------------------------------------------------- Liquid–liquid extraction From Wikipedia‚ the free encyclopedia Liquid–liquid extraction also known as solvent extraction and partitioning‚ is a method to separate compounds based on their relative solubilities in two different immiscible liquids‚ usually water and an organic solvent. It is an extraction of a substance from one liquid into another liquid phase. Liquid–liquid extraction is a basic technique in chemical laboratories‚ where it is performed
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Experiment 5 Title : Thin Layer Chromatography Objectives: i. To distinguish polar and non-polar solvents. ii. To familiar with the analysis technique by using the thin layer chromatography. iii. To differentiate the retention factor‚ Rf for different compounds. [pic] Result: |Compound |Distance traveled by the compound | |o-nitroanaline |2.45
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Experiment B2 Chromatography for Protein Purification Name Matric No. Group : : : Date of Expt. : GRADE : A. Learning objectives 1. 2. 3. 4. Establish chromatographic assay to determine protein concentrations in a mixture. Appreciate the importance of resolution in protein chromatography. Understand the tension between purity and yield in protein chromatography. Understand the importance of mass balance closure in protein purification. B. Introduction I. Fast Protein Liquid Chromatography
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CHM 510 LABORATORY REPORT Experiment 1: Gas Chromatography (GC): Optimization of Flow Rate and Column Temperature Name: AFIQ B. ANWAR Student No.: 2012621072 (AS2253A) Date of experiment: Date of report submission: Lecturer’s Name: PN. HALIZA Gas Chromatography (GC): Optimization of Flow Rate and Column Temperature INTRODUCTION The main purpose of the experiment is to investigate the effects of column temperature and flow rate on the separation of methyl esters compounds
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Gas chromatography (GC) is a chromatography technique where the separation of individual components (analytes) from a sample relies on their differing distribution between a mobile and stationary phase. The mobile phase carries the analytes through the stationary phase. In GC‚ it’s an inert gas (usually helium or nitrogen). The gas must be inert‚ so it won’t react with the sample to give a false reading. The stationary phase is a substance fixed in place to which the sample adsorbs because
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Name:Shin Park|Date:06/14/2013| Exp 5: Liquids and Solids|Lab Section: | Data Table: Boiling Point Trial 1 Trial 2 Trial 3 Temperature when the last bubble has emerged ___83_˚C __82__˚C __82__˚C Average temperature from all three trials ___82.33_˚C % error ___.0849_% Melting Point Trial 1 Trial 2 Trial 3 Temperature when the acetamide melts __82__˚C _81___˚C __82__˚C Average temperature from all three trials ___81.66_˚C % error
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Ink Chromatography Lab Background Information Paper chromatography is an analytical method that is used for separating and identifying mixtures of substances into their smaller parts. Paper chromatography works because the ink used contains several dyes (pigments) that when place on porous paper are dissolved in a solvent by capillary action. When the pigments (solutes) are dissolved in the solvent (water & alcohol mixture) they move through the paper at different rates depending on their
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Component seeks to separate‚ identify‚ quantify and analyze the active component found in Biogesic®‚ which may be useful for public awareness despite its wide acceptance and availability in the market. Furthermore‚ this study applies thin layer chromatography (TLC) technique since it is the simplest‚ cheapest yet most effective approach in terms to the study’s objectives. The TLC method implies Rf (Rate of flow) values where it is given by the distance from origin to center of subtance spot over the
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Liquid Nitrogen Liquid nitrogen is nitrogen in a liquid state at a very low temperature. Its chemical formula is N2. Manufacturing of Liquid Nitrogen Liquid nitrogen is made by cooling and compressing air straight from the atmosphere. The type used to produce large amounts of liquid nitrogen looks vastly different but really happens is that air squashed and cooled . When you squash it‚ air molecules are forced closer together. If you also cool it down the molecules can slow down enough to
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Date of Experiment October 7‚ 2012 Report Submitted: October 7‚ 2012 Title: Liquids and Solids Purpose: The purpose of this experiment is to determine the boiling point of liquids and the melting point of solids. Procedure: I got all of my materials together and set up. I poured rubbing alcohol in a beaker and rubber banded it with the thermometer. I logged the last bubble that came out of the capillary tubes. After that I crushed the acetamide and carefully put it in the capillary tube
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