broth will remain as liquid in ice bath. D) Utilization of amino acids Amino acids may be utilized to yield energy for the cells‚ for examples. NH3‚ indole‚ and H2O. - Indole Production Trytophan degrades to form indole. Only certain bacteria form indole. Thus‚ the degradation is suitable as another differentiating reaction. The presence of indole is tested using the Kovacs method. The reddening of alcohol layer indicates the
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Harley−Prescott: Laboratory Exercises in Microbiology‚ Fifth Edition Front Matter Preface © The McGraw−Hill Companies‚ 2002 PREFACE Take interest‚ I implore you‚ in those sacred dwellings which one designates by the expressive term: laboratories. Demand that they be multiplied‚ that they be adorned. These are the temples of the future—temples of well-being and of happiness. There it is that humanity grows greater‚ stronger‚ better. Louis Pasteur (French chemist‚ founder of microbiology
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Decolorization of Crystal violet dye by Aeromonas hydrophila isolated from textile wastewater Sujata Mani and Ram Naresh Bharagava* Laboratory of Bioremediation and Metagenomics Research (LBMR) Department of Environmental Microbiology (DEM)‚ School for Environmental Sciences (SES) Babasaheb Bhimrao Ambedkar University (A Central University) Vidya Vihar‚ Raebareli Road‚ Lucknow - 226 025 (U.P.)‚ India *Corresponding Author: Dr. R. N. Bharagava Tel.: (+91) 522-2998718; Fax: (+91)
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the bacteria colony then the bacteria is non-hemolytic. For my bacteria no change is observed in the media therefore the bacteria is non-hemolytic and negative result. MacConkey Agar test This test is used for isolating the lactose fermenting bacteria from lactose non-fermenting bacteria while inhibiting the growth of gram positive bacteria. The lack of growth from my bacteria indicates that my bacterium has negative result. Mannitol Salt Agar This test is used to observe the difference
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Examining the activity rate using DCIP and a spectrophotometer of succinate dehydrogenase isolated from Brassica oleracea mitochondria via mechanical disruption and differential centrifugation Introduction Mitochondria are important cellular organelles located in the cytosol of cells and is believed to have originated through an endosymbiotic relationship. The unique double layered membrane structure is responsible for the production of the primary energy currency of the cell; adenosine triphosphate
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5-nitro-3-[(5-nonsubstituted/methyl-4-thiazolidinone-2-ylidene)hydrazono]-1H-2indolinones (3a-j and 4a-h) was synthesized by cyclization of 5-nitro-1H-indole-2‚3-dione-3thiosemicarbazones (1a-k) with ethyl bromoacetate or ethyl 2-bromopropionate. The primary antiviral activities of the new hydrazonoindolinone derivatives‚ the previously reported 5-nitro1H-indole-2‚3-dione-3-thiosemicarbazones (1a-k) and 1-morpholino/piperidinomethyl-5-nitroindole-2‚3-dione-3-thiosemicarbazones (2a-l) were evaluated against
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was to do perform an Indole production experiment in order to cut my chances of finding my unknown in half. The Indole production test was recorded as negative. It is observed as negative because after adding the Kovac’s reagent there was no color change. Kovac’s reagent added to the tube reacts with DMABA and with any Indole present and produces a compound that turns the reagent layer pink. No color change for an Indole Production means tryptophan is not broken down into Indole and Pyruvate. After
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Digestion a. The mouth: salivary alpha enzyme chew food‚ perceive taste‚ moisten food with saliva‚ lubricate food with mucus‚ release starch –digesting (amylase) enzymes‚ initiate swallowing reflex - Enzyme: alpha amylase with cooked starch as substrate – starch digestion enzyme an enzyme that catalyses the breakdown of starch into sugar - The Functions of Saliva - 1. Moistens and lubricates food‚ permitting swallowing 2. Holds taste producing substances in solution and bring them in
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amino acids are transformed into phenol‚ cresol‚ indole and skatole • sulfur containing amino acids yield hydrogen sulfide‚ mercaptans and methane • some simple amino acids undergo deamination (removal of amine group) • others undergo decarboxylation (removal of carboxyl group) Examples: Tryptophan deamination Tryptophan -----> Indole propionic acid -----> Indole acetic acid -----> Skatole ----> Indole • Skatole and indole – responsible for the characteristic foul odor of
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Work Plan for Isolation‚ Purification‚ Identification and Starter Culture Activity of Lactoccocus lactis Submitted by: M.Usman Akram B.S. (Hons.) Dairy Technology mh.usman@hotmail.com Mobile : +923217773736 University of Veterinary and Animal Sciences‚ Ravi Campus Pattoki Lactoccocus lactis Classification: Scientific classification | Kingdom: | Bacteria | Division: | Firmicutes | Class: | Bacilli | Order: | Lactobacillales | Family: | Streptococcaceae | Genus:
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