activity using hydrogen peroxide. Hypothesis: My hypothesis for this experiment is that temperatures near body temperature is when enzyme activity will be at its highest. I believe this will occur because in our body‚ enzymes are very efficient. I also think that as the temperature of the water increases‚ there will be more enzyme activity. Materials: 1. Water/Ice 2. Hot Plate 3. Test Tube 4. Liver 5. Sand 6. Cork 7. Small Circles of Filtered Paper 8. Tub 9. Hydrogen Peroxide 10. Graduated Cylinder
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Many organisms can decompose hydrogen peroxide (H2O2) enzymatically. Enzymes are globular proteins‚ responsible for most of the chemical activities of living organisms. They act as catalysts‚ substances that speed up chemical reactions without being destroyed or altered during the process. Enzymes are extremely efficient and may be used over and over again. One enzyme may catalyze thousands of reactions every second. At the start of the reaction‚ there is no product‚ and the concentration is the
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enzymes that catalyze these reactions and how these biochemical reactions affect the liver and brain and the effects of alcohol on the human body. Introduction A good deal of the population consume alcohol‚ some more than others. The effects of alcohol consumption on the human body affects mainly the central nervous system‚ however alcohol is mainly metabolized in the liver and can weaken or damage the liver making it less efficient in metabolizing substances that the body requires to carry out
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widely used in wine production. 4. Textiles The use of enzymes in textile industry is one of the most rapidly growing fields in industrial enzymology. The enzymes used in the textile field are amylases‚ catalase‚ and lactases which are used to remove the starch‚ degrade excess hydrogen peroxide‚ bleach textiles and degrade lignin. 5. Animal feed Addition of xylanase to wheat-based broiler feed has increased the available metabolizable energy 7-10% in various studies. Enzyme addition reduces
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Activators are molecules other than the substrate that interact with the active site and they help increase the rate of reaction. Inhibitors bind and react with the side chains and slow the rate of reaction. In this experiment‚ hydrogen peroxide (H O ) acted as the substrate being broken down while 2 2 the catalase was the enzyme. The hydrogen peroxide formed a decomposition reaction as it was broken down to water (H O) and oxygen (O ). The balanced equation is evident below. 2 2
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Bibliography: Aebi‚ Hugo. 1984. Catalase in vitro. Methods in enzymology; 105 Academic Press: 121126. Aiyegoro Olayinka A and Anthony I Okoh. 2010. Preliminary phytochemical screening and In vitro antioxidant activities of the aqueous extract of Helichrysum longifolium DC. BMC Complementary
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onto TSA/ MAC allowed for the growth of gram (-) and would be used for further test. Procedure: The catalase test was performed only on gram (+) bacteria‚ as this test would not help in differentiating the gram (-) bacteria because all of the possible unknown gram (-) bacteria were catalase positive. This test is used to detect the presence of catalase‚ which helps to breakdown toxic hydrogen
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upper respiratory tract‚ including most species of Streptococcus and Staphylococcus‚ is to inhibit the growth of aerobic organisms through the presence of oxygen and high concentration of tellurite. Reactive oxygen species compounds such as hydrogen peroxide (H2O2)‚ superoxide anion (O2-) and hydroxyl redical (OH-) are natural byproducts of the normal metabolism of oxygen that can be formed by exposure of cells to free-radical generating molecules like metals and metalloids. ROS compounds are generally
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The catalase test is used to differentiate staphylococci (catalase-positive) from streptococci (catalase-negative). The enzyme‚ catalase‚ protects the bacteria from the toxic by-products of oxygen metabolism. This enzyme is produced by bacteria that respires using oxygen. The catalase-positive bacteria include strict aerobes. Catalase-negative bacteria may be anaerobes‚ or they may be facultative anaerobes do not respire using oxygen as a terminal electron acceptor. The test reaction is very fast
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substrate concentration on enzyme activity * Catalase – an enzyme that catalyses the conversion of hydrogen peroxide into water and oxygen * Source of catalase is potato: amount of oxygen produced is amount of enzyme activity * 5 test tubes‚ different amounts of H2002 and distilled water‚ measure height of bubbles of oxygen * Results: an increase in substrate concentration produces an increase of the activity of the enzyme catalase Test the effect of temp on the activity of enzymes
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