Distillation Introduction: The recrystallization technique you learned last lab is a method for purifying solids. This week we will learn how to perform a distillation‚ a method for purifying liquids. Distillation is a common wet-chemical technique for separating organic compounds based on differences in boiling points. Upon heating a mixture of organic compounds‚ the more volatile compounds (those with the lowest boiling point) will vaporize first (i.e. be converted to gases)‚ leaving the higher-boiling
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Electrophoresis of Proteins from Cauliflower Fractions by SDS-PAGE Laboratory Summary Electrophoresis is a technique where molecules are separated according to their physical properties such as size‚ charge‚ and/or shape. Charged proteins are commonly separated in this matter using PAGE (polyacrylamide gel electrophoresis) to identify individual proteins present in samples. In this lab‚ SDS-PAGE was used. SDS-PAGE separates charged proteins primarily by size because the ionic detergent sodium dodecyl
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percentage of hexane and toluene at fractions near the beginning and end of our distillations. Relative percentages have been recorded in the table below‚ and our calculations are shown on page 5. Simple Distillation Retention time (s) Fractional Distillation Retention time (s) Cyclohexane Retention time (s) Toluene Retention time (s) Cyclohexane Retention time (s) Toluene Retention time (s) Fraction 1 (4th mL) 15 25 14 24 Fraction 2 (17th mL) 14 26 16 25
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(Luna Production‚ n.d). In this lesson‚ kindergarten students group collections of objects into groups of five or ten and record their collections on paper. The second snapshot lesson is What Fraction is This Shape Red? (Ryan et al.‚ n.d). The students in this lesson learn the abstract concept of fractions
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THE REAL NUMBER SYSTEM The real number system evolved over time by expanding the notion of what we mean by the word “number.” At first‚ “number” meant something you could count‚ like how many sheep a farmer owns. These are called the natural numbers‚ or sometimes the counting numbers. Natural Numbers or “Counting Numbers” 1‚ 2‚ 3‚ 4‚ 5‚ . . . * The use of three dots at the end of the list is a common mathematical notation to indicate that the list keeps going forever. At some point‚ the
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First‚ the buffer was prepared by using the formula as follows: Figure 1: Calculation for prepare 0.1 M potassium phosphate buffer at pH 6 3.4007g of potassium phosphate was weighed and placed in 300 mL beaker. Then‚ 125 mL of water was added into the beaker that contained potassium phosphate. The mixture was dissolved using the stirring rod‚ and then the magnetic stirring bar was placed in the beaker for further dissolve when measuring the pH. The pH meter was used to measure the solution
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Lab BCH2333 Section: 4 (Monday afternoon 1st week) Experiment 1: Carbohydrates Separation Techniques Based on Molecular Size Demonstrator: Date Preformed: January 26th‚ 2015 Date of Submission: February 2nd‚ 2015 Team #: Purpose The purpose of this experiment is essentially two-fold. Two of the four experimental procedures of this laboratory will be used to gain a conceptual and practical understanding of separation techniques used to isolate monomers from their respective polymers. Namely
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OIL SPILL PROJECT NO.6 Statement of Problem: In this project‚ the goal is to apply the knowledge of fluid dynamics in order to determine the effect of two parameters (oil density and volume fraction) on how long will it take for an oil drop to rise a distance of 1m in an oil/water distribution. Data: The following information was provided; | |Water | | | | | | | |
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expressed in Table 1. The methanolic fraction was more potent than the dichloromethane fraction‚ with an EC50 of 58.82µg/mL (50.65 – 68.31)‚ which was very similar with the standards‚ ascorbic acid and BHT‚ that exhibited an EC50 of 22.43µg/mL (16.07-31.30) and 40.55µg/mL (27.39 – 60.05)‚ respectively. By contrast‚ the EC50 obtained for the dichloromethane fraction was 344.70µg/mL (246.10 – 482.80) revealing to be six times less potent than the methanolic fraction. Regarding the TPC‚
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equilibrated with the 50mM NaOH buffer‚ pH 9 buffer was loaded on the column and elution was carried out using a 0-0.6M NaCl gradient [Kumar‚ H.1997]. 2.0ml fractions were collected and analyzed for proteolytic activity using BApNA and SAAPFpNA. Fraction was dialyzed against distilled water and concentrated for further analysis. Protein content of gut and fraction was determined by Lowry’s method [7]. Ammonium sulphate precipitated (60-90% saturation) and I. batata extract (200 TI units‚ 40mg proteins) were
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