Investigating the Effect of Lipase Concentration on the Breakdown of Fat in Milk INTRODUCTION: Enzymes are proteins which can catalyse chemical reactions without changing themselves. The enzyme lipase breaks down the fat in dairy products such as full-cream milk for people who are lactose intolerant. Lipase acts on its specific substrate‚ lipids produces fatty acids. If enzyme concentration increases‚ random collisions between the substrates and active sites of enzyme increase due to the increasing
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2002‚ 7: 57-66 Hydrolysis of Oils by Using Immobilized Lipase Enzyme: A Review V Ramachandra Murty*‚ Jayadev Bhat‚ and P. K. A. Muniswaran . Department of Chemical Engineering‚ Manipal Institute of Technology‚ A Constituent Institution of Manipal Academy of Higher Education (Deemed University)‚ Manipal-576119‚ India Abstract This review focuses on the use of immobilized lipase technology for the hydrolysis of oils. The importance of lipase catalyzed fat splitting process‚ the various immobilization
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diastase‚ pepsin‚ lipase‚ catalyse and urease. Lipase is an enzyme found in the digestive tract that catalyses the break down of fats into individual fatty acids that can be absorbed into the bloodstream. This enzyme will be used in this EEI. The purpose of this EEI is to test the rate in which lipase reacts to different variables such as temperature and pH. Enzymes are found in foods‚ epically raw foods such as meat‚ but cooking foods destroys any enzyme activity‚ including lipases. If the temperature
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LAB 9: DATE: 25TH January‚ 2011. FORM CLASS: L6 3 SUBJECT: Biology TITLE: Enzymes AIM: To investigate the effect of temperature on the enzyme lipase INTRODUCTION: The phenomenon of catalysis makes possible biochemical reactions necessary for all life processes. Catalysis is defined as the acceleration of a chemical reaction by some substance which itself undergoes no permanent chemical change. The catalysts of biochemical reactions are enzymes and are responsible for bringing about almost
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AIM The aim of this investigation is to explore the effect of different concentrations of bile salts on the time taken for the lipase enzyme to break down fat. BILE Bile is a brownish bitter alkaline fluid produced by the liver and made by the hepatocytes from water‚ bile salts‚ bile pigments cholesterol and phospholipids and stored in the gall bladder. Bile is directly connected with digestion. It is released sporadically into the small intestine (duodenum) which is part of the gut in order
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The lipase gene sequence from Alcaligenes sp. JG3 was translated into amino acid sequence using two methods‚ manual translation and ExPASy online software. By manual method‚ amino acid sequence was obtained via codon translation one by one as shown in Figure IV.7. The sequence reference used is the lipase from A. faecalis MOR02 due to having high similarity with the lipase sequence from strain JG3 during CLUSTAL alignment and the alignment of the deduced amino acid with the referred sequence was
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Research Question Does the concentration of substrate (H2O2 (hydrogen peroxide)) have an effect on the activity of the enzyme (catalase)? Theory The higher the substrate concentration the more quickly product is produced (rate of reaction increases) until enzyme saturation is reached at which time more substrate has no further effect. Enzymes such as Catalase are protein molecules which are found in living cells. They are used to speed up specific reactions in the cells. They are all
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Packed and Loose milk” This research paper is all about the awareness and acceptance of Packed and Loose milk by the people. Milk is an opaque white liquid produced by the mammary glands ofmammals It provides the primary source of nutrition young mammals before they are able to digest other types of food. The early lactation milk is known ascolostrum‚ and carries the mother ’s antibodies to the baby. It can reduce the risk of many diseases in the baby. The exact components of raw milk varies by species
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Everyone has heard the saying “drinking milk helps build strong bones”‚ but is this really true? According to Walter C. Willett‚ chairman of the Department of Nutrition at the Harvard School of Public Health‚ “There’s no solid evidence that merely increasing the amount of milk in your diet will protect you from breaking a hip or wrist or crushing a backbone in later years” In his studies he found that women who drank milk twice a day were as likely to suffer bone breaks as women who drank it once
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How does temperature affect the rate of reaction for Lipase? As the temperature increases‚ so will the rate of enzyme reaction. However‚ as the temperature exceeds the optimum the rate of reaction will decrease. I predict that at temperatures above 70°C the enzyme lipase will become denatured and at temperatures below 10°C the enzyme will become inactive. Since lipase operates within the human body I’d also predict that its optimum temperature would be around human body temperature which is approximately
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