of the undesirable bacteria (Lee et al.‚ 1981). The media contain partially digested proteins which is known as peptone‚ that would dissolve in water and yeast extract‚ and also traces of Streptococci (Lee et al.‚ 1981). There is also maltose and lactose present which is served as the power house which will provide the energy for the growth of the bacteria (VanMeter and Hubert‚ 2013). Furthermore‚ sodium azide within the media is used as the selective agents that would allow only the growth of the
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Use of differential‚ selective and enriched media: EMB‚ blood and starch agar. OBJECTIVES: distinguish between different bacterial species based on colony morphology on agar plates To distinguish the growth characteristics of microorganisms in various differential‚ and selective media. Differentiate bacteria based on their ability to hydrolyze starch. Materials: Plates of EMB‚ Starch and blood agar. Stool sample. Inoculating loop. Bunsen burner. Soil sample. Cotton soap. Skin
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Tanti Lim Thurs AM Unknown Project I. Introduction The purpose to this lab was to isolate and identify two unknown bacteria from a mixed culture provided to us. This study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class. Each test performed‚ provided us with some key information about the unknown microbes in question . The identification of unknown bacteria is a time honored part of microbiology courses. It will challenge
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the rate of cellular respiration be measured to find out if sucrose‚ dextrose‚ lactose‚ or maltose lets the yeast to respire more than others. Hypothesis: I think‚ dextrose yeast solution will respire the most number of bubbles because it is the simplest sugar to break. Materials: * Knut * Dropper * Graduated cylinder (100ml) * Warm water * Yeast Solution * Sucrose * Dextrose * Lactose * Maltose Variables: Independent Variables: * The type of sugar Dependent
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material from the cell. This test measures for the salt content in the solution. In the glucose test‚ Benedict’s test is performed using a 1:1 ratio of Benedict’s test to solution. These tests for the glucose in each solution. Finally the sucrose/lactose test uses the same content as the glucose test. Benedict’s test is performed using a 1:1 ratio of Benedict’s test to solution. This test measures for reducing sugars. The indicator for each test is color. When there is a color change to the solution
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Alyse Rose Microbiology Lab Bacterial Unknown March 25‚ 2013 Bacterial Unknown Report Each student was given an unknown bacteria to figure out. I was given the unknown bacteria S38. Everybody is supposed to do all sorts of test to identify the bacteria. The first thing I did was smear my bacteria on a liquid medium. I then proceeded to incubate the medium for 24-48 hours. 1. GRAM STAIN The next step I took in finding my unknown bacteria was to gram stain it. This is used to differentiate
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for gram positive‚ since unknown number five was determined to be gram positive rod. The other tests were performed in this order: Mannitol Salt (MSA) streak‚ Blood Agar streak‚ Catalase test‚ Nitrate Reduction test‚ and Phenyl Red Broth test for lactose and sucrose fermentation. After performing a gram stain on unknown number five‚ number five was determined to be gram positive rod. On the TSA plate‚ number five had the following morphology: very large‚ raised‚ opaque cream color colonies that
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test results were negative. An EMB plate of the bacteria was examined to identify whether the bacterium was S. typhi or E. coli. The growth on the EMB plate was shiny and metallic green‚ meaning that it was positive for lactose fermentation. S. typhi exhibits intermediate lactose fermentation with pink or purple growth on an EMB plate‚ so it was eliminated as the possible identity of Bacteria A. To confirm the identity of Bacteria A as E. coli‚ an Enterotube test inoculated with Bacteria A was observed
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Six substrates were used. They were the following with the volume of carbon dioxide they produced in cm3. Sucrose –34.35‚ fructose –33.84‚ glucose –31.81‚ starch –1.57‚ and both lactose and distilled water got zero. Some substrates have simple compositions and are easy to break down unlike while some have complex compositions and are hard to break down during the process of respiration. With the conditions that some sugars still
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In order to explain how my genetics and family medical history impacts me‚ I must first briefly explain the disease that is the risk factor for me. Celiac disease is a hereditary immune system-mediated disorder that can lead to other serious illnesses such as cancer. Celiac disease affects the small intestine and many other parts of a person’s body; Celiac disease is a disease which makes a person unable to ingest gluten because of the affects that gluten has on the body. Gluten is a protein found
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