IDENTIFYING GRAM POSITIVE COCCI As mentioned in Exercise 8‚ “Identifying Gram Negative Rods”‚ identifying bacteria is a common activity in the microbiology lab. Like the game Clue™‚ each time you gather a piece of information to solve the mystery‚ you gather some information that supports some identities and eliminates others from contention. In the lab‚ the process continues as you gather more information until only one microbe remains and all others have been eliminated as possibilities. Thus
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Investigation on the effects of Temperature‚ pH levels‚ and Enzyme Concentration on the reaction rate of the Decomposition of Hydrogen Peroxide by Catalase The purpose of this investigation is to figure out how temperature‚ pH‚ and enzyme concentration affect the reaction rate of an enzyme. It’s important to understand how certain factors affect enzymes because of their crucial role in the metabolic processes of life. Enzymes lower the activation energy necessary for a chemical reaction to
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IB Biology Sheran Gu Meghan Lena Nov. 15th Influence of pH on Enzyme Activities How does the pH of solution influence the speed of enzyme activities? Enzymes are protein catalysts and chemical that speed up chemical reaction without being involved in the reaction. They are involved in the human digestive system to help people digest food more efficient. Food are substrates to be break down and absorb through the digestive system in the body. Substrate is a molecule that bond with the active site
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How is pH affecting catalase activity? Hypothesis The catalase activity is assumed to be the most active in pH7. The higher or lower concentration away from the optimum pH of catalase‚ the slower the rate of activity is. Data Collection & Processing I collected the results of pH4‚ pH7‚ pH10 and pH13 after 2 minutes‚ and I repeated the experiment 3 times. Below is a table to show the results‚ their averages and standard deviations: | 1st time (sec) | 2nd time (sec) | 3rd time (sec)
Free PH Buffer solution Standard deviation
in cells. Enzymes can be found in both liver and potato. They provide a site where reactants can be brought together to react. These reactants are called substrates that fin into the enzyme. The changing of heat‚ surface area‚ chemicals‚ and pH will denature the shape of the enzyme‚ not allowing it to do its function. Testing on peroxidase- an enzyme- will be done on an animal enzyme (chicken liver) and a plant enzyme (potato). For testing Hydrogen Peroxide (H2O2) will be poured over the enzymes
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because all temperatures except 28⁰C was collected once from peers. Based on the results from the graphs‚ at extreme temperatures such as 0⁰C which is extremely cold and 100⁰C which is tremendously hot; catalase activity performed better at 0⁰C than at 100⁰C. Strangely‚ 50⁰C outperformed 37⁰C. Catalase activity was higher for intermediate temperatures such as 37⁰C and 50⁰C than the extreme temperatures such as 0⁰C and 100⁰C. Overall the rate of reaction peaked at 28⁰C and every other temperature had
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into the wonderful world of catalase. Beginning with establishing a base line of just how much hydrogen peroxide there is in 5.0mL of the reacted solution; to figuring out exactly how much actually reacted after 300 seconds of catalyzed reaction. Follow the experiment from the beginning steps right to the end as you see where the students went wrong‚ interpretation of the results‚ and great answers to work sheet questions. Now‚ dive into the amazing world of catalase catalysis. Background Information:
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Enzyme kinetics (catalase/kmno4) Enzyme catalysis Farah Mohamed galal 22-3014 t09 Introduction: E + S → ES → E + P Enzymes are proteins which act as a catalyst in biochemical reactions(affect the rate of achemical reaction). The substrate binds to the active site of the enzyme. Any deformation of the active site will affect the activity of the enzyme‚ these are some ways that enzyme action may be affected because of them: 1- Salt concentration: If it is close to zero or very high
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OBJECTIVE: 1. To distinguish the bacteria abilities to metabolize various substrates and end products formed. 2. To observe the growth of different bacteria species in term of structures and its morphology based on different chemical substance applied. 3. To observe physiological and immunological properties utilized by different species of bacteria. INTRODUCTION: Bacteria biochemical testing can determine the types and numbers in terms of colony forming units of bacteria present in a
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f) Product of the reaction In my investigation‚ I will be using the enzyme catalase‚ which is found in most living organisms. It catalyses the decomposition of hydrogen peroxide into water and oxygen. 2H2O2 + Catalase > 2H2O + O2 Catalase dramatically reduces the activation energy needed for the reaction. Without catalase the decomposition would take much longer‚ and would not be fast enough to sustain human life. Hydrogen peroxide is also a dangerous‚ very potent by-product of metabolism‚ so it is essential
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