University of Texas at Tyler Lab 3C: Purification of L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally
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Reactions Enzymes are proteins found in living things that speed up chemical reactions. They aid in nearly all metabolic processes‚ such as food digestion‚ molecule synthesis‚ and the storage/ release of energy. An enzyme speeds up the rate of the chemical reactions by lowering the reaction’s activation energy‚ which means that by definition‚ an enzyme functions as biological catalyst. The activation energy is the energy that is used to get a reaction started. The function of an enzyme is dependent
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experiments are: Lactase Enzyme and Lactose. Lactase is a protein located in the human small intestine‚ while lactose is a term to define the sugar found in milk. Milk is a substance that contains nutrients necessary for our bodies‚ however‚ some people are unable to consume lactose related products‚ and so they are medically classified as Lactose Intolerants. The purpose of this experiment is to examine and illustrate the way that lactose gets affected by lactase enzyme whenever lactase is presented
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Lab Report An enzyme is a protein that speeds up the rates of chemical reactions. They recognize‚ bind‚ and change specific reactants. They do not change so they can catalyze the same reaction again and again. Activation energy is the amount of energy needed in order to begin a chemical reaction. A Catalyst is a substance that increases the rate of a chemical reaction without itself undergoing any permanent chemical change. Catalysts are substances or a substance that configures another substance
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INTRODUCTION There are many reasons for knowing the identity of microorganisms. The reasons range from the knowing the causative agent of a disease to knowing the correct microorganism in order to make antibiotics. This study was done by applying the following methods; OF Glucose‚ Indole Production‚ and Malonate Utilization test for the identification of an unknown bacterium. The methods will assist in determining the unknown bacterium found in a 55 year old male that was passing blood and mucous
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Enzymes (pron.: /ˈɛnzaɪmz/) are large biological molecules responsible for the thousands of chemical interconversions that sustain life.[1][2] They are highly selective catalysts‚ greatly accelerating both the rate and specificity of metabolic reactions‚ from the digestion of food to the synthesis of DNA. Most enzymes are proteins‚ although some catalytic RNA molecules have been identified. Enzymes adopt a specific three-dimensional structure‚ and may employ organic (e.g. biotin) and inorganic (e
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Introduction: During this unknown lab report various test were performed to differentiate microbes from each other and to compare metabolic and biochemical process. The gram stain distinguishes between Gram positive and Gram negative bacteria based on the composition of the cell wall. The Gram stain procedure distinguishes between Gram positive and Gram negative groups by coloring these cells red or violet. Gram positive bacteria stain violet due to the presence of a thick layer of peptidoglycan
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PEES 4200W- Physiology of Exercise Lab #2: Metabolism and Energy Expenditure 1. The Respiratory exchange ratio is the ratio of the volume of carbon dioxide produced and oxygen consumed. The ratio indicates the energy that the subject is expending for indirect calorimetry‚ how efficient the subject ’s body is at utilizing the oxygen inhaled‚ as well as the main substrate being used for energy during varying intensities of exercise. During rest the volume of carbon dioxide was 0.73L/min‚ the volume
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Determining the Concentration of a Solution: Beer’s Law Purpose: The purpose of the experiment is to determine the concentration and formula of an unknown cobalt nitrate solution by measuring absorbance. Introduction: A Colorimeter will be used to determine the concentration and formula of an unknown cobalt nitrate solution. The colorimeter sends blue light from the LED light source to pass through the solution and hit a photocell. A solution with a higher concentration will absorb more
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Determining the Concentration of an Unknown Solution Graph: The effect of concentration of CuS04 * 5H20 on transmittance. I solved for my unknown by plugging in “y” as my given transmittance value‚ which was 85.0. y = -278x + 100.12 .85 = -278x + 100.12 -99.27 = -278x X = .357M (concentration) The relationship between the transmittance values and the concentration is an indirect relationship. Whenever the concentration increases‚ the transmittance decreases. It can also be reversed
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