by the high amount of colonies observed on Horse Blood Agar (>108/L)‚ mild amount of protein (30 mg/mL) and very high amount of red blood cells (Large+++) found in the urine. The bacterium identified in this specimen is Pseudomonas aeruginosa. This is confirmed by involving many tests and observation‚ such as Gram stain‚ oxidase test‚ catalase test and the observation of colony morphology on Horse Blood Agar‚ MacConkey agar and Tryptic Soy Agar. Pseudomonas aeruginosa is an opportunistic pathogen‚
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Background: In Jane Horack’s article “Staphylococcus epidermidis”‚ S. epidermidis is described as “gram-positive cocci bacteria that are part of the normal flora on the skin and nasal passages.” The article goes on to say that the species was originally named Staphylococcus Albus by microbiologist Rosenback in 1884. When viewed under a microscope S. epidermidis will appear in chains‚ pairs‚ or grape-like clusters (Horak 1). Taxonomically‚ the species S. epidermidis falls in the genus Staphylococcus
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specific enzymatic reactions or metabolic pathways‚ each was used in a way to help recognize those specifics and identify the unknown cultures. The differential tests used to identify the unknown cultures were Gram stain‚ Catalase‚ Mannitol Salt Agar (MSA)‚ Blood Agar‚ Novobiocin‚ Coagulase‚ and DNAse (Alachi‚ 2007). Rebekah Worley February 21‚ 2012 Mitchell Section 4 Biol 311 Staining and Identifying Unknown Bacteria Introduction: The microbiology lab up to this point has been used to teach the
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2 were Pseudomonas aeruginosa. One of each was used to confirm. Finally only MTCC cultures were used to prepare inoculum. Inoculums Preparation Primary isolation was initiated by nutrient agar plating and subsequently transferred to selective media like Pseudomonas Isolation Agar‚ MacConkey Agar and EMB agar for better isolation. Using sterile inoculation loop colonies of the test organism are transferred to 5ml of sterile nutrient broth and incubated at 37 0C overnight for 24hrs. Then this bacterial
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experiment. The first one I will be talking about is the Eosin Methylene Blue agar or EMB for short. EMB is a selective and differential medium that inhibits the growth of gram-positive‚ but detects and isolate gram-negative enteric bacteria. Mythelene blue inhibits gram-positive. The selective part is used for inhibiting gram-positive and has methylene blue. The differential part is what ferments lactose. MacConkey agar is a selective and differential media used to isolate gram-negative bacteria
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3-2-3-1-Conventional Culture Method (Marks 1972): The infected lesions of the tuberculin positive reactors were collected and placed in to a sterile mortar containing sterile sand. The fat was removed and the suspected parts were cut into minute pieces. Then 2 ml of distilled water added to the mixture‚ homogenized and crushed well until the suspension was obtained. Then 2 ml of H2So4 acid 4% added to the mixture then incubated the mixture at 37 0C for 1/2 h. The mixture was diluted with
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Margaret E Gibson July 20‚ 2009 Microbiology Dr. Metera Lab Report 3: Labs 7 and 8- Metabolism and Biochemical Tests Abstract This experiment focused on metabolism and biochemical tests. The goal of performing these tests was to differentiate microbes from one another and to compare how metabolic and biochemical processes differ from species to species. The tests performed include: the Fermentation of Sugars Test (sucrose‚ glucose‚ and lactose)‚ the Urease Test‚ the Fermentation
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Proteus Mirabilis Proteus mirabilis is part of the Enterobacteriaceae family. It is a small gram-negative Rod‚ and a facultative anaerobe‚ lastly‚ it is a Prokaryote. Furthermore‚ Proteus mirabilis is characterized by its swarming motility‚ its ability to ferment maltose‚ and its inability to ferment lactose. P. mirabilis has the ability to elongate itself and secrete a polysaccharide when in contact with solid surfaces‚ making it extremely motile on items such as medical equipment (catheters)
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ANTIMICROBIAL ACTIVITY OF SOIL USED IN THE TREATMENT OF BACTERIAL DIARRHOEABYSITUMBEKO LIWELEYA(s213459531)Research Proposal Submitted in fulfilment of the requirements for the degree of BTECHBIOMEDICAL TECHNOLOGY | | FACULTY OF HEALTH SCIENCES | atNelson Mandela Metropolitan University | MODULE- RESEARCH METHODOLOGYDUE DATE- 16th MAY‚ 2013.SUPERVISOR- PROFESSOR N. SMITH | | Contents ANTIMICROBIAL PROPERTIES OF SOIL USED IN THE TREATMENT OF BACTERIAL DIARRHOEA
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Salmonella Enterica Lindsy Shellhamer Kaplan University Salmonella Enterica There is a lot to know about this bacterium. There are over 2‚500 different serotypes of Salmonella Enterica‚ for example serotype Typhimurium (Pg‚ 355) (Pommerville‚ 2011). Salmonella Enterica can cause many different infections like salmonellosis‚ typhoid fever‚ and gastroenteritis. Because of carelessness in personal hygiene‚ this kind of infection can turn into an outbreak very easily
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