Column and Thin Layer Chromatography Beverly Abstract: Plant pigments were separated and concentrated from a crude spinach extract through the use of column chromatography and an eluatropic series of hexanes‚ hexane/acetone‚ and methanol. The pigments were analyzed using thin layer chromatography with a 30% ethyl acetate/hexane developing solvent. Introduction: Chromatography is a technique used to separate a mixture of two or more components based on
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Gas Chromatography: Purifying alcohol Introduction The goal of this lab is to understand the principles of chromatography by purifying alcohol using fractional distillation. Running standards with gas chromatography we were able to see and calibrate our data to find not only how much ethanol our alcohol attained but also what a mixed unknown sample contained. Chromatography is a way of being able to separate substances in solution that can help not only identify the analytes (the studied
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CHROMATOGRAPHY OF PLANT PIGMENTS Marquez‚ Ma. Rica Paulene‚ Moises‚ Patrisha Kate‚ Policarpio‚ Jairus Paolo‚ Rolda‚ Zylene Joy Department of Biology‚ College of Science‚ University of the Philippines Baguio April 23‚ 2013 ABSTRACT The objective of this experiment was to apply the technique of paper chromatography as a method for separating individual plant pigments contained in plant tissue extracts containing pigment blends. The process of chromatography separates molecules because of the
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Experiment 1: Viscosity of Liquids Victoria Kulczak Lab Partners: Laina Maines & Heidi Osterman Date of Lab: 2/21/11 Due Date: 2/28/11 Abstract: The goal of this experiment was to determine the viscosity of given liquids. Two different methods were employed‚ the first measures time of flow of several methanol-water solutions‚ from point A to point B. The second method involves dropping a foreign object‚ in this case a sphere‚ into a cylinder of glycerol and measuring the time it takes for it to
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Name Date Lab: Denisty – Solids and Liquids Part 1: Density Background Information: The density of an object is how much “stuff”‚ or molecules‚ a substance is made of. Density is how tightly packed the molecules are. To calculate the density of a substance we need to know the mass (weight) of the substance and the volume (how much space) of the substance. Mass is determined by weighing an object. Volume of regular shaped objects is calculated by measuring
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Solubility as a Property of Matter A Lab of Chemicals‚ Chromatography‚ and Crime! Chemistry is a natural science that deals with the composition of matter and the changes it undergoes. At crime scenes‚ investigators often find unknown materials that need to be identified. If an unknown material is a mixture‚ an investigator may want to know one or two things about it: What are the ingredients of the mixture? Is the mixture found at the scene the same as a known mixture? A mixture is a collection
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any one factor that affects retention on paper chromatography Design and carry out a scientific investigation on any one factor that affects retention on paper chromatography CHEMISTRY LAB REPORT CHEMISTRY LAB REPORT Name: Sri Danielle Class: 9A Teacher: Sir Ajay Name: Sri Danielle Class: 9A Teacher: Sir Ajay Aim My aim for this experiment is to find out how different types of solvent will affect the retention factor in paper chromatography. Hypothesis I think that there will be a
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packed closely together‚ but they are able to move around. Hence a fluid does not have a rigid shape‚ but adapts to the contours of the container that holds it. Like a liquid a gas has no fixed shape‚ but it has little resistance to compression because there is enough empty space for the molecules to move closer. Whereas a liquid placed in a container will form a puddle at the bottom of the container‚ a gas will expand to fill the container. Although the three categories seem very well defined
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Question 1 1.1 Silica gel chromatography: This is known as the stationary phase in column chromatography. Firstly‚ the tapered exit of the column is sealed using porous material. This porous material serves as support for the packing material‚ and prevents it from exiting the pipe. Thereafter‚ silica gel is compacted into the glass pipe to make the separating column. In finishing preparation of the column‚ the solvent which is used as the mobile phase is then passed through the dry column. Then the
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The mobile phase can be liquid‚ gas‚ or supercritical fluid. How Does Chromatography Work? Chromatography is a surface effect. It works by first starting off with an unidentified liquid being injected into a column. As the chemicals in the liquid move down the column‚ they separate depending on their physical and chemical attributes‚ such as speed and weight. To easily picture the process‚ the example will pertain to a paper chromatography process in which the liquid is moving over the paper‚
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