DNA EXTRACTION FROM KIWI ISGR SCIENCE Aalah Yousif‚ Ella Sobek April 27‚ 2012 Background All living organisms (plants‚ animals‚ and bacteria) are made up of cells. Any cell consists of many parts but the parts that play the key roles are the nucleus‚ cell membrane‚ and cytoplasm. The nucleus is like the “brain” of the cell (that also contains DNA). The cell membrane is like a wall that guards the cell. And the cytoplasm is a jelly-like substance which contains other smaller organelles like
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the quantification of albumin and casein were performed and analyzed after extraction of the said proteins from their respective sources. Isolation of proteins was initiated by the breakage of the cell wall / membranes in three different ways. Homogenization of invertase‚ albumin and casein were achieved via grinding process‚ addition of 1M acetic acid and acidification by 0.1M hydrochloric acid correspondingly. Extraction of invertase and casein involved precipitation through the utilization of 95%
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(UP) INDIA November‚ 2012 Declaration I hereby declare that this project report entitled “Study of Market Performance of SERVO Lubricants in Fleet Segment within Guwahati Area” is the result of 8 weeks summer internship done by me at Indian Oil Corporation Limited‚ Guwahati under the guidance of Mr. S. Sarma‚ Dy.Manager (Technical Service)‚ NEISO‚ IOCL Guwahati. This is to further declare that this project report is authentic and not submitted by any other student previously. I also confirm
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Sautéing Fabrication: Airline Breast INGREDIENTS Airline chicken breast Salt and pepper Vegetable oil as needed 2 tbsp butter 1 finely chopped onion 2 cloves finely chopped garlic 8 oz sliced mushrooms 2 oz white wine 1 cup tomato concasse 1 cup demi-glace 2 tbsp finely chopped parsley PROCEDURE 1. Season chicken breast with salt and pepper. 2. Pour 3 tbsp oil into sauté pan over moderate heat. 3. Sauté chicken breast until browned on both sides and cook through
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Purpose This experiment deals with separating substances. The objective was to become familiar with the methods of separating substances from one another using decantation‚ extraction‚ sublimation techniques. Brief Introduction This experiment was set to teach research students different methods of separating substances from one another. Materials that are not uniform in composition are set to be impure or heterogeneous and are called mixtures. The separation of the components of mixtures is
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Processes Conversion of Heavy Residues Treatment of Refinery Gas streams INTRODUCTION: Oil refining is a key activity in the CPI. Over 600 refineries worldwide have a total annual capacity of more than 3500 x 106 tonnes. Goal of oil refining is twofold: i. production of fuels for transportation‚ power generation and heating; and ii. production of raw materials for the CPI. Oil refineries are complex plants but are relatively mature and highly integrated.
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the Indian Edible Oil Industry India is world’s third largest edible oil economy‚ after China and US. India’s annual consumption is around 10 million tones vis-à-vis China’s 14.5 million tones. However‚ India’s per capita consumption at 10.2 kgs per annum is considerably lower compared to global standards. India is also a leading producer of oilseeds‚ contributing 7-8% of world oilseed production. India is estimated to account for around 6% of the world’s production of edible oils. Though it has the
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EXTRACTION OF DNA FROM ONIONS ABSTRACT The purpose of the experiment was to experience firsthand the isolation of DNA form a plant tissue without destroying its structure and sequence. A white onion was used for the experiment. After several processes‚ DNA isolate was the visible result. Different chemical tests were performed on the DNA isolate‚ namely: Dische Test‚ Murexide Test‚ Wheeler-Johnson test and Test for Phosphate. Visible results were then noted. INTRODUCTION DNA (deoxyribonucleic
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The purpose of this experiment is to separate a solution of an acidic and organic compound and purify its respective acidic product through the techniques of an acid base extraction‚ recrystallization‚ and use of Melt Temp (melting point range). The reaction of an unknown acid (3-chlorobenzoic acid) with diethyl and sodium hydroxide was observed‚ and the solution naturally separated into an aqueous and organic solution. The mixture of acid and organic solution was created and then separated through
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DNA Extraction Lab Purpose: To compare the amount of DNA extracted from two different species‚ despite using the same method. Hypothesis: I predict that the liver will produce a higher quantity of DNA than the strawberry. This is because I believe that animals have a higher DNA yield because our structure is more complex than a plant’s structure. Materials: -Sample of Strawberries -Zip lock bag -DNA extraction buffer -Cold ethanol -Glass rod -Double ply cheese cloth -Two test tubes
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