Intro: Carbohydrate‚ any of a large group of compounds in which hydrogen and oxygen‚ in the proportions in which they exist in water‚ are combined with carbon; the formula of most of these compounds may be expressed as Cn(H2O)n. Structurally‚ however‚ these compounds are not hydrates of carbon‚ as the formula would seem to indicate. Carbohydrates‚ as a class‚ are the most abundant organic compounds found in nature. They are produced by green plants and by bacteria using the process known as photosynthesis
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DNA Extraction Lab Problem Statement: Do you think you have ever eaten DNA? Background Information: DNA is too small to see under a regular microscope‚ so how can it be studied? DNA is a large molecule found in all living things; therefore it is possible to extract it from cells or tissues. All we need to do is disrupt the cell’s plasma membrane and nuclear envelope‚ make the DNA clump together and - voila! - DNA extraction is possible. DNA extractions from onion‚ bananas‚ liver‚ or wheat
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Enzyme Lab Experiments Problem: How can we demonstrate how enzymes work? What happens if we alter the environment of an enzyme? Materials: G;lucose Test Strips Test Tubes Pipettes Raw Hamburg Lettuce Potato Raw Liver Chalk Beakers Dairy Lactose Tablet Water Sugar Solo Cups Hot Plate Knife Gloves Skim Milk Glow Sticks Peroxide Hypothesis: 1. If we change the environment via temperature the glow stick will Its intensity will change 2. If hydrogen peroxide is added to a certain food liver
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DISACCHARIDES (Haworth and Chair structures only) Lactose Sucrose HAWORTH CONFORMATION HAWORTH CONFORMATION CHAIR CONFORMATION CHAIR CONFORMATION Maltose HAWORTH CONFORMATION CHAIR CONFORMATION POLYSACCHARIDES Amylose CHAIR CONFORMATION HAWORTH CONFORMATION Glycogen B. BENEDICT’S TEST FOR REDUCING SUGARS Compound Color Reducing Sugar (yes/no) Water Blue No Glucose Red Yes Fructose Reddish-Orange Yes Sucrose Blue No Lactose Red Yes Maltose Red Yes Starch Blue No Glycogen
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staining method. Carbohydrate fermentation: 1) Phenol red lactose broth. 2) Phenol Red Glucose broth. 1) Phenol red lactose broth test This test is used in order to indicate whether the organism can use lactose as the source of energy or not. Phenol red indicator also added in the broth. In this experiment the color remained red and didn’t change which makes the test negative. It means that the organism didn’t ferment with phenol red lactose (PRL). 2) Phenol Red Glucose broth test In this test
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In specific‚ how the type of sugar affects the rate of fermentation. The aim of this experiment was to find which type of sugar was best suited to produce ethanol. In the experiment‚ four different sugars were used‚ they included sucrose‚ glucose‚ lactose and fructose. This research is still relevant today‚ as alcohol is still consumed and is required on a large scale. This makes it crucial to companies to find the best sugar to use in the fermentation process. Cellular respiration is a process that
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intracellular inverters or sucrose synthase Lactose A sugar present in milk. It is a disaccharide containing glucose and galactose units. Lactose is milk sugar. It’s not as sweet as table sugar‚ but like table sugar‚ lactose provides your cells with energy when you eat it. You can use lactose for a number of other purposes‚ all of which it has in common with other carbohydrates like table sugar and starch. Because you can’t absorb whole lactose‚ your cells can’t use it. But they can use
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positive (+) reactions or negative (‐) reactions to fructose‚ sucrose‚ cellulose‚ starch‚ lactose and an unknown carbohydrate. Observations and Results Report your test results in a tabular format • Record the bacteria identified in each of the Four Media Tests. Test Bacterium 1 Test Bacterium 2 Test Bacterium 3 Test Bacterium 4 Gram Stain Gas from Glucose Acid from Glucose Sucrose Lactose Identification Initial color of medium in Gas and Acid from Glucose test: If you
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can distinguish isomers of the same molecule. The enzyme invertase specifically catalyzes the reaction of the conversion of sucrose to its individual carbohydrates glucose and fructose. It does not catalyse the reaction of maltose to 2 glucose or lactose to galactose. In this experiment‚ titrimetric and spectrophotometric methods were used to determine the specificity of invertase by determining the amount of glucose converted from the given disaccharides. The results show that sucrose yielded the
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no growth indicated that the bacteria grown was gram-positive. Whereas no color change inferred that the bacteria grown can’t ferment lactose whereas color change to pink inferred that the bacteria grown can ferment lactose. The results acquired by our test was positive with color change to pink. Hence the unknown bacteria was gram-negative and can ferment lactose. Amongst Escherichia coli and Proteus vulgaris‚ the result MacConkey agar confirmed that the unknown bacteria was Escherichia
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