Lab Topic: Movement of Materials Across the Cell Membrane—Potato Tuber Cells Introduction: Osmosis is a form of diffusion in which water diffuses through a selectively permeable membrane from a region of high concentration to a region of lower concentration. This form of diffusion takes place when the molecules in high concentration are too large to move through the membrane. Three factors that determine cell membrane permeability are shape‚ size‚ and polarity. For this experiment‚ it must
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inside of the amylose coil.The amount of blue complex that starch gives with iodine can be measured by using a spectrophotometer. α-amylases are found in saliva‚ pancreatic juice‚ human breast milk‚ serum and certain tissues such as the liver. This enzyme catalyzes the hydrolysis of α (1-4) linkages in starch by breaking it down to maltose and some glucose. As the starch is broken down‚ the coiled structure of α-amylase is unfolded. Therefore‚ iodine will no longer be able to form the blue complex
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Reminder: All post labs need to be 1- typed (not handwritten) ‚ 2- original (not copied from a classmate)‚ 3- answered using complete statements and 4- turned in at the beginning of the lab. Post-lab questions for Topic 5 – Enzymes Name: Date: Group: T W R Formation and Detection of Benzoquinone Table 1. Formation and Detection of Benzoquinone: Record Absorbance Time 2A-Potato extract + cathecol 2B- Potato extract + water 2C- Catechol + water After 10 min 1- What were the substrate
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How to Write a Lab Report Lab reports are formal papers that describe and analyze lab experiments. They follow a very specific format. If you study this paper carefully and follow its directions‚ you will find that these papers are not at all difficult and can have a positive impact on your Biology grade. Paper formatting: The paper should be typed and double spaced. Use a standard 12 point font (do not use cute fonts or colored paper!). Except for the title and abstract – each section
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1.7 Factors that affect the activity of an enzyme It is important when working with enzymes to understand basic enzymatic theory behind them when selecting conditions to measure the activity of the enzymes. The factors that are known to affect the concentration of enzymes are temperature‚ pH‚ concentration of enzyme‚ concentration of substrate‚ buffer type and concentration‚ the presence of any inhibitors and cofactors (Worthington-biochem.com). 1.7.1. Temperature With most catalysed reactions‚
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Finding the Optimal pH of the Enzyme Peroxidase with the Aid of a Spectrophotometer ABSTRACT The peroxidase enzyme was partially purified‚ and the enzyme activity was calculated with the use of a spectrophotometer‚ demonstrating the effect of pH on peroxidase activity. The objective of this exercise was to determine the ideal pH for peroxidase activity. The data concluded that the optimum pH for peroxidase activity is 5‚ and the “standardized” enzyme volume is 2.8mL.
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Ayaan Nadeem SBI 4UO-A Tuesday‚ February 27‚ 2013 Ms. Balmer Pineapple Jelly Enzyme Lab Discussion After completing the Pineapple Jell-O Enzyme lab‚ the final results were that the canned pineapple formed the jelly while the fresh pineapple did not. Pineapple In order for this to have occurred‚ there has to be a comparison between fresh and canned pineapple in terms of their physical and chemical properties. The physical properties of fresh pineapple are that it is sweet‚ ripe and raw.
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Introduction: The purpose of this experiment is to measure the effects of changes in temperatures and pH on enzyme activity in skeletal muscle‚ particularly the activity of lactate dehydrogenase (LDH). LDH is a glycolytic enzyme which converts pyruvate to lactate in the following equation: LDH Pyruvate+ NADH ------------ Lactate + NAD The reaction above can move in both directions‚
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THE EFFECT OF pH LEVEL ON CORROSION RATE A Long Laboratory Report Presented To; The Department of Chemical Engineering BY
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Effects of hyperventilation on CO2 and pH levels Alidrin Armandico Physiology Lab 142 Egle Ortega Aprill 25‚ 2014 Abstract The aim of this experiment was to analyze how pH and carbon dioxide (CO2) levels in the blood change during hyperventilation and how the renal system compensates for this condition. Hyperventilation decreases CO2‚ increases pH‚ and lowers H+ levels which then results to respiratory alkalosis. In the experiment‚ we used the PhysioEx 8.0 software to simulate hyperventilation
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