ASEPTIC TECHNIQUES AND SOURCES OF MICROBIAL CONTAMINATION. Introduction The spread of infections has come to a point where it has become catastrophic. Aseptic technique is the method used to prevent contamination of infections. It is widely used in hospitals‚ pharmacy‚ and pharmaceutical industries and in laboratories. Different establishments have come up with more ways to improve infection control. In hospitals health care acquired infections are costing the NHS £1 Billion a year and
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Shakira Jarvis Microbiology Lab Assignment Laboratory Assignment Outline 1. Check in & The Microscope a. Review of proper lab etiquette. i. Review laboratory syllabus and b. Review of the Parts of a Microscope ii. Review of lab exercises about different types of Microscopes 2. Survey of Microorganisms c. Viewing‚ drawing‚ and describing several types of fungi‚ algae‚ and Bacteria iii. Chlamydomonas iv. Spirogyra
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(positive). The catalase test result indicated that my unknown was negative because no bubbles formed when I placed a loop of the organism into hydrogen peroxide. The next step was to examine a blood agar plate to examine the colonial morphology of hemolysis ‚ my organism produced gamma hemolysis on the blood agar because there was no hemolysis on the plate after 48 hours incubation period. After examining my unknown for hemolysis I set a series of five experiments: Bacitracin SX CAMP Enterococcosel
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water because without anything to help it‚ water cannot do anything. For example‚ if you wash your hands and you do not use any soap‚ none of the germs on your hand will be cleaned. Materials: The materials we used in this lab were a petri dish with agar‚ forceps‚ water‚ alcohol‚ hydrogen peroxide‚ antibacterial soap‚ filter paper disc‚ tape‚ and a permanent marker. Procedure: We started this lab on May 12‚ 2014. Using a permanent marker on the bottom of the dish‚ we divided it into 4 sections.
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disk‚ impregnated with the compound to be tested‚ is placed on the surface of the agar. The compound diffuses out from the filter paper into the agar. The concentration of the compound will be higher next to the disk‚ and will decrease gradually as distance from the disk increases. If the compound is effective against bacteria at a certain concentration‚ no colonies will grow wherever the concentration in the agar is greater than or equal to that effective concentration. This region is called the
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completed on an agar plate but on one a much larger scale and where techniques can be perfected before the use of agar plates and specimens are used. Because this experiment uses materials that represent those used in the streaking on agar plates the ability to simulate the events that occur when streaking is similar and allows for visualization instantly. In this experiment materials will be gathered that are representative of the tools need to complete the actual experiment of agar plate streaking
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for antifungal activity of bacterial strain Paenibacillus elgii TS33 25. The nutrient agar medium was prepared‚ sterilized at 121ºC‚ poured into the sterilized peteriplates and allowed to solidify under aseptic conditions. After solidification bacterial strain Paenibacillus elgii TS33 was spot inoculated on nutrient agar medium and incubated at 37ºC for 48 hours. After incubation the molten potato dextrose agar medium containing the spores of test fungus‚ was spread on the same plate and reincubated
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on bacterial growth. By observing the effects of 5 different inhibitors including alcohol‚ bleach‚ soap solution and distilled water‚ it was determined what antiseptic or disinfectant was able to best inhibit this kind of bacterial growth. Nutrient Agar was poured into a Petri dish with four quadrants and then a pipette was used to place bacterial culture on top. Using forceps‚ a filter disc was dipped into each inhibitor and then into a separate quadrant of the Petri dish. The lid of the Petri dish
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LOVELY PROFESSIONAL UNIVERSITY CAPSTONE PROJECT REPORT TOPIC- ANTIMICROBIAL ACTIVITY OF DIFFERENT TYPES OF HONEY. PROJECT GUIDE- SUBMITTED BY- DR. AKSHAY GARG MOHIT KUMAR DEPT. OF BIOTECHNOLOGY REG. NO.- 10800037 ROLL NO- RB1R07B02 B.TECH BIOTECH.(8th sem.)
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INTRODUCTION Total Viable Count is a quantitative idea about the presence of microorganisms such as bacteria‚ yeast and mold in a sample. It counts the number of colonies produced by a very dilute suspension of bacteria on an agar plate and to observe the differential staining behaviour of the living bacteria. This involves counting the colonies produced by viable cells under favourable growth conditions. Some techniques needed before the viable count‚ like pour plate method‚ spread plate method
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