Temperature and Enzyme Activity Aim: To investigate the effect of temperature on enzyme activity Hypothesis: As the temperature deviates from 40°C the activity will lower Equipment: – Chemicals: – Milk – Junket tablets – Hot water – Ice – Test tubes – Stopwatch – Measuring cylinder Risk Assessment: |Hazard |Risk |Prevention | |Hot Water
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12 Biology (SBI 4U1) Nicole Mikulis Unit: Biochemistry Sept 14 2012 Lab: Effect of temperature and pH on catalase activity BACKGROUND Catalase is an enzyme that detoxifies chemicals that might harm the cell such as hydrogen peroxide (H2O2). The enzyme breaks H2O2 into water and oxygen. The production of the oxygen gas bubbles serves as evidence that the catalase enzyme is working. As catalase is breaking the bonds between H2O2‚ it is releasing energy in the form
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The data from the experiment demonstrates that the catalase enzyme breaks down the hydrogen peroxide due to its harmful toxicity to the liver. In section A‚ the effect surface area has on the enzyme was tested. The results have proven that as the surface area increases‚ the reaction rate of the enzyme also increases. To illustrate‚ when the liver was ground‚ the bubbles from the reaction reached a maximum height of 150mm in five seconds less than the unground liver which merely reached a maximum
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DISCUSSION: Bromelian added to Gelatin: Bromelian is an enzyme found in pineapples. When Bromelian is added to gelatin it breaks down the protein and does not allow the gelatin to solidify. There are several factors that can cause an enzyme to slow down or to completely stop reacting. For example‚ temperature and pH can effect enzyme activity. Canned pineapple juice and fresh pineapple juice were used to see how the enzyme would react differently. In fresh pineapple juice the Bromelian have would
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Potato Cell Cytoplasm Composition PURPOSE: To determine the concentration of salt in the cytoplasm of potato cells HYPOTHESIS: Increase in salt concentration results in a hypotonic solution where the mass of the potato increases due to the movement of water into the cell. On the other hand‚ decrease in salt concentration results in a hypertonic solution where the mass of the potato decreases due to the movement of water outside the cell. MATERIALS: Potato Forceps Stopwatch Scalpel Test
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Title: Potato Osmosis Abstract: In this experiment was designed to study the effect of a concentrated solution applied to white and sweet potatoes. The solution’s impact among the potatoes were to be either hypertonic‚ hypotonic‚ or isotonic. The potatoes were first cut into fries with a fry cutter‚ and then weighed after being cut. The white and sweet potatoes’ weights ranged from .005-.015 grams. A total of 18 sugar solutions are to be made over the course of 3 trials. In each trial‚ 6 different
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pH and Chromatography Lab Report Kevin Rivera Biology Mr. Langley 2C 10/7/13 Introduction In this lab‚ of pH and Chromatography‚ in the pH aspect of the lab we are trying to figure out the pH level of certain chemicals by writing down of known solutions to find the type of unkown solution using pH standards. In the Chromatography‚ we are trying to separate the chemicals using water and a piece of paper. Methodology Materials: Safety Goggles pH indicator & pH indicator key
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Affect of enzyme concentration to the rate of reaction Aim: With the experiment of protein solution‚ in this case egg white added to different pepsin concentrations (0%‚ 0.2%‚ 0.4%‚ 0.6%‚ 0.8%‚ 1.0%) shows‚ as the egg white is a protein and the pepsin works as an enzyme‚ how a higher pepsin concentration and therefore a larger amount of enzymes effect the rate of reaction. Hypothesis: An increased concentration of pepsin speeds up the time the mixture needs to come clear. Introduction:
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Effect of temperature of the reaction: The effect of the temperature of the reaction on the activity of the purified enzyme was carried out by make the enzymatic reaction for 10 minutes at different temperature 25‚30‚35‚40‚45‚50‚60 and 70°C using an enzyme protein 0.1mg/reaction mixture and substrate concentration of 15 mg/reaction mixture‚ using a control of previously heated enzyme solution in the reaction. The data recorded in (table 27) and (figure 29) illustrate the effect of temperature of the
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cell‚ so the water molecules diffused out of the cell through the selectively permeable membrane. The higher the concentration‚ the more plasmolysed the cell becomes and my results show this. The 0M bathing solutions in both experiments caused the potato cells to become slightly turgid‚ this means that there was a higher concentration of solution on the inside of the cell. So the water molecules diffused into the cell through the selectively permeable membrane. The rate of water loss decreases as
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