________________________12345_______________________ Date ____23.8.2013___________ Block _______ Training Lab: Paper Chromatography I. Background Information: When working in a lab‚ scientists often need to identify different molecules that are present in a sample they are studying. There are many ways to identify unknown molecules/chemicals in a sample. The method you will be using today is called Paper Chromatography and consists of 2 steps. First‚ you will separate the unknown chemicals and then you will identify
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Unversity Visca‚ Babay City‚ Leyte Name: Millama‚ Engene O. Course & Year: BS Biotech-2 Lab Schedule: W (1-4) Group 5 Date Performed: Dec. 12‚ 2012 Date Submitted: Jan. 9‚ 2013 Separation of Photosynthetic Pigment by Paper Chromatography I. INTRODUCTION There are different types of components in plant pigments. The most important and abundant chemical pigment found in plants is chlorophyll. This pigment exists in two forms; chlorophyll a and chlorophyll b. Chlorophyll a‚ being
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Gas chromatography is the common method used for the biogasoline analysis. Biogasoline catalytic cracking process requires detailed qualitative and quantitative analysis of the hydrocarbon composition of its oil and fraction. In this analysis‚ their fractional composition‚ saturated vapor pressure‚ density and antiknock rating will be investigated. By knowing the qualitative and quantitative composition of the gasolines‚ the content of n-paraffin‚ isoparaffin‚ aromatic‚ naphthene‚ and olefin (PIANO)
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Separating Water Soluble and Acetone Soluble Inks through Chromatography Abstract: Chromatography is a term used to define a set of laboratory techniques for the separation of mixtures. Through Chromatography we analyzed complex mixtures. In our recent lab we analyzed complex mixtures. The purpose of this lab was to find whether the water soluble or acetone soluble liquid traveled farther. In our recent lab we measured the distance traveled by the different colored water and acetone soluble
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understand that because substances have different properties‚ such as mass‚ that can be separated by chromatography. In our experiment‚ we chose 4 different color source to separate in water. We made sure that all the sources were water soluble because only polar substances will dissolve in water. We chose a black wet-erase marker‚ a red marker‚ a green marker‚ and mixed food coloring to test. The chromatography paper was split into 4 sections‚ about 2 cm above the bottom edge and evenly spaced out. Each
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and Combustible Liquids? Put simply‚ flammable and combustible liquids are liquids that can burn. The real difference lies within their flash point range‚ or lowest temperature at which vapours of the material will ignite when supplied with an ignition source. Flammable and combustible liquids are utilised in most workplaces‚ from manufacturing to retail and service entities. To ensure the safety of property‚ people‚ and the environment‚ both flammable and combustible liquids need to be handled
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CHEM 2204 Chromatography Lab by wyk.wong » Fri Jul 11‚ 2014 10:25 am Results and Calculations Rf values Rf=(Distance moved by the spot (cm))/(Distance moved by the solvent front (cm)) Toluene: Rf=2 cm/3.8 cm=0.53 (Fluorenone) Rf=1.1 cm/3.8 cm=0.29 (Fluorene) Hexane: Rf=1.8 cm/2.2 cm=0.82 (Fluorene) Rf=0 cm/2.2 cm=0 (Fluorene Table 1: Experimental IR peaks compared to literature IR peaks for fluorenone Functional group Experimental peak (cm-1) Literature peak (cm-1) C-H 3010.5 3013
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Title: Thin-Layer Chromatography Objectives: Part1: 1. To learn the technique of TLC and the visualization of colourless components. 2. To identify an unknown drug by a TLC comparison with standard compounds. Part 2: To learn the separation technique by using Thin Layer Chromatography plate in separating a mixture of compounds into individual pure compound by using Spinach Leaf. Introduction: (i) General Concepts Chromatography is a common and powerful method used to separate and analyze complex
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Combustible Liquids (Examples: alcohols‚ esters‚ ethers‚ ketones) Vapors from flammable and combustible liquids can mix with air and burn if they contact an ignition source. Possible ignition sources include hot electrical wires‚ hot surfaces‚ open flames‚ hot particles and embers‚ and sparks. The lowest temperature at which a liquid releases enough vapor to start burning is called the flash point. The flash point is what distinguishes a flammable liquid from a combustible liquid. Flammables:
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Alkenes from Alcohols: Analysis of a Mixture by Gas Chromatography Aim: To analyze a mixture of alkenes by gas chromatography. Introduction: In this lab‚ we specifically used elimination reaction; however we only used the E1 reaction. In the presence of strong acids‚ alcohols protonate to form a good leaving group‚ namely water. Upon loss of a proton to a good leaving group‚ an introduction of unsaturation (a double bond) can be preformed. According to Wikipedia‚ an E2 reaction is typically
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