of acid-base titration‚ an appropriate indicator must be chosen. However‚ the end point can also be determined potentiometrically using a pH meter or by a conductometric method. At this point‚ all the acid has been neutralized and neither excess base nor excess acid is present in the solution. The solution consists of salt and water only. That is why acid-base titrations are also called neutralization titrations (Sienko and Plane 1957‚ 340-343). Neutralization reactions in experiments: NaOH(aq)
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the first step is converted to S-adenosyl-methionine (AdoMet) by AdoMet synthetase3‚4. The 1-Aminocyclopropane-1-Carboxylic acid Synthase (ACS) catalyzes the conversion of AdoMet to 1-aminocyclopropane-1-carboxylic acid (ACC)5‚ which is the rate-limiting step in ethylene biosynthesis. ACC is then converted to ethylene by ACC oxidase (ACO)‚ a member of the oxygenase/oxidase superfamily of enzymes6.The ACS plays a central role to regulate ethylene production through changes in ACS gene expression levels
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Neurophysiology Lab Report Anatomy & Physiology Lab Report Exercise 3 Activities 1-4‚ 8 By Laurence Blake 2/27/12 A. Objective I. Activity 1-4: Eliciting a Nerve Impulse • Investigate what kinds of stimuli stimulate action potential. II. Activity 8: Nerve Conduction Velocity • Determine and compare the conduction velocities of different types of nerves. B. Introduction I. Activity 1-4: Eliciting a Nerve Impulse • In this experiment‚ we
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Engineering B45 Concrete Lab Report Introduction: Concrete is a mixture of sand and rock or similar inert material (aggregates) held together by a cementing material. Usually the cementing material is Portland cement‚ but sometimes binders such as asphalt or gypsum are used‚ in which case the concrete may be called asphaltic concrete or gypsum concrete. Properties of concrete are governed not only by the properties of its ingredients (cement‚ water‚ sand‚ and coarse aggregate) but also‚ to a great
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is unlimited per the lab manual.(Bluedoor) If there is no competition in the water‚ the growth can be unlimited. The population will have favorable
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Lab report NF-κB 1. Aim The aim with this lab was through using ELISA-based technique learn how IL-1 and LPS activate the NF-κB signaling pathway in insulin-producing cells and see if the activation can be prevented through inhibition of proteasomal activity. In addition the purpose of this lab was to detect whether NF-κB activation leads to induction of the iNOS enzyme or not. 2. Background NF-κB is a transcription factor that in its inactive form is bound to IκB‚ which is a cytoplasmic inhibitory
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allow an object to move is dissipated into heat energy and will not return to the system once the movement stops. Specifically‚ this lab will calculate the coefficient of friction. Unlike most coefficients in Physics‚ friction behaves differently depending on whether the object is at rest or at motion.
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| | Kinetics Author: Katie Wood Instructor: Donald Kavanagh Chem 106b‚ Section 001 Lab Performed 8th‚ 2012 Lab Report Submitted February 22nd‚ 2012 Abstract The purpose of the lab was to determine the order of reaction for the dye Red #40. By measuring the reaction rate between bleach and the dye‚ the order of the reaction was determined to be first order. Introduction The study of kinetics is important for studying the amount of time it takes for a particular reaction to reach
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9/23/12 Lab Report #1 Meter Reading Summary The objective of this experiment was to learn how to read different meters like the D.C. volt meter and the D.C. amperes meter. In all meters each big line is a major division and each little line in between is a minor division‚ and if there is a line smaller than the minor division lines then that would be a sub minor division. Each meter has a low‚ medium‚ and high range. For example on the D.C. volt meter the ranges go from top to bottom 150‚ 15‚
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Chelex beads and 100µl of the supernatant was transferred to a fresh eppendorf tube. In order to access the purity and concentration of the DNA sample‚ the NanoDrop 2000 was used with the accompanying Nanodrop 2000/2000c operating software. Reagents Amount(µl) Diluted template DNA stock (DNA diluted using TEA buffer to obtain a concentration of 10ng/µl) 2.50 Taq 2x Master Mix 12.50 Amelogenin Forward Primer(10µM) [primer sequence: GCTCTGATGGTTGGCC]
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