The mobile phase can be liquid‚ gas‚ or supercritical fluid. How Does Chromatography Work? Chromatography is a surface effect. It works by first starting off with an unidentified liquid being injected into a column. As the chemicals in the liquid move down the column‚ they separate depending on their physical and chemical attributes‚ such as speed and weight. To easily picture the process‚ the example will pertain to a paper chromatography process in which the liquid is moving over the paper‚
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Column and Thin-Layer Chromatography of Capsicum Frutescens L. Pigments John Cyril Abanto*‚ Vernalyn Abarintos and Clarice Gail Abella Department of Chemistry‚ College of Science University of Santo Tomas‚ Espana Street‚ Manila 1050 Date Submitted: September‚ 2010 ____________________________________________________________ _________________________________ Abstract: The experiment was done to separate and analyze the components of chili pepper. Chromatography was used because of its
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Diffusion is the movement of molecules from areas of high concentration to areas of low concentration. With diffusion no energy is required and it is caused by kinetic energy. The rate of diffusion can be affected by many things including temperature‚ molecular weight‚ and movement. To test the rate of diffusion a drop of dark food coloring was added to a glass of tap water and it was timed to see how long it took for the drop to reach the bottom of the glass. This procedure was repeated three
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References: 1) E. Stahl‚ Thin Layer Chromatography‚ Springer-Verlag‚ New York‚ 1969 2) Camag Scientific‚ Inc.‚ 515 Cornelius Harnett Drive‚ Wilmington‚ NC 28401‚ phone 800-3343909‚ fax 919-343-1834 3) Desaga GmbH‚ P.O. Box 101969‚ D-69009 Heidelberg‚ Germany‚ phone 0-62-21-83590‚ fax 0-62-21-840887
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It was concluded that the product created was paracetamol when a thin layer chromatography test was performed and observed under ultraviolet light (which is discussed further down the page). An unknown substance was treated with acetic anhydrate and resulted with paracetamol. A compound that behaves in this matter is 4-aminophenol and is widespread in the industrial production of this drug. Upon these observations was the build up of “Chemical A” decided. When 4-aminophenol is t treated with
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Analysis of a Volatile Liquid Abstract: The purpose of this experiment was to use various methods of analysis to determine the identity of an unknown volatile liquid. In the first part of the experiment‚ the molecular mass was found by using water to find the volume of a flask through calculations and this as well as the mass of the gas of the unknown liquid were put into the a manipulated version of the ideal gas equation to determine the molar mass of the liquid‚ which was 14.21g per
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Ion Exchange Chromatography Week 10 TAG Question 1: Chapter 6-4 of your technique book provides a detailed description of how to run a ion- exchange column. Assume you have a cation-exchange column already prepared and ready to use. Create an outline of no more than 10 steps describing how you will regenerate the column‚ load your sample and collect the hydronium ion released. 1. Open the stopcock at the bottom of the column and allow solution to drain out until the solution level in the
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Graded Assignment Honors Project 1: Liquid Breathing Write the final draft of your project. Be sure to follow these requirements and recommendations when completing your draft: • Open a new Microsoft Word document. Type your name‚ your teacher’s name‚ your school name‚ and the date at the top of your document. To help your teacher know from whom the project came‚ save the file as: CHEM_304B_10_01_Honors_Project_1_Final_Draft_FirstInitial_LastName.doc
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Volatile Liquid Purpose: To determine the molar mass of an unknown liquid whose boiling point is between room temperature and the boiling point for water. Key Terms: List and define any additional new terms relevant to this experiment. volatile – intermolecular forces - Key Formulas: Write the following formulas. Ideal Gas Law Molar Mass Relationships: The Dumas Method - In 1826 Jean-Baptist Dumas developed a method for determining the molar mass of liquids that
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EPMA Liquid Resistance Starters for Slip-ring motors. 200 to 20 000 kW. The EPMA starter has been specifically designed by AOIP for controlled starting and speed control of large slip ring motors in arduous applications such as: Crushing Milling Conveyors Pumps Ventilation In a variety of industries such as mines‚ quarries‚ cement plants‚ water treatment and associated Industries. CSE-Uniserve Pty Limited has adapted this basic design under license to AOIP to suit the harsh conditions
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