AP Biology Lab: Catalase (Enzymes) Abstract In this laboratory exercise‚ studies of enzyme catalase‚ which accelerates the breakdown of hydrogen peroxide into water and oxygen. The purpose was to isolate catalase from starch and measure the rate of activity under different conditions. The laboratory was also conducted in association with a second laboratory that measured the effects of an inhibitor on the enzymes. Changes in temperature and pH along with Substrate Concentration and Enzyme
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affect the way catalase‚ the enzyme tested‚ denatures‚ or breaks down. Hypothesis: If the potato is acidic‚ it will react with the H2O2 more than it will with the raw‚ plain potato because the acid will denature the enzyme faster. The manipulated/independent variable is the raw‚ plain potato while the responding/dependent variable is the other types of potatoes used in the experiment. Materials: Test tube rack 4 test tubes Graduated cylinder Beaker 10-15 mL of hydrogen peroxide Potatoes
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Unknown Lab Report Microbiology Unknown A Sonia Kabra November 26‚ 2014 Introduction There are numerous reasons for identifying unknown bacteria. Some of these organisms have distinct qualities that set them apart from one another‚ such as the exposure to certain environments. Through out the semester in the laboratory‚ we are able to encounter some of the few microorganisms that we as humans have come into contact with. With the knowledge gained from the sessions in the laboratory‚ we can now
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Enzyme Kinetics and Protein Determination: How Enzyme Catalase Concentration Affects Reaction Rate and Determining the Identity of Unknown Proteins through Absorbance By: Alexander Mak 7238991 Partner: Yasmin Ismail BIO2137 Section A7 Corrector: Chieu Anh Ta September 18‚ 2014 Introduction: The first lab’s primary objective is to observe the different reactions rates amongst the five different catalse concentrations of parsnip. The rate at which the enzyme catalyzes increases in
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before measuring out the catalase and hydrogen peroxide. After assembling the apparatus‚ we filled a syringe with 1 mL of catalase and placed it on ice. We then filled an Erlenmeyer flask with 10 mL of hydrogen peroxide and sat that in a 45°C water bath. We allowed the flask to sit in the water for three minutes before adding the catalase‚ so that the hydrogen peroxide could equilibrate. After three minutes we attached the stopper assembly to the flask and dispensed the catalase‚ making sure to close
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Part 1A Analysis questions: 1. How many “chainobeads” was your enzyme able to make per minute in the 0 – 15 second interval? Our enzyme was able to make 6 chainobeads in the 0-15 interval. 2. How many “chainobeads” was your enzyme able to make per minute in the 60 – 120 second interval? Our enzyme was able to make 49 chainobeads in the 60-120 intervals. 3. Did your enzyme’s rate change over time? How does this compare to a real enzyme? The enzyme’s rate did change over time. This compares to a
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the possibilities of collisions decrease. Less reactions happen at lower temperatures‚ which results in the hydrogen peroxide taking longer to break down. When doing our own experiment we saw this exact thing happen‚ and you can see this in our results. There is a clear difference between temperatures lower than 25ºC and higher than 55ºC‚ in the way the catalase broke down the hydrogen peroxide. From our results‚ it’s clear that these temperatures were enough to break the bonds in enzyme and then change
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Glucose Oxidase and Its Various Uses Aaron Truong Since glucose oxidase has an end product called hydrogen peroxide‚ which is a harmful substance to bacteria‚ it can be used to fight bacteria‚ or sterilize objects (can have various uses such as in hand sanitizers‚ toothpaste‚ soap‚ etc)‚ not just biosensors. Another key part in the reaction would be C6H12O6‚ or glucose. Glucose oxidase can be applied to diabetics as mentioned earlier‚ as biosensors work by "keeping track of the
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which catalase decomposes H2O2. After adding H2SO4 for different time lashes‚ etc.‚ the resulting data will be graphed at which the catalase decomposed by catalase. Background: The four different activities to the enzyme catalyst lab have similar but different backgrounds. Activity A’s background is to investigate the specific reaction of the decomposition of hydrogen peroxide by the enzyme‚ catalase. Hydrogen Peroxide decomposes slowly into water and oxygen‚ and the addition of catalase lowers
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enzymes helps us to identify bacteria -used to evaluate metabolic capabilities of specific bacteria. 1. Catalase Test- Enzyme: Catalase Substrate: Hydrogen Peroxide Reagent: Hydrogen Peroxide Product: Oxygen Medium: TSA Bubbles (+)- Catalase… No Bubbles (-)- No Catalase 2. Denitrification Test- Enzyme: Nitrate Reductase Substrate: Nitrate Reagent:
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