Determining the Catalytic Properties of the Enzyme Peroxidase Extracted from a Turnip Under the Conditions of Temperature‚ pH‚ Boiling and Competitive Inhibitors By Robin Caserta BIO 101 September 30‚ 2013 ABSTRACT The enzyme‚ peroxidase‚ extracted from a turnip was tested for its efficiency in binding to its substrate and its stability under several conditions. To do this‚ we tested effects on peroxidase activity‚ first‚ with different amounts of the enzyme‚ next at temperatures of
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Title: Enzyme Catalysis of Hydrogen Peroxide by Catalase Problem and Objectives: How do different temperatures and different levels of pH affect the reaction rate of the enzymes in chicken liver? Demonstrate the activity of an enzyme in living tissue‚ observe the effects of changes in temperature and pH on the activity of an enzyme‚ perform analyses for the presence of an enzyme in tissues‚ and analyzing relationships between environmental conditions and enzyme activity. Background: Cells produce
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Halobacterium Lab Investigation Mason Pirio 12/12/13 Period 5 Table Group 3 PS 2- Experiment Design PS 3- Conclusion Writing PS 13- Nitrogen Cycle Abstract- In this experiment we tested to see how well halobacterium grows in different levels of salinity. We found out that the higher salinity in the growth medium the better the halobacterium grows. Background Information- Halobacterium is a bacteria that is found in the great salt lake. Halobacterium is an extremeophile which means it thrives
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Effects of Temperature on the Formation of Cheese Curds This lab was performed in order to find what temperature the enzyme chymosin is most effective at forming cheese curds. An enzyme is a protein that acts as a catalyst to speed up biological reactions in cells. They work by lowering the activation energy and help to orient the molecules correctly. Chymosin was originally taken from the stomachs of calves‚ and was called rennet‚ and then purified to make rennin. Eventually to catch up with
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It was hypothesized that if the animal was larger‚ then it would have larger hemoglobin. It was thought that larger mammals would need more oxygen-rich hemoglobin to support healthy function. This lab did not support this hypothesis. The results in table 1 displayed that the hemoglobin of all mammalian samples traveled about the same distance. This trend can be seen in graph 1 as the mammalian hemoglobin samples migrated the same distance. Because the proteins traveled around the same distance it
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The main purpose of this lab is to explain what emulsifiers and emulsions are and how emulsifiers affect emulsion stability. An emulsion is a mix of to or more liquids that usually do not mix together. One liquid is evenly dispersed in small droplets throughout the other liquid. Many emulsions contain water as one of the phases. There are two different types of emulsions. One type of emulsion contains oil droplets that are dispersed within water. It is considered oil in water emulsion. The other
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iron powder. Para-aminophenol crystallises out from the mother liquid which is subsequently centrifuged. Para-aminophenyl crystal is then reacted with acetic anhydride to produce pure paracetamol. The reactions are summarised as shown below: Lab scale manufacture Paracetamol can be easily prepared in the laboratory using phenol. Phenol reacts with sodium nitrate and separate para nitro phenol from outho nitro phenol. Then para nitro phenol is converted into para amino phenol using sodium
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Assessment By now you should have a good understanding of the scientific method and its importance in contributing to scientific knowledge. Grab your lab coat and get ready to design your own experiment. In this assignment‚ you will be doing two things: Evaluate an experiment. Designing your own experiment using the scientific method. Part I: Evaluate An Experiment Review each scenario below and choose ONE to complete for part I of your assignment. Read the scenario‚ and answer the
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Coli bacteria. We began the lab by first obtaining two sterile microcentrifuge tubes‚ one was label “GFP+” while the other one was “GFP–” because this one served as a control meaning nothing goes inside this one. With gloves on 25 microliters competent cells‚ which is e. Coli bacteria
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root tip of a plant spend a comparatively long time in the mitotic phase‚ whereas those cells comprising slow-growing tissues would spend most of their lives in interphase. Non-dividing cells remain in interphase and never enter the mitotic phase. (Lab Manual 64) Interphase is the synthesis of biological molecules including DNA and duplicated DNA with associated proteins. These comprise the chromatin that begin to condense toward the end of this phase‚ but are not yet visible. The nucleoplasm
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