3.6.5. Southern hybridization Southern blot hybridization analysis was carried out using standard procedures (Sambrook et al. 2001). Approximately 20 µg of total genomic DNA from transgenic plants and untransformed control plant was digested with EcoRI‚ and then separated by electrophoresis on 0.8% agarose gel. Digestion profile was checked with 1µg DNA‚ with single cutter enzymes EcoRI (10U) in T-DNA region. For southern experiment‚ 20µg DNA was digested overnight. Restricted fragments were separated
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Southern Pulp and Paper Introduction In this work I am going to identify the Southern Pulp and Paper’s Toccoa mill problems and issues that are available to mill’s manager Curtis Shelton. Part 1 In considering the overall process on the pulp and bleached products industry it is always good to start with the main performance objectives and measures. One of the first important issues is the process design and layout. How the process architecture looks like and how well it is designed
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The Truth of a Southern Dream In Fitzgerald’s "Ice Palace"‚ Sally Carrol Happer is a Georgia girl tired of the lazy and unambitious South. She desires to break free from her hometown of Tarleton‚ captivated by the promises and new opportunities in the North. Her fiancée Harry is supposed to be the fiancée that can provide her this new lifestyle‚ but her romanticized vision becomes disillusioned. Sally Carrol’s misconception of the shimmering North is captured after experiencing the reality of
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The American colonies differed greatly from one another. Although they were in the same region of the land‚ some had better farming than that of others and some had better trade routes than the other colonies. No two colonies had the same agricultural needs. Georgia’s agriculture is different from Rhode Island. They also differed by the use and need of slavery. Slaves are needed more in agricultural areas than trade areas. That doesn’t mean that the northern colonies did not have slaves; it just
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In the fifth chapter‚ this thesis will assess the abolitionist effort to denounce the legitimacy of using the Bible to sanction southern slavery by arguing that biblical slavery was not based upon the inferiority of one race whereas southern slavery was based upon the inferiority of one race. In short‚ these abolitionists sought to highlight that southerners were using a book which sanctioned a system of slavery that was not based upon the inferiority of one race to sanction a system of slavery that
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Find‚” are perfect examples of Southern Gothic literature. Southern Gothic literature has points that must be satisfied in order to be classified in this genre. “A Rose for Emily” and “A Good Man is Hard to Find” satisfy all of these requirements. These requirements include setting in the south‚ puzzling characters‚ and exposing the deeper issue behind the face value of problems. These two short stories combine these three elements to produce two pieces of Southern Gothic literature. “A Rose for
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Cited: Wells‚ Ida B. Southern horrors and other writings : the anti-lynching campaign of Ida B. Wells‚ 1892-1900. Edited and with an introduction by Jacqueline Jones Royster. Boston : Bedford Books‚ 1997.
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deserved. The English believed that the best way to go about this was to colonize the New World. Subsequently‚ many colonies began to develop‚ and of these colonies‚ Massachusetts and Virginia were the most well-known. The early settlements of the Massachusetts and Virginia were both established by similar groups of people at the same time; however‚ their contrasting beginnings as a colony‚ views on religion‚ and means of economic stability created two different politics and economic systems. The settlements
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Race notes-sep.18 * Southern slavery * Age of flexibility (1619-1680) * South Carolina Slave Majority * Slave codes -status of the mother -chattel slavery-slaves are not even people‚ no rights -miscegenation After the revolutionary war: Economics- economy based on agriculture in south‚ so slaves are key Land expansion Property rights Scientific racism * carl Linnaeus Haitian revolution (1791-1804) scares americans -Toussaint
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The Causes for Colony Collapse Disorder Bees play an integral part in daily human life. Bees pollinate $14 billion in US crops per year‚ which includes apples‚ coffee‚ and almonds (Danforth‚ 2007). Unfortunately‚ they have gone through a rapid decline in population in recent years; managed honey bee population has decreased by one-fourth in Europe between 1985 and 2005‚ and by more than one-half in North America between 1947 and 2005 (Christen‚ Fent‚ & Mittner‚ 2016). The economic value behind bees
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