determine the unknown DNA plasmid using restriction enzymes and conducting electrophoresis finally comparing the resulting fragments with the known restriction map. In this lab‚ it succeeds in showing the fragments. In this report we will discuss the‚ results‚ limitations and possible errors. Introduction In biology restriction enzymes are used in several ways to modify and manipulate DNA molecules. One common use is to compare pieces of DNA from one that is unknown‚ with fragments of DNA from another source
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Project #3: Explanatory Synthesis Paper Comic from: http://www.lapsura.com/drawings/archives/images/do-my-essay.gif REVIEW Chapter 4 WRAC (See Notes on Explanatory Summary below) For the academic explanatory synthesis paper you will read a minimum of three sources on the topic you choose. You can choose from three topics: ➢ Fairy Tales: A Closer Look at Cinderella: (Select three articles in textbook) See Syllabus/Calendar ➢ E-Mail Etiquette: (Select
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the structure of DNA they wanted to use the information to help them identify how DNA is replicated. Three different theories of replication were proposed by Watson and Crick. The semi-conservative model‚ where the DNA strand splits into two halves‚ which will then create a new DNA strand consisting of the old original half and a new half. The conservative model where the whole of the original DNA strand acts as a template and is replicated to make a completely new strand of DNA. The dispersive model
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DNA is the most important for life One of the most complex and mysterious aspects of contemporary science is the constitution of life. While it goes without saying there are an enormous amount of components that collaborate to achieve the most effective and efficient components of life‚ it’s also clear that some of these molecular components contribute to the actual function of living system more than others. This essay argues the DNA is the most important molecule for life. Deoxyribonucleic
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for the DNA ligation‚ in which the gene products with sticky ends will be inserted to a plasmid vector. Also‚ to transform E.coli DH5α cells by introducing the plasmids DNA which contains the gene of interests into the E.coli strain(DH5α). The plasmid DNA can replicate inside the transformed E.coli DH5α cells‚ only successful transformed cells can produce the protein that is resistance to kanamycin‚ this allows for the selection of successful transformed cells. 2. Overview of experiments DNA purification
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Experiment 13: Synthesis of 1-Bromobutane An SN2 Reaction Experiment #13: Synthesis of 1-Bromobutane an SN2 Reaction Introduction: In order to synthesize 1-Bromobutane an alkyl halide must be present to undergo a nucleophilic substitution reaction of an alcohol. Since 1-butanol is a primary substrate it will undergo an SN2 reaction with sodium bromide in order to convert the alcohol group to water which is a better leaving group and will in the end produce 1-bromobutane. Experimentally
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So now that we know what recombinant DNA is‚ we are going to look at its purposes. ******** Recombinant DNA is used to insert a gene for production of a protein of interest The production of heat and drought resistant crops can alleviate world hunger around the world. Production of clotting factors can treat bleeding disorders such a Haemophilia which casues bleeding into the joints and can be life threatening. Hepatitis B vaccines are made with the help of yeast cells It is used for the production
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7.1.1 Describe the structure of DNA‚ including the antiparallel strands‚ 3’-5’ linkages and hydrogen bonding between purines and pyrimidines. DNA is made up of two strands. At one end of each strand there is a phosphate group attached to the carbon atom number 5 of the deoxyribose (this indicates the 5’ terminal) and at the other end of each strand is a hydroxyl group attached to the carbon atom number 3 of the deoxyribose (this indicates the 3’ terminal). The strands run in opposite directions
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this lab was to isolate DNA from a food sample‚ amplify the DNA using a polymerase chain reaction‚ and test the amplified DNA for the presence of the Bt gene or the 35s promoter. In part one of DNA isolation‚ the food sample was crushed before Lysis Buffer was added‚ in part to break down some cell walls‚ but also to increase area of the food sample being touched by the Lysis Buffer. The purpose of Lysis Buffer is to break down the cells in the food sample and release their DNA into the solution. By
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Synthesis Essay Gender Identity‚ Stereotypes‚ & Inequalities: Problems & Solutions Directions: The following prompt is based on the accompanying sources. This question requires you to synthesize a variety of sources into a coherent‚ wellwritten essay. When you synthesize sources‚ you refer to them to develop your position and cite them accurately (you should cite both direct and indirect references). Your argument should be central; the sources should support the argument. Avoid summarizing sources
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