was to identify two unknown bacteria cultures using various differential tests. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Whether the tests performed identified specific enzymatic reactions or metabolic pathways‚ each was used in a way to help recognize those specifics and identify the unknown cultures
Premium Enzyme Staphylococcus Staphylococcus aureus
Unknown Lab Report Unknown Organism #6 Ann Le (Phuoc) May 6‚ 2010 Dr. Carrington Microbiology Lab- MW 12:50 Le 1 I. Introduction My unknown organism #6 is Morganella morganii‚ which is a gram-negative bacillus rods commonly found in the environment and also in the intestinal tracts of humans‚ mammals‚ and reptiles as a normal flora. (3‚ 5) This bacterium Morganella morganii‚ was first discovered in the 1906 by a British bacteriologist named H. de R. Morgan. (2) Despite its wide
Premium Hydrogen sulfide Gram staining Agar plate
“2-7 Algebra Lab Percentiles” 1. Find the median‚ lower quartile‚ and upper quartile of the scores. Median: 16.5 Lower quartile: 10.5 Upper quartile: 23.5 2. Which performer was at the 50th percentile? the 25th percentile? the 75th percentile? 50th percentile: Arnold 25th percentile: Ingrid 75th percentile: Brooke 3. Compare and contrast the values for the median‚ lower quartile‚ upper quartile‚ and the scores for the 25th‚ 50th‚ and 75th percentiles. If you estimate the percentiles‚ then the
Free Median
Effects of Temperature on the Formation of Cheese Curds This lab was performed in order to find what temperature the enzyme chymosin is most effective at forming cheese curds. An enzyme is a protein that acts as a catalyst to speed up biological reactions in cells. They work by lowering the activation energy and help to orient the molecules correctly. Chymosin was originally taken from the stomachs of calves‚ and was called rennet‚ and then purified to make rennin. Eventually to catch up with
Premium Cheese Curd Cattle
Alhia Harris October 18‚ 2011 Biology Lab report Proteins are very important throughout the body. They have a lot of functions in cells. Antibodies are proteins that help cells rebuild the cells when they are destroyed. Transport proteins move substances from one place to another. Regulatory proteins control cellular metabolism in a way that cells throughout the body can have the right amount of glucose in the blood. A common protein is an enzyme; in cells they speed up chemical reactions. For
Premium Protein Amino acid
BIOS251 Week 7 Lab Exercise Joints To complete this worksheet‚ select: Module: Support and Movement Activity: Anatomy Overviews Title: Joints 1. a. From the main Joints page‚ click Fibrous Joints and identify each of the following structural joint types. A. Suture B. Syndesmoses C. Gomphosis b. Why are sutures and gomphoses classified as synarthroses? Both sutures and gomphoses are classified as synathroses because
Premium Joint Knee
Abstract: This report presents the reason why the ocean does not freeze in the winter. Since the ocean consists of salt water‚ an experiment was done to test the effect of freezing on salt water compared to pure water. The experiment was conducted three times in order to obtain accurate results and eliminate errors. In addition to the salt and pure water experiment‚ a variation of this experiment was created to test other options. In the variation experiment the effect of freezing on sugar water
Premium Water
Page 1/3 09/2010 SE 110.22 Forces in an Overdeterminate Truss The picture shows SE 110.22 in a frame similar to SE 112. * Comparison of forces in statically determinate and overdeterminate trusses1 * Bars with strain gauge full bridges to measure bar force1 * Computerised evaluation of experiments Technical Description Overdeterminate trusses are employed where overdimensioning is purposely required because safety must be maintained in the event of failure of an element‚ such as in aircraft
Premium Beam Force Truss