SBI 4U0: Enzyme Lab Purpose: To compare the action of the enzyme catalase‚ to a non-protein catalyst under different conditions. Observations: | | |Observations |Rate of Reaction |Interpretations | |A |Sand |- Sand piled up at the bottom of |0 |- There is no reaction between sand and| | |
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September 16‚ 2011 September 26‚ 2011 Abstract Catalase is a common enzyme found in nearly all living organisms that is frequently used by cells to rapidly catalyze the decomposition of hydrogen peroxide into less reactive oxygen and water molecules. Catalase is a protein that is most commonly found in the liver. The purpose of this experiment was to determine the effect that changes in temperature and pH have on the function of the enzyme catalase. Five test tubes were collected and cleaned. The
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the enzyme‚ catalase. Living tissues produce the enzyme catalase‚ which is able to break down hydrogen peroxide into oxygen gas and water. The reaction is shown below: H2O2 O2 + H2O Cells make hydrogen peroxide as a normal product of metabolism. But it is toxic to the cell if it builds up. Therefore‚ all cells produce catalase to get ride of hydrogen peroxide. In the following experiment‚ we will use liver as a source of catalase. Before
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Materials: Fresh liver (will be cut into 12 small cubes with equal size). Frozen works fine | Thin spatulas to scrape liver from mortar into test tube | 4 test tubes and stand | Fine sand | 3% hydrogen peroxide | 100mL breaker | Mortar and pestle | 10 mL Measuring cylinder | Bunsen burner/hot plate | Detergent | 1M Hydrochloric acid (HCL) | Ruler | Matches | Knife | Procedure: 1. Cut 12 small cubes of liver (under 1 cm)‚ of equal size. 2. Put 3 liver cubes into a test tube
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When liver was added to hydrogen peroxide is caused and instantaneous reaction time. This is due to the enzyme catalase. If the enzyme catalase was not present in the human body‚ hydrogen peroxide‚ which is highly toxic to cells‚ would attack the unsaturated fatty acid compounds in the membrane of lipids.2 This would cause substantial damage to the membrane structure. Since catalase utilizes hydrogen peroxide as both an electron acceptor and donor it oxidizes hydrogen peroxide isolating oxygen
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• Raw liver • Forceps • Petri dish • Labeling tape and pen • Dropper pipette • Ice bath • 0.1% hydrogen peroxide solution • 3 thermometers • Liver puree • Warm-water bath • 25-mL graduated cylinder • Clock or watch with second hand • 5 50-mL beakers • Paper towels • Filter-paper disks Part A: Observe the Catalase Reaction 1. Put on apron and safety goggles. Use forceps to place a small piece of raw liver in an open petri dish. Use a dropper pipette to put a drop of hydrogen peroxide
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there a relationship between catalase concentration and the rate of reaction? Hypotheses: if the enzyme concentration is increased then the rate of reaction will increase. If the enzyme concentration is decreased then the rate of reaction will decrease. Controlled: temperature Independent: % catalyze concentration ( Dependent: rate of reaction (mm/s) Materials: Mortar and pestle Graduated cylinder Distilled water Test tubes Chicken liver (or any other liver) Sterile transfer pipet
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Investigation Aim: The aim of the investigation was to investigate the effect of substrate concentration Hydrogen Peroxide H O (in %) on the rate of reaction of the enzyme catalase (in 1/mean time). Prediction: As the substrate concentration (hydrogen peroxide) in % increases the rate of reaction in 1/mean rate increases until the solution becomes saturated with the substrate hydrogen peroxide. When this saturation point is reached‚ then adding extra substrate will make no difference. The rate steadily
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creative name)‚ the enzyme catalase was observed under varying conditions in order to interpret what prohibits and inhibits the functioning of certain enzymes in chemical reactions. Enzymes aid in chemical reactions by speeding up the time it takes for the reaction to occur‚ without getting “used up” throughout the process. Catalase‚ the specific enzyme used in this lab‚ is a protein abundant in the liver and red blood cells. It enhances the breakdown of hydrogen peroxide‚ the substrate‚ into oxygen
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and flaws within human reflexes. · Size of the liver cubes: As the liver cubes needed to be cut by each individual group‚ the sizes between the groups may have varied. The required size for the lamb liver cubes was 1cm3 ‚ however the task of cutting the liver into all the same was extremely difficult‚ keeping the size consistent throughout the nine other groups would be close to impossible. It was important to have a consistent size in the liver pieces‚ this may have been achieved by a number of
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