Use of SDS-PAGE/Western Blot Techniques as Diagnostic Tools for Equine Medical Conditions SDS-PAGE Overview Electrophoresis is commonly used to separate proteins using an applied electrical field. SDS-PAGE separates proteins by molecular weight‚ using sodium dodecyl sulfate (SDS; also known as lauryl sulfate) to denature them and a discontinuous polyacrylamide gel as a sieving matrix. The net charges of folded proteins are not dependent upon molecular weight. Rather‚ charge is determined by
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8. Calculating Interest Rates. In 2010 the average price per metre for owner-occupied flats in Copenhagen was about 23.000 Danish kroner. In 1995 the average price was around 6.000 Danish kroner. What was the annual increase in selling price? t = 2010 – 1995 = 16 FV= PV * (1 + r)^t 23.000 = 6000 * (1 + r)^16 (1 + r)^16 = 23.000/6000 = 3‚83 1 + r = 3.83^(1/16) = 1.087552 1.087552 – 1 = 0.087552 = 0‚088 r = 8.8% 9. Calculating the Number of Periods. You’re trying to save to buy a new
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Ernest Hemingway is an author well known for the common themes in his novels. In his style of writing‚ Hemingway is able to express the themes of the novel through strong character traits and actions. The common themes in Hemingway ’s novel The Sun Also and A Farewell to Arms are death and loss. The characters in these novels‚ and many of Hemingway ’s other novels‚ can relate to these themes. The novels The Sun Also Rises and A Farewell to Arms share many similarities. These of course include the
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In this passage of Things Fall Apart‚ by Chinua Achebe‚ the protagonist and main characters of the novel‚ Okonkwo‚ has just been sent into exile away from his land of Umuofia ‚ Nigeria . The crime he has committed to receive such a punishment is the sin of murder. While he is attending a funeral for a man named‚ Ogbuefi Ezeudu‚ Okonkwo’s gun accidentally explodes and kills Ezeudu’s sixteen-year-old son. Killing a clansman is a crime against their earth goddess‚ thus‚ Okonkwo and his family are sent
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The last drop of sweat my body has to offer slowly falls from my forehead onto my two-year-old neatly pressed black concert dress. In the first oboist’s chair I sit‚ horn neatly propped in my lap‚ as I wait for the conductor to walk onto stage. My slightly trembling hands make physical the instinct to run‚ hide‚ offer some excuse and exit stage left. My mind flashes to the earlier part of this day‚ filled with practice and reed-making‚ in preparation for the moments about to unfold. Doubt‚ worry
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Edgar Allan Poe Research Paper The topic of this research paper is to attempt to explain the writing styles and writing techniques used by of one of the most famous American short-story writer‚ author‚ and poet‚ Edgar Allan Poe. This paper will also discuss why when Poe writes most of his short stories or poems‚ he chooses to write them in the first person point of view of the narrator or main character of the story. It will discuss that Poe chooses to write like this because it allows the reader
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The Effectiveness of Facebook Advertising or Fan/Like Pages A study of Young Adults over the ages of 13 By Joe De Wet (197031803) Laura Leigh Poonsamy (208159517) Theodore Zeederberg (2080230416) Assignment 1 submitted in partial fulfilment of the requirements for the diploma National Diploma: Marketing in the Faculty of Business at the Cape Peninsula University of Technology Lecturer: Mr RG Duffett Cape Town 9 May 2011 DECLARATION I‚ Joe De Wet‚ declare that
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How Does Sheriff present human weakness to create tension within the pages 54 to 59? ‘Journeys End’ by R.C. Sheriff is a World War One play following the life of Commander Stanhope and his men and how the war affects them. It was first performed in 1928 and Sheriff’s use of realism shocked the contemporary audience. Sheriff’s aim‚ I think‚ was to educate the audience on the events of the war and especially its affects on soldiers as there is very little actual violence on stage‚ so it is left
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This case focuses on total quality management analysis of the successful new initiatives of AT&T Universal Card Services. It describes the theory and operations of the AT&T Universal card‚ a credit card introduced successfully in 1990. Specifically‚ the case looks at UCS’s methods of motivating and monitoring its frontline telephone associates‚ those who deal with individual customers and who‚ the firm hoped‚ would succeed in not just serving but "delighting" customers. AT&T’s has two main goals:
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In this week’s assignment I will attempt complete exercises 35 and 37 in the “Real World Applications” section on page 280 of Mathematics in Our World. For each exercise‚ specify whether it involves an arithmetic sequence or a geometric sequence and use the proper formulas where applicable. I will try to format my math work as shown in the “week one assignment guide” provided to us and try to be concise in my reasoning. Exercise 35: A person hired to build a CB Radio tower. The firm charges
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