Jeffrey Shenfeld An Experimental and Statistical Analysis of SDH activity as compared in Liver‚ Kidney‚ and Heart Homogenates of the Bos taurus Methods The three tissues being analyzed in this experiment‚ those of the kidney‚ heart and liver‚ were taken from the animal Bos taurus. The tissue homogenates used were made by adding 1 gram of tissue to 20 ml of sucrose phosphate buffer. The buffer was composed of 250 mM sucrose‚ 50 mM NaPO4‚ with a pH of 7.4. This mixture was homogenized with
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ENG 111 Silent Killer The liver helps digest food‚ absorb nutrients and expel toxins from the body. Recent studies show that one in ten children in US is thought to have a liver disease that was thought to afflict alcoholic adults. The condition has become very common in non-drinkers that it has been dubbed nonalcoholic fatty liver disease. Whenever someone gets the disease‚ the organ becomes bloated and discolored due to the fatty cells. This disease is alarming doctors because it progresses
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Preliminary Experiment (4% yeast concentration) Hydrogen peroxide volume – 5 cm3 Water Volume -0 cm3 Concentration Volume- 20 vols Time in Seconds Volume of O2 (cm3) Experiment 1 Experiment 2 Average 30 95 94 94.5 60 100 100 100 90 100 100 100 120 100 100 100 150 100 100 100 180 100 100 100 Modifications The results from my preliminary experiment show that 100 cm3 of oxygen has been produced in the first 30 seconds.. This reaction is far too quick and will prevent me from analysing
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above a certain point‚ an enzyme can be denatured. Enzyme concentration‚ substrate concentration and inhibitors are other factors affecting enzyme functionality. The substrate in this lab is Hydrogen Peroxide (H2O2) and the enzyme is catalase in the liver‚ which locates in almost all living organisms and prevents the accumulation of toxic levels of hydrogen peroxide by decomposing hydrogen peroxide to water and oxygen. (2 H2O2 → 2 H2O + O2) Purpose To investigate and quantify an enzymatic reaction
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1. Liver disease can occur due to diabetes & so can diabetes occur due to liver disease. The liver disease occurring due to diabetes‚ primarily type 2 diabetes is called Non-Alcoholic Fatty Liver Disease (NAFLD). Type 2 diabetes is metabolic condition causing elevated blood glucose levels due to either the lack of production of insulin or the body’s inability to utilize insulin efficiently (PubMed‚ n.d). NAFLD or commonly called fatty liver‚ occurs due to impairment of the liver cells to effectively
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|Liver did not change in size or shape |peroxide was added) | | | |Bubbles rose and was large in size |2 | | | |(after hydrogen peroxide was added) | | |PART B |Liver in acidic acid |Liver had a change
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catalase‚ located in the liver‚ is being used to break down the substrate. Problem The purpose of this lab is to see if the enzyme‚ catalase‚ can be manipulated. We are testing the effects of pH‚ temperature and enzyme concentration. Hypothesis Controlled When we add peroxide to the liver it will bubble a lot and break down faster. When we add peroxide to the liver it will not bubble a lot and it will not break down faster. pH When acid is added to the liver‚ it will increase the reaction
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H2O2 into water and oxygen | | | |the tube | |gas with a lower activation energy | |B |Liver |- Reaction occurred right away‚ |4 |- Liver contains large amounts of the | | | |and big‚ white bubbles rose the | |enzyme catalase‚ which break down H2O2.| |
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pieces of liver. Also‚ hydrogen peroxide was used to demonstrate these effects. Storyboard: Materials: 2 50 mL beakers 10 mL graduated cylinder 3% hydrogen peroxide solution Hot water bath Lemon juice or HCl Fresh liver Forceps Procedure: Measure 10 mL of hydrogen peroxide and record its temperature. Pick the liver up with the forceps and add it to the hydrogen peroxide‚ record the temperature once more. Record the reaction. Using the same piece of liver‚ repeat
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temperature on enzyme In a liver sample were observed under iced‚ boiling‚ 37 degrees‚ and room temperature Conditions. The enzymes became completely denatured under boiling conditions because they couldn’t take the intense heat. Enzymes performed in optimal conditions of 37 degrees. But When the enzyme were iced the enzymes reacted slowly. They stop because of the iced Conditions stopped them from moving freely‚
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