Escherichia coli Microbiology Lab NAS 185-14L Introduction In this lab experiment we did several test to determine what our unknown bacteria was. To determine this we recorded the results of how the bacteria reacted to different media. Depending on the results of each test we could narrow down the different bacteria to determine what our unknown is. This experiment will also determine if our bacteria is a fermenter of sugars and if it is catalase positive. If the bacteria is
Premium Escherichia coli Bacteria Agar plate
Macromolecules Lab Purpose: to determine a method of testing for macromolecules. Materials: Knowns: Unknown: Test Solutions: Glucose solution Unknown solution Iodine solution Gelatin solution Benedict’s solution Starch solution Biuret solution Oil Brown paper Water Procedure: 1. Create a data table. 2. Label 5 test tubes with known solutions. 3. Add 10-20 drops of each known solution to respective test tubes‚ do not mix pipets! 4. Add 3-5 drops of
Premium Chemistry Bacteria Experiment
Phelps‚ Biology 205 MW 2:10pm. Unknown Organism Paper The unknown organism tested in the Mesa lab was collected from in between the toes of my puppy‚ Riley. The original culture grown consisted of dozens of visibly different organisms of varying colony colors and growth patterns. I chose to test a smaller‚ red colony for my experiments. Based on its gram reaction and oxygen requirements‚ the following tests were performed to reach my presumptive ID: Test Performed Result Gram Stain +
Premium Bacteria Microbiology Staining
Bacillus‚ Mycoplasma‚ and Escherichia In a clinical setting signs and symptoms can lead a care taker down a treatment path‚ but if a bacterial infection is suspected‚ a definitive answer from the “lab” is needed before prescribing medications. In this instance a yellow sputum was produced by the patient‚ which will be taken to the lab an analyzed under a microscope. There are three suspect bacteria‚ which are Bacillus‚ Mycoplasma‚ and Escherichia. A discussion of staining procedures‚ as well as
Premium Bacteria
-Unknown Mixtures Lab- Purpose: The aim of this experiment was to find out the two substances of the unknown mixture from salt‚ sugar‚ dirt‚ and baking soda by looking at its properties. Hypothesis: Find the unknown substance by testing it out by heating it‚ pouring water‚ vinegar‚ and observing it . Procedure: 1) Materials- * 100ml of water * Vinegar * Bunsen Burner * 2 beakers * 1 spatula 2) First‚ do steps (3-6)to find out the properties of salt‚ sugar
Premium Chemistry Chemical substance Water
Conclusion: (Identity‚ % error‚ other evidence‚ how to improve) The identity of Unknown A is the compound cobalt(II) chloride hexahydrate (CoCl₂•6H₂O).This is justified by the similarities in color of red-purple crystal like grains‚ which is significant in identifying the product out of the eight suggested identities. Only two had similar characteristics which were CoCl₂•6H₂O and CoSO₄•6H₂O. Before unknown A was heated‚ there was a notable common physical property of the substance being red crystal
Premium Chemistry Chemical substance Ion
Sp2012 (Set all of your margins to ½”) Purpose: The purpose of this lab is to help you become a little familiar with some of the tests that can be typically performed in a clinical or research lab facility. These tests may help in determining a particular pathogen’s growth needs. There are several sections to this lab. Find each section and complete the “Preparing for Class” sections. Preparing for class - Day 1 Read in your lab manual about the following agar mediums: Blood Agar (pg 168)‚ EMB
Premium Staphylococcus Growth medium Escherichia coli
| Gram Negative Unknown | Biology 3444-006 | | Lena Wallace | 11/7/2011 | | Abstract: The purpose of this lab was to identify an unknown bacteria culture using differential tests. The identification of the unknown culture was accomplished by identifying the bacteria based on its specific metabolic characteristics and morphology. It is suggested that culture 11 is a sample of Enterobacter aerogenes. Introduction: This experiment was centered on metabolic and biochemical testing
Premium Staining Gram staining Enzyme
Purpose: Unknown mixtures will be separated by means of chromatography in which the mixture will be passed in a solution through a medium leaving behind components of the mixture at different rates‚ therefore‚ different spots on the absorbing substance. This will help determine the identity of unknown mixtures. The spot colors on the strip of filter paper and the Rf values of the unknown samples will be compared to those of known samples. To find the position of the spots on the strip of paper‚ we
Premium Chemistry Chromatography Chemical substance
The first test conducted on unknown bacteria 32 was the Gram stain. From this stain‚ unknown 32 was found to be a Gram-positive cocci. This test eliminated all possible Gram-negative bacteria‚ Gram-positive rods and Gram-positive spirillium. Next‚ the endospore test determined whether or not the Gram-positive bacteria contained endospores. With the use of malachite green‚ steam‚ and safranin it was found that unknown bacteria 32 did not contain endospores. This eliminated Gram-positive cocci Sporosarcina
Premium Bacteria Microbiology Staining