since they are readily carried about by air currents. Septa = hyphal cross walls which divide the filaments into separate cells. Petri Plate (dish) = a special covered dish in which mold is cultured. Medium = a solid nutrient used for culturing. Agar = a non-nutrient thickening agent which is thicker than gelatin but still quite soft. Smear = a thin film of microbial cells on a microscope slide. Fixing = passing the smear through the flame of the laboratory burner three times in rapid succession
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Unknown Lab Report Unknown Organism #6 Ann Le (Phuoc) May 6‚ 2010 Dr. Carrington Microbiology Lab- MW 12:50 Le 1 I. Introduction My unknown organism #6 is Morganella morganii‚ which is a gram-negative bacillus rods commonly found in the environment and also in the intestinal tracts of humans‚ mammals‚ and reptiles as a normal flora. (3‚ 5) This bacterium Morganella morganii‚ was first discovered in the 1906 by a British bacteriologist named H. de R. Morgan. (2) Despite its wide
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used to test antimicrobial activity against disease causing bacteria Staphylococcuaureus. Extracts of varying concentrations of Azadirachtaindica fruit pulp and leaf extract were prepared using Phosphate Buffer and tested against test organisms using agar diffusion method. Oxfloxacinof same varying concentrations were used to compare the effect of antimicrobial activity of fruit pulp and leaf extract. Keywords: Azadirachtaindica‚ Antimicrobial activity‚ Staphylococcus aureus. I. INTRODUCTION Azadirachtaindica
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a given sample. It enumerates the number of actual live‚ viable cells in the sample that form colonies on a suitable agar medium. As the optimum medium and conditions varies for one sample to another‚ the colony count methods provide an estimate of the number of viable cells according to the medium employed‚ time and temperature of incubation. Each colony that appears on the agar plate arising either from a clump of cells or from a single cell is referred as a colony forming unit (CFU). The sample
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CHAPTER ONE 1.0 INTRODUCTION Natural rubber is produced by over 2000 plants species and its main constituent is poly (cis-I‚4-isoprene).a highly unsaturated hydrocarbon. Since 1914 there have been efforts to investigate microbial rubber degradation: However‚ only recently have the first proteins involved in this process have been identified and characterized and have the corresponding genes cloned. Analysis of the degradation product of natural rubber and synthetic rubbers isolated from various
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nutrient agar plates and using a permanent marker draw four quadrants on the bottom of each agar plate. Using a sterile pipet transfer 250 ml of E. coli broth to the middle of each petri dish and evenly spread bacterial culture around the agar plate. Cover and allow the culture to soak into agar for at east 15 minutes. Using sterile forceps‚ carefully place one filter disk from designated sample into the middle of each
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controlled variable is important in order to be able to look at what the bacteria would look like if it hadn’t been contaminated and just left as agar. Having a sample of agar that wasnt exposed to any bacteria will provide a clear picutre of what grew on the agar upon feeding bacteria to it. 2. Why shoudn’t a student swab his or her mouth or cough onto an agar plate to initiate a culture? Even though most bacteria in the human body is harmless‚ if one was knowingly or unknowingly ill then harmful
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plate and the streak plate inoculation procedure for the separation of the cells of a mixed culture so that discrete colonies can be isolated. ii. To prepare a stock culture of an organism using isolates from the mixed cultures prepared on the agar streak-plate and/or the spread plate. Introduction : In order to be able to adequately study and characterize a certain microorganism‚ microbiologists need to separate and isolate this microorganism from the many other microorganisms with which
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_____________________________________________________________________________ Agarose is a polymeric cross-linked polysaccharide extracted from the seaweed agar. Agarose is used widely in gel electrophoresis because it gels at a lower temperature‚ does not contain the inhibitors of virus growth frequently present in agar‚ and has more uniform pore size than that of agar. It is also easily poured and does not denature the samples. In agarose gel electrophoresis‚ DNA or RNA fragments are separated or isolated according
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A2 Practical Investigations Title: The effect of Temperature on the growth of Aspergillus oryzae Develop a Hypothesis This particular investigation is to discover how a range of temperatures effects the growth rate of the fungi Aspergillus oryzae. Most fungi’s tend to survive within the temperature range of 5-35oC‚ with the optimum depending on their normal environmental temperature. The fungi Aspergillus oryzae is heterotrophic which means they taken in their food from dead organic matter and cannot
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