distinguish the species of bacteria into two groups Gram-positive and Gram-negative based on the physicochemical properties of the cells. First‚ a smear was prepared by use a sterile transfer loop that been flamed to removes some bacteria from slant agar and placed on the slide; mixed with one drop of water and let air dried. After dried‚ heat fixation the slide by passed the slide over a flame quickly 2-3 times to stick bacteria to the slide. Next‚ the smear was sequence covered with crystal violet
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Question: What antibiotics work best in preventing E. coli k12 from growing; amoxicillin‚ ampicillin‚ or ciprofloxacin? Hypothesis: If ciprofloxacin‚ an antibiotic‚ is added to petri dishes covered in live bacteria and left to sit for four to six days in an incubator at 37 degrees Celsius‚ then the petri dishes containing ciprofloxacin will have the largest zone of inhibition out of all of the other antibiotics tested because research shows that ciprofloxacin in one of the strongest antibiotic currently
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urease. Casein hydrolysis tests for organisms capable of hydrolyzing casein via the casease. Materials and Methods Bile Esculin Hydrolysis The organisms Lactococcus lactis and Enterococcus faecalis were spot-inoculated on a bile esculin agar plate. The bile esculin agar plate is a both selective and differential medium contains primarily esculin. The plate was then inverted and incubated at 37 oC for 24 hours. Bile salts‚ the selective agent‚ can allow only Enterococcus and group d streptococcus to hydrolyse
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is believed that higher temperatures will assist in the growth rate in the agar plates. Therefore it is believed that the agar plates placed in full light will produce more bacteria. Due to the type of light used for the full light part of the experiment there will be higher temperatures and therefore grow better than the no light and day light plates which are at lower temperatures. Equipment and Materials: 1 6 agar plates 2 E. coli and S. albus bacteria 3 Light 4 Day light 5 Cupboard
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cotton swabs are first made damp using sterilized water. The swabs are rubbed in the following 5 places and then rolled and rubbed in the petri dishes: A: Rub the swab on the pass-code for the main entrance of the school and then roll it over the agar in petri dish A. B: Rub the swab on the door handle in room 3.1 and then
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Apparatus: • Agar Powder • Distilled Water • McCartney Bottles • Brassica Seeds • Scissors Diagram of Apparatus: Method: • Place some Rapid cycling Brassica seeds onto a damp sponge placed in a plastic tray. Cover with cling film and place in a warm‚ light place to germinate. • When the seeds have germinated‚ they are ready to culture. • Measure out 2.5 g of agar powder and add to 250 cm3 of distilled water. Heat and stir gently until the agar dissolves
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the centre of the cells further away from the surface. Therefore‚ when dipped in sodium hydroxide‚ the larger cells will not be dyed pink all the way through like the smaller cells after the ten minutes. Materials: 1x1x1 cm agar cube 2x2x2 cm agar cube 3x3x3 cm agar cube 100 ml of sodium hydroxide Goggles Petri dish Scissors Timing device Tweezers Beaker Graduated cylinder Procedure: Refer to ’Nelson Science Perspectives 10’ pages 38-39‚ section 2.4; ’What Limits Cell Size?’.
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(Salivary amylase) against temperature” aims to know and observe the enzyme activity of the human saliva. The research only included the use of starch-agar as the medium to observe enzyme activity during the experiment. Five starch-agar plates were prepared and each were labeled 1‚ 2‚ 3‚ 4 and 5 respectively. One mL of saliva were placed in each starch-agar plate which was holed then incubated for 24 hours. The plate labeled 1 was stored with 0ºC. The plate numbered 2 was stored at 15ºC‚ plate 3 at
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POLYMER SYNTHESIS AND PROPERTIES AIM: The aim of the experiment was to prepare three different polymers‚ namely nylon 66‚ Agar Gel and slime‚ being able to differentiate between the configuration and analysis among the three structures‚ noting the physical characteristics of each polymer and the chemical reactions that occur during the formation of the polymer. Pre- lab questions 1. Is Nylon 66 a step or chain – growth polymer? Define both types of polymerization in your answer. Nylon 66
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aeruginosa was gram-positive and therefore it would be susceptible. That was my hypothesis.We divided antiseptic agar plate into quadrants and divided antibiotics agar plate into six areas. We wrote the organisms’ name‚ date‚ and the temperature on the bottom of the agar plates. For antiseptic‚ our group used one color code for each quadrant. We streaked the swab in three directions on the agar plate and used sterilize forceps to remove filter paper discs from the container‚ and dipped it into solution
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