As a teenager‚ Lucas’ mother was a prostitute and caused him to hate women by forcing him to be a bystander while she performed sexual acts with various men. This was ongoing occurrence for years‚ but as Lucas got older he would often go home late or not go home at all in hopes of missing this common practice of his mother’s. There would be consequences once Lucas did return home for missing his mother’s acts such as beatings. His first crime
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the ninth tube was labelled ‘Negative control. all the sample tubes 1b‚ 2b‚ 3b and positive control b‚ 0.5 ml of reagent 1 and 50 µl of reagent 2 and 0.5ml of saline was added ‚and To the sample tubes 1a‚ 2a and 3a and positive control a‚ 1.0 ml of 0.9% saline and 50 µl of reagent 2 was added to them and To the negative control tube‚ 600 µl 0.9% saline‚ 0.5 ml reagent 1 and 50 µl reagent 2 was added and all 9 tubes were placed in 37oC water bath for 15 minutes to allow the reaction to finish‚ the
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Biuret Reagent 15. Iodine 16. Benedicts Reagent Methods In this lab there are I complete two different tests. The procedure was relatively the same for each test. I would first add 3ml of a given substance to a test tube‚ followed by 5 drops of either Biuret Reagent or Iodine. Once the agent is added‚ I swirled the two substances and then observed the results. The first was to test proteins‚ and the second‚ was to test starches. In the first test‚ Proteins‚ I used biuret reagent to determine
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To determine if the reaction had gone to completion‚ a Starch/Iodine test was conducted. A change in the strip to a deep blue color would signal an excess of sodium hypochlorite reagent present. An excess is the reagent symbolizes the end of the reaction because there is no longer any reactant left to consume reagent. After the reaction was deemed completed‚ the product was isolated and purified by ether extraction‚ aqueous extraction and evaporation. To determine how sodium hypochlorite acts as
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To determine protein concentration in apple juice. Procedure Part1 7 test tubes were prepared and labeled. The appropriate amount of BSA and distilled water were pipetted into the respective test tubes according Table 3.1. 500µl of Branford Reagent were added into all the test tubes. The test tubes were all vortexed and mixed.
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The Bradford protein assay is a spectroscopic analytical procedure used to measure the concentration of protein in a solution [1]. There are several reagents that can be used to determine the concentration of protein in solution which are Bradford reagent‚ Biuret reagent‚ Lowry protein assay and many more. In this experiment‚ the Bradford Reagent is used to measure the protein concentration. Marion M. Bradford had developed the Bradford protein assay. The Bradford assay is a colorimetric assay based
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Synthesis and Structure of Alcohols Alcohols can be considered organic analogues of water. H O H R O H Alcohols are usually classified as primary‚ secondary and tertiary. H R H OH H primary R R OH R R OH OH R secondary tertiary phenol Alcohols with the hydroxyl bound directly to an aromatic (benzene) ring are called phenols. Nomenclature of Alcohols (Normally any compound’s name which ends in –ol is an alcohol of some sort) IUPAC rules that:
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Department of Chemistry‚ University of Santo Tomas‚ Manila‚ Philippines Abstract The Bradford method used to determine the protein content of a certain solution (Menguito‚ 2010) and involves the acidic Coomassie Brilliant Blue G-250 as a coloring reagent. [1] The dye is originally pinkish-brown in color when it is in its acidic state. When protein is bound to the dye the color turns blue. In this experiment a standard was used to start the experiment‚ specifically the BSA stock solution. This standard
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addition of a Grignard reagent to an aldehyde/ketone = stable tetrahedral intermediate (alkoxide) - addition of an alcohol to a carbonyl group in the presence of a base = unstable intermediate (hemiacetal/hydrate) - nucleophiles with good leaving groups (anions i.e. Cl-‚ RO-‚ RCO2-) = unstable - starting carbonyl compound with good leaving group = unstable (makes a Tetrahedral intermediate then collapses to form the starting carbonyl group) i.e. Grignard reagent added to an ester
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Lucas is trying to argue that Jesus Christ was really a founder of Socialism since his “whole being was one flame of hatred for wealth‚ and all that wealth stands for...” Lucas claims that in regards to the type of life Christ lived that he is totally against the rich people of the world. Lucas even goes and quotes scripture to back himself up; however‚ all the scripture he uses is taken largely out of context. For example‚ Lucas quotes the first section of Matthew 6:19‚ “Do not store up for yourselves
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