Positive control – M. smeagmatis Blue – negative Pink /red- positive 4. Motility Positive control - P.vulgaris A. Non motile is negative test B. Motile is a positive test 5. Carbohydrates fermentation (test for gram -) ( glucose ‚ manitol‚ lactose) Positive control – E.coli (yellow) Red- no color change – negative test Yellow – color change – positive test 6. MaConkey’s Agar Positive control - E.coli Selective for negative gram stain . Differential for organism that could ferment lactase
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negative bacilli ‚ non-motile and capsulated . It appears as a mucoid in MacConkey agar it’s ferment lactose. According to biochemical tests the klebsiella gave negative result for oxidase‚ indole‚ methyl red‚ hydrogen sulfide‚ phenylalanine‚ gelatin hydrolysis‚ DNase and lipase. On other hand klebsiella gave positive result for citrate‚ urease hydrolysis‚ lysine decarboxalyze‚ acid form lactose‚ acid form glucose‚ acid form sucrose‚ acid form maltose‚ acid form mannitol and nitrate reduction. (1)
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reflected with an alkaline bi-product. The Enterotubes showed that Unknown #7 was an "enteric" bacterium with a score of "46141" Lysine‚ Adonitol‚ Lactose‚ Dulcitol/Phenylalanine‚ and Harnstoff/Uree were all negative‚ while Ornithine‚ Arabinose‚ and Sorbitol were proven to be positive. The IMViC test identifies members of Enterobacteriaceae and included Indole that tested negative‚ Methyl Red which produced a red color change showing that Unknown #7 could ferment glucose to acidic ends‚ Vogves Proskaer
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Identification of Bacterial Pathogens basic skills in diagnostic bacteriology Dr.T.V.Rao MD Dr.T.V.Rao MD 1 Identification of Microorganisms • For many students and professionals the most pressing topic in microbiology is how to identify unknown specimens. • Why is this important? • Labs can grow‚ isolate and identify most routinely encountered bacteria within 48 hrs of sampling. • The methods microbiologist use fall into three categories: ♣Phenotypic- morphology (micro and
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Microbial analysis of soil‚ of top layer from selected sites of Area near Dahisar River Saika N. Esani University of Mumbai (Email – saikae@ymail.com) Abstract: soil samples were collected fortnightly from area near Dahisar River‚ A river in suburb of Mumbai. laboratory analysis started from July 2010 to September 2010. Total bacterial and fungal count were estimated by standard spread plate isolation. Isolated bacteria were subject to colony characterization and were estimated by their
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growth and +1 to +4 for growth. Test E.coli K.pneumoniae S.epidermidis M.luteus Glucose Fermentation: acid gas + + + - Mineral salts plus glucose +2 +3 +3 +2 Lactose Fermentation: Acid gas + + + - Mineral salts plus lactose +2 +4 +3 +2 Sucrose Fermentation: acid gas - + + - Mineral salts plus sucrose +1 +4 +2 +2 Tryptophanase (Indole Test) + - - - Methyl Red Test + - + - Voges-Proskauer Test - + + - DISCUSSION 1. Oxygen Requirement E.coli was found facultative anaerobe because
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Lab Report 2 Biological Media Meva Tinsley Georgia State University Lab Report 2 Introduction: Biological Media Microorganisms require nutrients and certain environmental conditions in order to thrive. In the lab‚ we use a Biological Media to aid in growth reproduction. Also referred to as a culture medium‚ a Biological Media is a substance used to support the growth of microorganisms. The two types of media most commonly used in Microbiology are selective media and differential
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Introduction The science of microbiology is all about microorganisms‚ what they do and how they work. Microorganisms are ubiquitous‚ and are a very important part of life everywhere since they have been around for billions of years. The two main themes associated with microbiology are (1) having an understanding of the nature and function of the microbial world‚ and (2) being able to apply that understanding of the microbial world so that the benefits to the Earth and humans are known(6). This lab
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Introduction Water is necessary to life but it can be unhealthy too. In general‚ the water quality is poor in developing countries like Kenya. Water is a vector of diseases like yellow fever‚ diarrhea… Water purification is very important in these countries but people don’t have a lot of time to spare‚ they work or they are in the field and in general women must spend a significant amount of time walking to collect water. So we need to find a quick‚ easy‚ and economic solution that is as simple
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Protein Hydrolysis and Characterization Group 7 Niez‚ Robert Francis‚ *Orbin‚ Alfonso Ricardo* Parro‚ Athena Emmanuelle Peralta‚ Christian Department of Biological Sciences‚ University of Santo Tomas‚ Manila‚ Philippines • Abstract Hydrolyzed Protein is protein that has been hydrolyzed or broken down into its component amino acids. While there are many means of achieving this‚ two of the most common are prolonged boiling in a strong acid (acid-HVP) or strong base or using an enzyme such
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