Enzyme kinetics (catalase/kmno4) Enzyme catalysis Farah Mohamed galal 22-3014 t09 Introduction: E + S → ES → E + P Enzymes are proteins which act as a catalyst in biochemical reactions(affect the rate of achemical reaction). The substrate binds to the active site of the enzyme. Any deformation of the active site will affect the activity of the enzyme‚ these are some ways that enzyme action may be affected because of them: 1- Salt concentration: If it is close to zero or very high
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Lab Report: Stoichiometry Lab Oct. 27‚ 2011 Claire Elizabeth Lab Partners: Hannah Signature:___________________ Introduction- Baking soda and vinegar are two common materials found in almost every household. That‚ plus the fact that all the starting and finishing materials are non hazardous and safe‚ is why this is one of the first chemical reactions that many people are exposed to The purpose of this experiment tests which of the two reactants (vinegar and baking soda) is the limited
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Microbiology-2460 Lab-003 March 31‚2008 Lab Report-Escherichia coli Abstract The purpose for this lab report was to identify and inform of an unknown bacteria that has been causing a patient to have lower abdominal and pelvic pain. To obtain the identification of this unknown bacterium‚ several biochemical tests needed to be performed in order to prescribe the correct medication to treat and cure the symptoms. Introduction In a lab today‚ I am to identify an unknown bacterium that is
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LSM1102 Lab Report Introduction Transformation is a process which involves plasmid DNA being bound to the cell surface and the subsequent uptake of DNA by the cell (Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin)
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structure of the enzyme is mainly dependent on the active site and variable groups. Extreme temperatures or extreme pHs can alter the structure of an enzyme. Enzymes function to lower the activation energy to break the bonds. They achieve this by putting stress and pressure on the bonds or creating a microenvironment for the substrate. Enzymes are regulated by inhibitors or activators and can be inhibited by the products of the reaction‚ called feedback inhibition. Enzymes are catalytic proteins;
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Lab Report Abstract: The purpose of this lab was to see how magnesium reacts with oxygen. This reaction must be forced with heat. As magnesium changes to magnesium oxide the mass increased. Procedure: 1. Set up the stand and bunsen burne. 2. Go to crucible heat oven to obtain a crucible. 3. Weigh the crucible and lids mass. 4. Break up the magnesium ribbon on the crucible and weigh the mass of the magnesium‚ crucible‚ and lid. 5. Place the crucible on the ring stand over the flame. Allow
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Enzyme Catalase Activity in Reaction with the Substrate Hydrogen Peroxide Abstract We performed these experiments to observe the effects of enzymes on the rate of reactions. We tested and compared the activity of the enzyme catalase on the substrate H2O2 in various states and percentages‚ and observed the absorption values of the enzyme-substrate relationship at different concentrations. Our results show that the more substrate available‚ the quicker the reaction will happen except in one test
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Lab Report Janice Chisholm SCI207: Dependence of Man on the Environment Instructor: Lee Ott April 28‚ 2013 1. What patterns do you observe on the information table 4? While observing the information in table 4‚ it appears that the number of fish changes from time to time and the oxygen increases and / or decreases when this occurs. 2. Develop a hypothesis relating to the amount of dissolved oxygen measured in the water sample and the number of fish observed in the body of water?
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Year 11 Lab Report Template Experiment – Modelling A. Title • A brief concise descriptive title B. Aim • What are you trying to find out? • What do you want to learn? C. Hypothesis • Write a possible solution to the problem. • If ………………………………………………………………………………………….then ………………………………………………………………………………………………………. • Make sure the above statement is testable. • The Independent variable is ……………………………………………………..
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Tim Readmond AP Biology Lab Report I. Title a. Modeling Diffusion and Osmosis II. Introduction a. If one places a 1.0 M solution of glucose inside a bag and then places that bag into a beaker containing a 1.0 M solution of sucrose‚ the percent of mass lost in the bag is 10.5%. The solution in the bag is hypertonic while the solution in the beaker is hypertonic‚ which is why water moves from the bag to the beaker and the bag loses mass. b. The purpose of this experiment is to see whether
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