following microbiological analyses were performed: (i) determination of total aerobic counts on nutrient agar (Oxoid) at 30°C for 48 h‚ (ii) coliforms on MacConkey agar (Oxoid) by incubation at 30°C for 24 h‚ (iii) yeasts and molds by incubation on malt agar (Oxoid) (pH adjusted to 4.5 by sterile solution of 10% lactic acid) at 30°C for 48 h‚ and (iv) staphylococci by incubation on Staphylococcus agar No. 110 medium (Oxoid) at 37°C for 48h [Atlas‚
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Microbiology Study Guide Chapters 1-6 Chapter 1 Main Themes of Microbiology Microorganisms Most are not Pathogens Prokaryotic-no nucleus or organelles Very simplistic—like bacteria Eukaryotic-has nucleus and organelles like mitochondria Includes fungus‚ and animals Acellular-not even a complete cell Includes viruses and prions like those that cause Mad Cow disease Six main types of microorganisms Bacterium (prokaryotic) Fungus (eukaryotic) Algae (eukaryotic) Virus (acellular)
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Bacterial colonies that have a different shape‚ size‚ or color are generally different bacteria. In this exercise you are streaking only one bacterium‚ so all the colonies should look pretty much the same. You will need the following: 2 tryptic soy agar (TSA) plates Broth culture of Escherichia coli After you have streaked your plates‚ they will be incubated at 37°C. The plates MUST be labeled and incubated in an inverted position. Next
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1. Procedure #1 detected 50/100 true positives and 100/100 true negatives. Procedure #2 detected 80/100 true positives and 70/100 true negatives. b. procedure 2 is more sensitive 2. TP/TP+FN = ? b. specificity 3. What might the following indicate? urine: RBCs‚ WBCs‚ nitrite‚ bacteria a. pyelonephritis 4. Why is albumin the first protein to be detected in tests for renal failure? b. its molecular size is smallest 5. Cortisol excess will result in _____ a. hypernatremia c. elevated glucose
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Harley−Prescott: Laboratory Exercises in Microbiology‚ Fifth Edition Front Matter Preface © The McGraw−Hill Companies‚ 2002 PREFACE Take interest‚ I implore you‚ in those sacred dwellings which one designates by the expressive term: laboratories. Demand that they be multiplied‚ that they be adorned. These are the temples of the future—temples of well-being and of happiness. There it is that humanity grows greater‚ stronger‚ better. Louis Pasteur (French chemist‚ founder of microbiology
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A TERM PAPER ON MICROBIOLOGICAL QUALITY CONTROL OF BEER IN BREWING INDUSTRY COMPILED BY IMEVBORE GRACE OLUWATOSIN ACU/570 SUBMITTED TO MISS F. T. OJO OF THE DEPARTMENT OF BIOLOGICAL SCIENCES‚ AJAYI CROWTHER UNIVERSITY‚ OYO‚ OYO STATE. COURSE CODE: MCB 4204 COURSE TITLE: MICROBIOLOGICAL QUALITY ASSURANCE MAY‚ 2010 1 CHAPTER ONE INTRODUCTION 1.0 History of Brewing The art of brewing is as old as civilization. Between 10‚000 and 15‚000 years ago‚ some humans discontinued their nomadic hunting
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INTRODUCTION The name agar originated from the Malay word “Agar-agar”‚ For the sake of simplicity agar-agar was shortened to just agar and is now accepted universally whether in the food and other industries. The use of agar became widespread in Indonesia‚ Malaysia‚ China‚ and Korea‚ where people called it Agar-Agar‚ which means seaweed. Its introduction to Europe dates from 1859‚ almost two hundred years later. Agar has been used in food preparations for over three hundred years. Agar-agar is also practiced
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diffusion rate in agar cubes? Hypothesis: The rate of diffusion in directly related to the surface area to volume ratio of cells and is responsible for the efficiency of absorbing nutrients‚ oxygen‚ minerals etc. in the cell. This ratio is specific to cells as they require a ratio that isn’t big enough to take too long to receive the nutrients and oxygen or too small to impair diffusion‚ known as the maximum sustainable cell size. Due to this it would be expected that the smaller the agar cube the larger
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when the agar blocks are placed in solutions of different concentrations of HCl. As the concentration of HCl increases‚ the rate of diffusion will also increase due to the steeper concentration gradient created. To investigate the change in rate of diffusion when different concentrations of HCl are used‚ I used agar blocks that are stained with universal indicator that would change from green to pink when exposed to HCl. To prepare the agar blocks‚ I used knife and ruler to acquire 5 agar blocks with
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plates. This indicates that the Tn10 exposure was the cause of mutant variation. There were ten hypothesized auxotrophic colonies based on the comparison of mutagenized colonies in minimal and nutrient agar. These ten colonies were confirmed to be auxotrophic by their lack of growth in the minimal agar replica plate. The required growth factors for each auxotroph were determined based on which pool plates fostered growth for each streak. Auxotrophs 1‚ 2‚ 3‚ and 4 require valine as a growth factor
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