tests was to differentiate microbes from one another and to compare how metabolic and biochemical processes differ from species to species. The tests performed include: the Fermentation of Sugars Test (sucrose‚ glucose‚ and lactose)‚ the Urease Test‚ the Fermentation of Lactose Test‚ the Sulfide Indole Mobility (SIM) Test‚ the Nitrate Reduction Test‚ the Protein Hydrolysis Test‚ the Catalase Test‚ and the Cytochrome Oxidase Test. The microbes that were tested during this lab were: Escherichia coli
Premium
Beta-galactosidase is an enzyme that initiates the breakdown of the substrate lactose. Lactose is a disaccharide sugar found in milk. It is composed of two rings. The rings contain 5 carbon atoms and an oxygen atom. Attached to the carbon atoms are a hydrogen atom and an OH group or another carbon. The rings are bound together by an oxygen bridge. This bridge can be broken when the enzyme Beta-galactosidase binds to lactose and a water molecule reacts with the oxygen atom in the bridge. (www.biology
Premium Enzyme Chemical reaction Catalysis
altered during the process. The enzyme used in this experiment was the β-galactosidase purified from E. coli. This enzyme hydrolyzes lactose and turns it into galactose and glucose. Since it is difficult to assay the activity of β-galactosidase‚ we will be using the artificial substrate‚ o-nitrophenyl-β-galactoside (ONPG) instead of lactose. ONPG is an analog of lactose and an advantage of using ONPG is that it is easy to determine the amount of ONPG cleaved by using spectrometric assay (1). The β-galactosidase
Premium Enzyme Chemical reaction Catalysis
dehydrogenase is very stable and is found at high concentration only in the mitochondrion of eukaryotes. It can be easily used as a marker enzyme for mitochondria and we used it to estimate the realative amounts of mitochondira in…Jacob and Monod used the lactose metabolism system of Escherichia Coli (E. Coli) to better understand the problem of enzyme induction‚ the occurrence of a particular enzyme only when its specific substrate is present. In the lac operon system‚ enzyme induction occurs when the presence
Premium
producing lactase (the enzyme to break down lactose) as we grow into adults. BUT the Masai and Loetschental Swiss civilizations‚ who are free of modern‚ degenerative diseases‚ receive a large percentage of calories from dairy products. (Chris Kresser) In areas of the world where dairy has been consumed for thousands of years‚ it seems that people’s genes have been mutated or developed in order to digest lactose better. (1) Your ability to digest lactose could very well depend on your ancestors. The
Premium Nutrition Obesity Health
identification tables and dichotomous keys that were given in the lab manual. Since unknown A was determined to be a Gram positive rod and an endospore producer‚ the genus Bacillus was then concluded. Unknown A bacteria was then inoculated into a lactose fermentation broth tube and a mannitol fermentation broth tube for further testing of the species. Note‚ all of these tests were performed by the methods listed in the lab manual (171-173). All of the following biochemical tests were perform on
Premium Bacteria Microbiology Gram staining
be examined‚ by measuring the pH of reaction‚ lower pH means more CO2 production. The first factor being measured is the type of sugar used in the fermentation of yeast. The types of sugar being used in the experiment is glucose‚ sucrose‚ and lactose. Glucose is a monosaccharide and is used in the first step of glycolysis in order to help create pyruvates‚ which are then used to create ethanol as long as there is no mitochondria or oxygen present. Sucrose is a disaccharide‚ commonly referred to
Premium Glucose PH Yeast
started the protocol for the lactose test2 which would help me determine which test to conduct next to narrow down list of possible bacteria. I conducted the protocol for MR test3 and citrate test4 the same day as the lactose test to speed up the process of elimination. With conducting the MR. Citrate and Lactose test all in the same day‚ I learned that time management was a big deal and I learned quick and caught up with the class. The next class I observed my lactose tube and my results were AG
Premium Bacteria Microbiology Escherichia coli
include: * Body weight; being underweight‚ overweight‚ a healthy weight‚ obese. * Birth weight; being underweight is a huge risk for babies. * Genetics; sex‚ cancers‚ allergies. * Glucose regulation; also referred to as glucose intolerance or insulin resistance. * Blood pressure; can lead to a lot of heart problems such as a stroke. * Blood cholesterol; if build up is extreme it can lead to brain damage The determinants above are varied and although most people have some
Premium Obesity Cancer Hypertension
When it comes to keeping the human body‚ nutrition is the most essential part of everyone’s life. If people do not have full control‚ it will affect the way they will be in the future. Any change to someone’s diet will change their body in the long run‚ whether it be positive or negative. It is quite apparent‚ especially in America‚ that the common person’s nutrition has gone down hill. Since the 1980s‚ the rate of obesity has inflated double the amount for adults and triple for children (“Obesity”
Premium Nutrition