Assessment By now you should have a good understanding of the scientific method and its importance in contributing to scientific knowledge. Grab your lab coat and get ready to design your own experiment. In this assignment‚ you will be doing two things: Evaluate an experiment. Designing your own experiment using the scientific method. Part I: Evaluate An Experiment Review each scenario below and choose ONE to complete for part I of your assignment. Read the scenario‚ and answer the
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Coli bacteria. We began the lab by first obtaining two sterile microcentrifuge tubes‚ one was label “GFP+” while the other one was “GFP–” because this one served as a control meaning nothing goes inside this one. With gloves on 25 microliters competent cells‚ which is e. Coli bacteria
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root tip of a plant spend a comparatively long time in the mitotic phase‚ whereas those cells comprising slow-growing tissues would spend most of their lives in interphase. Non-dividing cells remain in interphase and never enter the mitotic phase. (Lab Manual 64) Interphase is the synthesis of biological molecules including DNA and duplicated DNA with associated proteins. These comprise the chromatin that begin to condense toward the end of this phase‚ but are not yet visible. The nucleoplasm
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Isolation‚ Expression and Cloning of Lactate Dehydrogenase Gene from Lactobacillus bulgaricus Lactobacillus bulgaricus is used for industrial production of yogurt mainly because of his fermentative capability. In this experiment‚ the gene encoding the enzyme lactate dehydrogenase [ldhA] from the bactaria was isolated and cloned. The expressed ldhA gene was inserted into pET28b plasmid vector. The resulting recombinant pET28b-LdhA expression vector was then transformed after introduction into E.
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Results: The mealworms that were in the room temperature water have a higher cellular respiration rate than the mealworms in the cold water. This is because while they are in the cold water‚ the mealworm’s body slows down which decreases the amount of CO2 that is being produced. Unlike the mealworms in room temperature water‚ their cellular respiration was higher because their body temperature remained the same creating CO2 at a faster rate. Since there were two different controlled groups‚ there
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Introduction: Coccinellidae‚ which is more familiar known as the ladybug‚ comes from a family of small beetles. However‚ one may jump to conclusions after hearing their names‚ but ladybugs are not all females! Their color ranges from a red to a reddish-orange and even to a yellow color along with black spots on their hard-shell wing covers. Even though they are small bugs‚ they are very predatory. The ladybug’s mouth includes mandibles which is another word for their strong jaws (Hussain‚ et al
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Experiment 42: Ferrocene Preparation Introduction: The purpose of the lab is to prepare ferrocene from ferrous chloride and cyclopentadiene. Ferrocene‚ an organometallic‚ is a combination of two cyclopentadienide ions with a ferrous cation‚ such that the six pi electrons binds every carbon equally to the metal forming a sandwich type structure. Ferrocene has the properties of both an activated benzene (undergoes electrophilic substitution reactions) and a ferrous ion (oxidation reaction). Glyme
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human evolution. Although no humans are being observed in this lab the concept of evolution is going to be looked up. The speciation is to see if by different modifications in the environment create a new species of finches. If no new species develop or the finches do not evolve to the changes in their surroundings will natural selection happen and will we no longer have finches on this island? Materials * Computer * Evolution Lab * Internet Methods or Procedures (All changes are done
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The temperature of water was determined by a thermometer. 1 cm stripped ends of the wires was hooked into the input posts on the back of the impendence converter. Lab tutor module was started after. B. Baseline heart rate The top of the container was covered with foil and the animal was allowed to sit quietly for 10 min. The trace was examined by pressing start. After that the baseline or the controlled
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Polypeptides‚ better known as proteins‚ carry out nearly all of a cell’s functions. A protein is made up of at least one or more amino acid polymers. The necessity of proteins for life is unequivocal‚ not only do they help to form the structures of cells but they act as enzymes in reactions of the body1. Enzymes are biological catalysts that lower the amount of activation energy needed in carrying out biochemical reactions1. Enzymes are responsible for almost every reaction that occurs in a cell
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