environment meaning more animals can stop their and release their feces‚ and also the animals that reside there continue to reproduce‚ leaving no exit for their excretions. By using the MPN test‚ I was able to detect the coliforms from the fermentation in lactose broths‚ then with the IMViC test I was able to identify the enteric bacteria colonizing in the water samples. By then end of the experiment‚ it was apparent that the USM Creek and Leaf River were the two that tested positive for possible
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unknown were not really helpful when it came to determining if it was Alcaligenes faecalis or Pseudomonas aeruginosa because they both had all the same characteristics when it came to the lactose‚ mannitol‚ motility‚ indole‚ citrate‚ H2S‚ urease‚ methyl red and VP tests. Our tests for lactose‚ mannitol‚ indole‚ urease‚ methyl
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OBJECTIVE: 1. To distinguish the bacteria abilities to metabolize various substrates and end products formed. 2. To observe the growth of different bacteria species in term of structures and its morphology based on different chemical substance applied. 3. To observe physiological and immunological properties utilized by different species of bacteria. INTRODUCTION: Bacteria biochemical testing can determine the types and numbers in terms of colony forming units of bacteria present in a
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unknown sample labeled number 15. The following are the tests that were used to identify the two different bacteria. The SIM test‚ which tests for sulfur reduction‚ indole production‚ and motility‚ MRVP which is a test used to distinguish different organisms within Enterobacteriaceae‚ the citrate test which is used to identify
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Online BIO 150 Introductory Microbiology #3 Lab Report NAME __ Lab Group 2_____ Answer the following questions as you work your way through the lab material typing in your answers. Then submit your finished lab report as a Microsoft Word document. This lab report is worth 100 points towards your final lab grade. Each Q is worth 2 points unless otherwise noted. Also‚ per the Honor Code‚ this work must be your own. This is due Mon. 10/8 at 11:59 PM. The theme of this lab is the identification
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production of the enzyme urease. McConkey agar contains lactose‚ which P.vulgaris does not ferment. It ferments glucose‚ sucrose‚ galactose‚ glycerol occasionally maltose with gas production‚ but never lactose. P.vulgaris ferments liquefies gelatin‚ casein‚ and blood serum‚ curdling milk with acid production. P.vulgaris provides a positive result for: sulfur reduction‚urease production‚ tryptophan deaminase production‚ and indole production. It’s not limited to any specific temperature
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(E.aerogenes)‚ Klebsiella pneumoniae (K.pneumoniae)‚ Proteus mirabilis(P.miranilis)‚ Pseudomonas aeruginosa(P.aeruginosa)‚ and Salmonella typhimurium (S.typhimurium). The biochemical tests utilized were; Triple Sugar Iron Agar (TSIA) test‚ Sulfur Indole Motility (SIM) test‚ Methyl red test and Voges-Proskauer (MR-VP) test‚ Citrate test‚ Urea test‚
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unknown was gram-negative‚ other bacteria’s were able to be eliminated from the options. The Triple Sugar Iron Test was chosen next because the unknown could be tested for glucose fermentation‚ lactose fermentation‚ sucrose fermentation and sulfur reduction. If the organism is able to ferment glucose and lactose and/or sucrose it will turn the medium yellow throughout (Leboffe & Burton 329). When the Triple Sugar Iron Test was complete‚ it showed a yellow butt‚ yellow slant and gas production. This determined
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gram-negative enteric bacteria. Mythelene blue inhibits gram-positive. The selective part is used for inhibiting gram-positive and has methylene blue. The differential part is what ferments lactose. MacConkey agar is a selective and differential media used to isolate gram-negative bacteria and differentiate between lactose fermenting and
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Red Broth. The first part of this test was for glucose fermentation with my results being +‚ which represented acid fermentation based on the yellow broth‚ and G‚ which represented gas end-products in the tube. The second part of the test was for lactose fermentation with my results coming back -‚ indicating that no fermentation occurred as a result of the red broth. The final part of the Phenol Red Broth tested for sucrose fermentation and my results were were + based on the yellow broth‚ which meant
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