Bangabandhu Sheikh Mujibur Rahman Science and Technology University‚ Gopalganj. Title : Assignment on HPLC (High-performance /High-Pressure Liquid Chromatography) Introduction: HPLC is an advanced form of liquid chromatography used in separating the complex mixture of molecules encountered in chemical and biological systems‚ in order to recognize better the role of individual molecules by using significantly higher pressure (50–350 bar). Compounds are separated by injecting a sample
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tomato paste. This is done using liquid/solid extraction and chromatography. Once the Lycopene is isolated‚ IR spectroscopy will determine its percentage actually obtained by chromatography. Procedure: A massed sample of 1.012g of tomato paste was placed in a 125mL Erlenmeyer flask. To the flask‚ 5mL of 50:50 hexane-acetone was added into the flask. After the 50:50 addition the flask was capped and shaken. After the settling of the sediments‚ the liquid portion was pipetted in a 50mL Erlenmeyer
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of Contents Title Page No. 1.0 Introduction 1 2.0 Sample Treatment 3 3.0 Extraction 4 4.1 Solid-Phase Extraction 4.0 Separation 6 5.2 High Performance Liquid Chromatography (HPLC) 5.3 Thin Layer Chromatography (TLC) 5.0 Detection and Identification 7 6.4 UV-Vis Spectrophotometry 6.5 Mass Spectrometry 6.6 Nuclear Magnetic Resonance Spectrometry 6.0 Health Benefits of Daidzein 18
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solids or liquids. Some mixtures can be separated into their components by simple physical methods. One method is called distillation. A liquid is boiled to produce a vapor that is condensed again to a liquid. Using this method lets say for instance you have kool aid already mixed. If you choose to boil it the water will evaporate leaving the tiny particles or sugar left behind where you can see how and what is in it to be mixed. Decanting is the simplest possible way of separating a liquid‚ pure
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consisting of two or more windings which are linked with a common magnetic field used for the distribution of electrical power from one voltage level to another. Some of the main parts of the transformer are; the windings‚ tank and insulation (solid or liquid). Transformers normally have an operating temperature range of 60-90oC and an operation lifetime of 40 years [8]. These service temperatures cause gradual degradation of the oil and paper insulation thereby producing degradation by-products such
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Limited‚ Bangalore College supervisor: Dr: T.Yasodha‚ Head- Department of Biotechnology‚ Shri Andal Alagar College of Engineering‚ Mamandur‚ Tamilnadu‚ India Objective: Purifying microbe derived therapeutics from fermentation broth by various chromatography and analysis using RP HPLC techniques- specializations on lipstatin drug. Abstract: The main focus area of this project is to purify the microbe derived drug lipstatin by using various trials of chromatographic columns with different packing
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Bhindia Pooja Rayel Title : Paper Chromatography Photochemical Smog Lecturer’s Name : Ms. Usha Nanthini Table of Content No. | Content | page | 1. | Paper Chromatography | | | 1.1 Definition | | | 1.2 Background | | | 1.3 Rf Values | | | 1.4 Principles of Paper Chromatography | | | 1.5 Procedure of Paper Chromatography | | | 1.6 Uses of Paper Chromatography | | | 1.7 Application of Paper Chromatography | | 2. | Photochemical Smog | | | 2.1
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2013 Group No.: 8 Date Submitted: January 6‚ 2014 Experiment 4: Extraction and Chromatic Separation of Plant Pigments from Tomato Paste ABSTRACT In column chromatography a mixture is dissolved in a solvent and poured over a column of solid adsorbent then eluted with the same or a different solvent. This method is often used for preparative purposes; when a relatively large amount of the mixture and the components
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and fatty acids. • Spectroscopic Techniques: Structural determination of an Organic compound by using UV & Visible ‚ IR ‚ NMR ‚ MASS techniques. • Chromatographic Techniques: Paper‚ Column ‚ Gas-Liquid chromatography ‚ High performance liquid chromatography etc. Computational Skills: Operating Systems : Win XP‚ 2000‚ 98‚ Languages : C‚ C++‚ Packages : Ms-Office. Soft Skills: 1. Good communication
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solutions: Lab report: outline Ink chromatography Chromatography is the separation of a mixture by passing it in solution or suspension or as a vapor (as in gas chromatography) through a medium in which the components move at different rates. It is used to separate substances. Examples of chromatography are finger-printing‚ medicines‚ and separating/ testing histamines. http://www.biotecharticles.com/Agriculture-Article/Applications-of-Chromatography-1299.html The purpose of this lab
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