CHM 456 PREPARED BY : AHMAD FAIZ ZIKRI BIN ALIAS GROUP AS 202 2M1 DATE 16/3/2015 TIME 3.10 PM – 6.00 PM LECTURER’S NAME Dr. rer. nat MOHD TAJUDIN MOHD ALI OBJECTIVE: To separate a mixture of benzoic acid and a neutral compound (triphenylmethanol or 1‚2‚4‚5-tetrachlorobenzene) into its components by extraction and also to get the melting point. THEORY: Extraction is a process of transferring a solute from one solvent to another. In this experiment‚ a mixture of benzoic acid and a neutral
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FHSB 1214 BIOLOGY 1 FOUNDATION IN SCIENCE Name NG KEI PENG Group member Practical Group P20 Date of lab class Program Foundation in Science Unit code FHSB1214 Unit description Biology I Year and trimester of study 2015‚ Trimester 1 Title of lab report Investigation of the Effects of Different Catalytic Conditions on Hydrogen Peroxide Decomposition. Lecturer’s name Norkhalidah Binti Jamali Investigation of the Enzymatic Effects of Different Catalytic Conditions on Hydrogen Peroxide Decomposition
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First thing is to make your hypothesis on how to filter dirty water like mine “My hypothesis is if I use one cut bottle and layer it to be pebbles‚ sand‚ cloth‚ charcoal‚ then cloth‚ sand‚ and pebbles it filter the water will be cleaned.”. Then you start to build hypothesis‚ so start by getting a bottle‚ cut the bottom of the bottle like two inches of it. You take the cap off the bottle then get two coffee filter and use two rubber bands to tie it to the cap of the bottom. Then add about a inch of
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Procedure. 1) Accurately weigh approximately 2 g of the mix and dissolve it in 50 mL of diethyl ether. Be sure to dissolve the entire mix since it is not homogeneous. 2) Pour the solution into a separatory funnel. 3) To extract the nicotinamide from the mixture‚ add 5 mL of 5% HCl and shake gently. Draw off the lower aqueous layer into 125 mL Erlenmeyer flask and repeat the extraction with a second portion of HCl. Combine the three extracts and then set this mixture aside. 4) To separate the
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1. Obtain 1 gram of an impure‚ unknown substance solid. Make sure to stir the mixture before measuring the sample; record the mixture’s code in the data section. 2. Add approx. 25-50 mL of water and several boiling chips to a 125 mL Erlenmeyer flask and heat the water to a gentle boil using a hotplate. 3. On a second hotplate‚ place a 125 mL Erlenmeyer flask containing the one gram of unknown solid along with a boiling chip. 4. Using a ring clamp‚ slowly pour approx. 5-10 mL of boiling water into
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3-2-3-1-Conventional Culture Method (Marks 1972): The infected lesions of the tuberculin positive reactors were collected and placed in to a sterile mortar containing sterile sand. The fat was removed and the suspected parts were cut into minute pieces. Then 2 ml of distilled water added to the mixture‚ homogenized and crushed well until the suspension was obtained. Then 2 ml of H2So4 acid 4% added to the mixture then incubated the mixture at 37 0C for 1/2 h. The mixture was diluted with
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For this experiment‚ we started off by taking tubes numbered 1-4 and started adding one scoop of our enzyme catalyst‚ in this case‚ the yeast. We then proceeded to measure and add 1 mL of distilled water to test tubes A-D. To get a more accurate measure of 1 mL of distilled water‚ we used the dropper labeled “W” to drop distilled water into the 5 mL graduated cylinder until we saw that the bottom of the water line reached closely to 1 mL. Next‚ we took the four tubes with the scoop of yeast and
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2.3. Method Development 2.3.1. Preparation of Stock Solution 100 mg of the synthesized compound was made up to 100 ml into a 100ml standard flask with ethanol to give a solution of 1000 μg/ml. 2.3.2. Determination of λ max 1 ml of stock solution was pipetted out was made up to 10 ml into a 10ml standard flask with ethanol to obtain strength 100 μg/ml and scanned at 200-400nm using a UV spectrophotome-ter (Deepak V Bageshwar et al.‚ 2010). 2.3.3. Preparation of Standard Calibration Curve Aliquots
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1. Ventilate the work area well‚ cover the work space with newspaper‚ and put on gloves and goggles. Be sure pets and kids are not running underfoot as begin this project. 2. Start by measuring the oils and placing them into the crock pot. Remember: For this recipe measuring all ingredients by weight‚ not by volume. 3. Turn the crock pot on high and melt all the oils. Better to use a smaller crock pot to cook the ingredients down and then move to a larger one once start adding the liquids. 4. Place
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To begin the experiment‚ the reaction apparatus was assembled (as shown in Figure 1 below from the lab manual) consisting of a 3.0 mL conical vial charged with p-cresol (80uL from Eppendorf pipette)‚ 25% aqueous NaOH (130uL)‚ and a spin vane. The solution was mixed thoroughly and tetrabutylammonium bromide (9mg) was added along with n-propyl iodide (75uL) and it was equipped with a flask with a water reflux condenser. The solution was heated (95-100°C) while vigorously stirring it. After 60 minutes
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