products each year (“USDA ERS - Dairy Data‚” n.d.). When consumed the principle carbohydrate in dairy‚ lactose a disaccharide sugar‚ is either digested in the small intestine by lactase or is passed to the large intestine where it is broken down by bacteria. The lactose disaccharide is composed of the monosaccharides glucose and galactose. Lactase in an enzyme that catalyzes the breakdown of lactose into its two monosaccharides in a hydrolysis reaction so they can be absorbed through the intestinal
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test‚ distinguishing factors between lactose fermenters and non-lactose fermenters can be shown. If the organism applied to the agar produces orange colonies‚ and if the area around the bacteria turns orange as well‚ then the organism is a lactose fermenter. If the organism is not a lactose fermenter‚ the colonies will remain green or produce a blue color on the agar. Unknown #11 grew on the Hektoen enteric agar‚ giving evidence that the organism is a lactose fermenter. This evidence also suggests
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ique bacterial strains of E. coli by observing the proper responses of wild type E. coli Lac Operon as a control group. Mutated strains of E. coli were placed in four test tubes‚ each containing a different substance (lactose‚ glucose‚ water and lactose and glucose). After a warm water bath‚ Sarkosyl detergent‚ toluene and ONPG were added to the tubes and incubated again. The data was qualified by observing the color change from clear to yellow to indicate the presence of lactase
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FERMENTATION THROUGH DIFFERENT KINDS OF SUGARS In order to produce ethyl alcohol fermentation‚ we had to determine what sugars used‚ such as glucose (a single sugar) sucrose (table sugar) and lactose (milk sugar) will react with yeast to produce ethyl alcohol fermentation. My hypothesis is that the sugar lactose would produce the highest amount of bubbles and ferment. INTRODUCTION: The significance of fermentation is a lengthy process that has been practiced by humankind for thousands of years
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pass on their successful survival genes to the next generations. (M.Cummings) Lactose tolerance is a good example of natural selection. Lactose tolerance is the ability to digest milk and is a necessity for
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molecular basis of lactose intolerance. Students also learn about the scientific method by interpreting evidence to test hypotheses and designing the second and third experiments to answer specific scientific questions about lactase. 4. Review protein functions and reinforce the idea that‚ if a protein is missing or defective‚ this has observable effects on our bodies: Protein Function Examples Effect if This Protein Missing or Defective* Enzyme Lactase (breaks down lactose) Lactose intolerance
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EFFECT OF TURMERIC PASTE‚ LACTOSE AND NEEM PASTE IN THE CANCEROUS CELL IN MOUSE(STARTED 11/11/2011) (By DAY group—DEEPESH‚Anup and Yogesh MATERIALS * Turmeric paste * Lactose of milk * Neem paste REQUIREMENT * Three rats two cancerous one normal 2011/11/11 * ONIONS WERE KEPT IN WATER IN COUPLING JAR TO SEE IF OUR CHEMICALS AFFECTS THE CELL DIVISION OR NOT……. * ….E.coli was also inoculated but not used later * Neem leaf paste was prepaed in the mixture and kept
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Gene Expression Introduction: Escherichia coli are capable of using lactose as their sole carbon source. E. coli produces the enzyme β-galactosidase to digest the lactose into glucose and galactose. However‚ it would be inefficient to produce enzymes when there is no lactose available‚ or if there is a more readily-available energy source available such as glucose. Therefore there must be something controlling the expression of this enzyme. The purpose of the experiment is to determine whether
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unit 7.3 the experiment tested the ability of lactase to specifically bind and interact with lactose compared to maltose. In unit 7.4 the experiment tested the role‚ if any‚ that metal ions have on the activity of lactase. My hypothesis for unit 7.3 was knowing that lactase is specific for lactose‚ lactose will separate into galactose and glucose‚ as maltose will not change (153-155). Lactase should like lactose. For unit 7.4 my hypothesis was that EDTA will remove the ions‚ and will not work. Materials/Methods
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MacConkey (also McConkey) agar is a culture medium designed to grow Gram-negative bacteria and stain them for lactose fermentation. It contains bile salts (to inhibit most Grampositive bacteria‚ except Enterococcus and some species of Staphylococcus)‚ crystal violet dye (which also inhibits certain Gram-positive bacteria)‚ neutral red dye (which stains microbes fermenting lactose)‚ lactose and peptone. QUALITY CONTROL Results after 24 hrs at 35º C Organisms ATCC Growth Colour Escherichia
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