Microbiology Module 02 Homework Assignment Use the information presented in this module along with additional outside research to answer the questions: 1) Compare and contrast prokaryotic and eukaryotic. a) Prokaryotes and eukaryotes are two types of cells that are very different but share some certain properties such as methods of reproduction‚ protein synthesis‚ an organized metabolism‚ response to stimuli‚ and plasma membranes. One significant difference is that prokaryotes are without a cell
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General Purpose media is designed to grow most organisms and do not contain growth inhibitors. Standard Methods Agar and Blood Agar Bases are examples of general purpose media. Differential Media Differential media contain a component that allow an observable change when a specific chemical reaction takes place. Simmons Citrate Agar is an example of a differential medium. In Simmons Citrate Agar‚ there is a pH indicator that turns from green to blue when citrate is utilized as the sole carbon source
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plate and incubated one under aerobic conditions at 37°C and the other under anaerobic conditions at 37°C. Next‚ I used Columbia colistin nalidixic acid agar (Columbia CNA) with 5% sheep blood as my medium. I used the same streak plate technique on this medium as I used on the TSA plates and incubated it at 37°C as well. Then‚ I used MacConkey agar as a medium to detect lactose fermentation‚ used the same streak plate technique with my bacteria‚ and incubated the plate at
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Staphylococcus aureus‚ were grown singly and mixed on four different types of agar in order to observe the varying morphologies within the colonies. Resulting data was analyzed to provide understanding of the use of differing culture media and conditions for bacterial growth. RESULTSFour different agar types were used in this experiment. The first (Nutrient) allowed for growth of both E. coli and S. aureus. The second agar used (MKL) inhibited the growth of S. aureus but allowed the growth of E. coli
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type of appearance with rod-shaped bacteria in general‚ whether gram-positive or gram-negative.] Perform catalase test. {Catalase test is positive.} Inoculate test tubes prepared with the following mediums – Triple Sugar Iron agar slant (TSI slant)‚ Bile Esculin Agar slant (BEA tube)‚ a methyl-red Voges-Proskauer tube (MR-VP tube) and a Urease tube. Incubate the inoculated tubes‚ to be read at the following lab session. Observe results of tests inoculated during the last session [Note: I used
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results of my unknown and Salmonella typhimurium found in the Bergey’s manual. Gram staining showed gram negative rods‚ a motility test was also conducted to see if the bacterium moved or not‚ it was found to be none motile. Three different types of agar plates were used‚ they had two known bacterium put on along with the unknown to be able to compare negative and positive results if the known with the results of the unknown‚ refer to Barbaro (2016) for how the test were done. Table 1: Results of
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source. Simmon’s citrate agar will be prepared by using Oxoid well-prepared Simmon’s citrate agar powder. The Simmon’s citrate agar was prepared by using Oxoid well-prepared Simmon’s citrate agar powder. The preparation was began by suspending 5.75g of the powder in 250 ml of distilled water and mixed thoroughly. The following shows the amount of each composition in 1 litre of Simmon’s citrate agar solution prepared using Simmon’s citrate agar powder. This Simmon’s citrate agar solution was then heated
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and Gram-positive bacteria were observed in the unknown mixed culture (Table 1 and Table 2; Kellenberger‚ 2001). In order to isolate the two different bacteria‚ colonies that grew on the MSA were used to inoculate Gram-positive tests‚ where as MacConkey Agar colonies were used to inoculate Gram-negative tests. Once the colonies were isolated and the appropriate Gram-negative and Gram-positive tests were conducted‚ the identification of both unknown organism were fairly easy. The results from
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Identification of Bacterial Pathogens basic skills in diagnostic bacteriology Dr.T.V.Rao MD Dr.T.V.Rao MD 1 Identification of Microorganisms • For many students and professionals the most pressing topic in microbiology is how to identify unknown specimens. • Why is this important? • Labs can grow‚ isolate and identify most routinely encountered bacteria within 48 hrs of sampling. • The methods microbiologist use fall into three categories: ♣Phenotypic- morphology (micro and
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Blood agar plate This test is used to detect the hemolytic activity in the bacteria. A darkish green color on the media around the bacteria would represent incomplete hemolysis. A transparent media around the bacteria colony represents complete lysis of the red blood cells. If no change is observed around the bacteria colony then the bacteria is non-hemolytic. For my bacteria no change is observed in the media therefore the bacteria is non-hemolytic and negative result. MacConkey Agar test
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