Abstract The experiment aims to observe if simpler substrates makes the rate of cellular respiration faster. Using yeast‚ smith fermentation tubes and different substrates namely‚ starch‚ lactose‚ sucrose‚ glucose and fructose‚ which are from different kinds of carbohydrates‚ ranging from the simplest sugars glucose and fructose to the polysaccharide starch and water as the control‚ the hypothesis was tested. With the span of thirty minutes with five-minute intervals‚ the height of carbon dioxide
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Write an explanation of how any two factors affect the rate of an enzyme-controlled reaction. How do these factors affect the chemical structure and properties of the enzyme. Many things can affect the rate of enzyme activity. The temperature of the enzyme‚ the pH of the solution‚ the concentration of the enzyme‚ substrate and the product. Also‚ another affector is the number of competitive and non-competitive inhibitors. As I cannot explain them all‚ I have chosen to explain the effect of temperature
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Cherno Okafor Aida Stefani SBI4U Octover 20th‚ 2012 Assignment 1: Cellular Biology furthers technology-Enzymes in the Dairy Industry Since ancient times‚ enzymes have played an important role in food production. Especially in the diary industry‚ some enzymes are required for the production of cheese‚ yogurt‚ and other dairy products‚ while others are used in a more specialized fashion such as improving texture or flavour of the product. Enzymes are used to catalyze the desirable reactions in industrial
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the negative control shows no activity as expected. Looking at the mutant‚ a large positive trend is seen in Miller Units overtime‚ more so than the positive control. Thus‚ the assumption can be confidently made the the mutant can in fact ferment lactose‚ which aligns with the MAC photos seen earlier. This also means that the mutation itself is likely not in the Lac-Z gene nor the promoter as both would prevent transcription and thereby production of beta-galactosidase. The gene that possess a mutation
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The aim of our investigation is to find out whether the use of different sugar isomers with yeast‚ will affect the rate at which the yeast respires at. The sugars to be tested are fructose‚ galactose‚ glucose‚ lactose and sucrose. Their effects on the respiration rate of yeast to be observed through the measurement (cm3) of the displaced water‚ which will tell us how much CO2 has been respired over 2 minutes. To compare the effects an average will be calculated for each and a result will be drawn
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Tests used Pre-enrichment- lactose broth Selective enrichment broth- with Tetrathionate Brilliant Broth Selective Plating- Brilliant Green Agar Isolation of salmonella conformation- is preformed using TSI and LIA Test results Tetrathionate Brilliant Green Broth (TGBG)was inoculated from the whirl pack spinach infected bag. TBGB inhibits the growth of organisms other than salmonella. The next test preformed was the Brilliant Green Agar (BGA)
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of the undesirable bacteria (Lee et al.‚ 1981). The media contain partially digested proteins which is known as peptone‚ that would dissolve in water and yeast extract‚ and also traces of Streptococci (Lee et al.‚ 1981). There is also maltose and lactose present which is served as the power house which will provide the energy for the growth of the bacteria (VanMeter and Hubert‚ 2013). Furthermore‚ sodium azide within the media is used as the selective agents that would allow only the growth of the
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Use of differential‚ selective and enriched media: EMB‚ blood and starch agar. OBJECTIVES: distinguish between different bacterial species based on colony morphology on agar plates To distinguish the growth characteristics of microorganisms in various differential‚ and selective media. Differentiate bacteria based on their ability to hydrolyze starch. Materials: Plates of EMB‚ Starch and blood agar. Stool sample. Inoculating loop. Bunsen burner. Soil sample. Cotton soap. Skin
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Tanti Lim Thurs AM Unknown Project I. Introduction The purpose to this lab was to isolate and identify two unknown bacteria from a mixed culture provided to us. This study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class. Each test performed‚ provided us with some key information about the unknown microbes in question . The identification of unknown bacteria is a time honored part of microbiology courses. It will challenge
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the rate of cellular respiration be measured to find out if sucrose‚ dextrose‚ lactose‚ or maltose lets the yeast to respire more than others. Hypothesis: I think‚ dextrose yeast solution will respire the most number of bubbles because it is the simplest sugar to break. Materials: * Knut * Dropper * Graduated cylinder (100ml) * Warm water * Yeast Solution * Sucrose * Dextrose * Lactose * Maltose Variables: Independent Variables: * The type of sugar Dependent
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