Department of Chemistry‚ University of Santo Tomas‚ Manila‚ Philippines Abstract The Bradford method used to determine the protein content of a certain solution (Menguito‚ 2010) and involves the acidic Coomassie Brilliant Blue G-250 as a coloring reagent. [1] The dye is originally pinkish-brown in color when it is in its acidic state. When protein is bound to the dye the color turns blue. In this experiment a standard was used to start the experiment‚ specifically the BSA stock solution. This standard
Premium Amino acid Coomassie
Lucas is trying to argue that Jesus Christ was really a founder of Socialism since his “whole being was one flame of hatred for wealth‚ and all that wealth stands for...” Lucas claims that in regards to the type of life Christ lived that he is totally against the rich people of the world. Lucas even goes and quotes scripture to back himself up; however‚ all the scripture he uses is taken largely out of context. For example‚ Lucas quotes the first section of Matthew 6:19‚ “Do not store up for yourselves
Premium Christianity Jesus Religion
acids. What atom is present in an amino acid that is not present in a sugar molecule? One nitrogen atom is present per molecule in an amino acid. Data Collection: Sample Before Heating A. Benedict’s Solution +Heat B. Iodine Solution C. Biuret Reagent 1. Protein Solution Purple Light Purple Dark Orange Light Purple 2. Glucose Solution Light Blue Orange-yellow Light Orange Transparent
Premium Glucose Carbohydrate Starch
of one of the ions or anions combined with one of the reagents such as NaCl. It was found that it could be separated out after centrifuging and would result in a sample without the ions or anions if it precipitated. With the data from week one a method of adding the reagents in a certain order would result in the ability to separate all ions or anions out of a solution. Overall‚ knowing how the cations or anions reacted with certain reagents benefited greatly to determine a way to create a separation
Premium Chemistry Solubility Separation process
addition of a Grignard reagent to an aldehyde/ketone = stable tetrahedral intermediate (alkoxide) - addition of an alcohol to a carbonyl group in the presence of a base = unstable intermediate (hemiacetal/hydrate) - nucleophiles with good leaving groups (anions i.e. Cl-‚ RO-‚ RCO2-) = unstable - starting carbonyl compound with good leaving group = unstable (makes a Tetrahedral intermediate then collapses to form the starting carbonyl group) i.e. Grignard reagent added to an ester
Premium Alcohol Hydrogen Oxygen
ferric chloride‚ Bariit’s A and B solutions‚ Kovac’s reagent‚ zinc dust‚ hydrogen peroxide‚ API 20E incubation tray‚ Bunsen burner‚ oxidase reagent and bacteria Methods: The method used for the API (analytical profile index) 20E System was the aseptic technique was used for the inoculating loop to get a unknown colony from the streak plate or pure culture slant. A small amount was smeared over the filter paper and several drops of oxidase reagent were added and the color change was noted. Another
Premium Hydrogen peroxide Bacteria Amino acid
Lesson 1 Key Questions 1. Mg2+ has 10 electrons 2. Given that the half life of the radioisotope carbon -14 is 5730 years‚ it would not be useful in dating bones that are over a million years old. After 40 000 years of age less than 1% of the 14C is left in the bone and thus it is not useful for determining the exact age beyond that. 3. Hydrolysis - A chemical reaction where a chemical is broken down by a reaction with water. Hydrolysis is important for the digestion of food‚ making
Premium Carbon Isotope Proton
epigraph to his 1910 novel Howards End: "Only connect". In part one of The Road from Colonus‚ Mr. Lucas and her daughter travelled to Greece‚ to fulfill his dream which has lasted for 40 years. When they were in a Khan in a small town of Greece‚ Mr. Lucas discovered a very strange tree which has a stream flow from it. He was very excited and felt that he had found his “habitant for heart”‚ so Mr. Lucas wanted to stay there for more time‚ but his daughter frustrated him and finally they left Greece
Premium Sociology E. M. Forster Old age
1977‚ it has been called one of the best movie series of all time by many fans. The original Star Wars (Episode IV: A New Hope) shot the top of off the box office and has been a classic since then. In a galaxy far‚ far away‚ a man named George Lucas has been directing a movie of his called American Graffiti‚ while filming the movie‚ he gets a great idea‚ a movie‚ a space western to be exact. It’s a beautiful idea and he starts working on it right after American Graffiti. He starts to write this
Premium Star Wars Episode IV: A New Hope Star Wars Star Wars Episode VI: Return of the Jedi
| Gram Negative Unknown | Biology 3444-006 | | Lena Wallace | 11/7/2011 | | Abstract: The purpose of this lab was to identify an unknown bacteria culture using differential tests. The identification of the unknown culture was accomplished by identifying the bacteria based on its specific metabolic characteristics and morphology. It is suggested that culture 11 is a sample of Enterobacter aerogenes. Introduction: This experiment was centered on metabolic and biochemical testing
Premium Staining Gram staining Enzyme