Oxygen Requirements of Bacteria BACKGROUND The GasPak system is useful for culturing anaerobic bacteria on standard microbiological media because the GasPak generates carbon dioxide and hydrogen. The hydrogen will combine with oxygen present in an anaerobic jar to produce water. This system can reproducibly attain oxygen levels in the parts per million range if used correctly. This is the best method for determining the oxygen requirements of unknown organisms. A candle jar is useful
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Title: Staining Introduction: Microorganisms are small and colorless‚ invisible to unaided eyes. When observing them under microscopes‚ we use various methods to make microbes apparent. One of the most important methods is staining. Staining techniques play an essential role in the studying of microorganisms; they help to reveal characteristics of microbes‚ such as their morphologies‚ sizes‚ arrangements‚ chemical components and many more. The purposes of the experiments are to be
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sterile swab obtained sample of bacteria from skin then transferred using the aseptic transfer technique into the sterile media. Incubated both specimens for 48 hours observed and recording results of growth at 24 and 48 hours. After observing final growth pattern at 48 hours prepared both wet mount and direct stain slide for each of the cultures. Viewed under microscope using both the 40X and 100X oil immersion lens. Disinfected work area. Observations: |Bacteria
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repositories of bacteria/microbes ⁃ borne sterile ⁃ microbe on all surface area of the body ⁃ sterile areas: eyes‚ brain‚ spinal cord‚ bones‚ kidney‚ internal organs ⁃ mutualistic relationship: we provide site and nutrient and microbes provide vitamin‚ aid in food digestion ⁃ division of microbial world ⁃ living component: organism ⁃ prokaryotic ⁃ nucleoid area ⁃ one circular chromosome ⁃ non-membranous organelle ⁃ cell wall: peptidoglycan ⁃ binary fission ⁃ bacteria simple‚ dynamic
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There are various reasons why we observe children in schools. We make objective decisions and assessments about pupils; • Behaviour • Learning styles • Levels of development • Skills • Learning achievements • Learning needs/future goals We need to see how they interact with other children and adults and how they perform against each other. There are two main types of observation – informal and formal. With the informal observation you may observe a child in the classroom‚ out on the playground
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Lab: Sampling Bacteria Purpose: Refer to handout sheet. Materials: Refer to handout sheet. Procedure: Refer to handout sheet. Pre-Lab Questions: 1. Why is one dish being reserved for the class as a "control"? Having a controlled variable is important in order to be able to look at what the bacteria would look like if it hadn’t been contaminated and just left as agar. Having a sample of agar that wasnt exposed to any bacteria will provide a clear picutre of what grew on the agar
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Identification of Microorganisms • For many students and professionals the most pressing topic in microbiology is how to identify unknown specimens. • Why is this important? • Labs can grow‚ isolate and identify most routinely encountered bacteria within 48 hrs of sampling. • The methods microbiologist use fall into three categories: ♣Phenotypic- morphology (micro and macroscopic) ♣Immunological- serological analysis ♣Genotypic- genetic techniques Dr.T.V.Rao MD 2 Microbe Identification
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micro-organisms that are in the human digestive system. Article of choice‚ (Adnan‚ 2010) Benefits of Microorganisms to Humans‚ Retrieved February 8‚ 2013. This article was of interest because there are many bacteria that we encounter in our daily lives and it was informative to know that all bacteria is not bad. In fact‚ there needs to be a balance between the two and by educating yourself with the smallest amount of new information is very helpful. Humans rely on micro-organisms for a variety
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Sarah Burns Evolution of Plague Bacteria The Bubonic Plague otherwise known as the Black Death‚ has gotten most of its attention from medieval paintings‚ poetry‚ and journals of revulsion. The real horror‚ was the disturbing biological evidence of the bacteria that caused all the pandemics‚ known as Yersinia pestis. The pathogen got its name from the two investigators Yersin and Kitasato. In 1894‚ Yersin was known as the main investigator (ergo. Named after him)‚ he claimed that the mice/rats were
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difference between bacteria‚ viruses‚ fungi and parasites are: Bacteria are single celled organisms that can rapidly multiple themselves every 10 minutes up to 10 times each bacterial cell‚ but they do not live or reproduce in a human cell. When threatened they will make a copy of their DNA to enable them to come back to life in the right conditions. They are able to survive in most extreme living conditions including with and without oxygen‚ there are various types of bacteria such as Cocci/Coccus
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