Molecular Biology Lab Report Payton Jackson Introduction In this lab‚ I am going to use antibiotic-resistance plasmids to transform Escherichia coli. Materials For this lab you will need the following: LB Agar Petri dishes Beakers Test tubes CaCl2 solution Sensitive E. coli (-ampR) amp plasmids ampicillin -amp cells Water bath to heat shock cells A freezer to incubate cells Process Step 1: Wash hands and sanitize lab setting. This will prevent anything reacting with a
Premium Bacteria Escherichia coli DNA
The first test conducted on unknown bacteria 32 was the Gram stain. From this stain‚ unknown 32 was found to be a Gram-positive cocci. This test eliminated all possible Gram-negative bacteria‚ Gram-positive rods and Gram-positive spirillium. Next‚ the endospore test determined whether or not the Gram-positive bacteria contained endospores. With the use of malachite green‚ steam‚ and safranin it was found that unknown bacteria 32 did not contain endospores. This eliminated Gram-positive cocci Sporosarcina
Premium Bacteria Microbiology Staining
synthesis of ATP (Hertfordshire‚ 2011). Without ATP cellular functions fail and then die. Research Question: How does increasing the concentrations of Dettol Antiseptic Liquid from 0% to 100% affect the growth of Escherichia coli bacteria on the agar plates? Hypothesis: As the concentration of Dettol Antiseptic Liquid increases the inhibition of bacterial growth around the antiseptic disks also increases. Variables: |Type of variable
Premium Bacteria Immune system Inflammation
Medical Mycology: Yeast and Pneumocystis| Reading Assignment:|Mahon‚ Chapter 10‚ pgs 215-219‚ Chapter 27‚ pgs 626-629‚ 634-636‚ Appendix B Lecture Notes: Medical Mycology| |U of W Tutorial on Mycology (organisms listed in objectives)‚ www.medtraining.org[->0]| _____________________________________________________________________ 1. Discuss the difference between yeasts and molds. Fungi seen in the clinical laboratory can be generally separated into two groups based on the appearance of the
Premium Yeast Fungus Candida albicans
| conc. H2SO4 | |glucose‚ fructose‚ maltose‚ sucrose‚ lactose‚ |Molisch reagent | |agar-agar‚ gum arabic‚glycogen‚ cotton‚ |I2 in KI solution (Lugol’s iodine reagent) | |starch |Benedict’s reagent
Premium Glucose Iodine Carbohydrate
pyogenes. The sputum sample was first inoculated in the agar plates MAC‚ SBAP‚ CHOC and also a direct gram stain of the sample was performed. After a day of incubation‚ the MAC agar gave a negative result‚ therefore it is not a Gram-negative bacteria and non-lactose fermenting. The SBAP and CHOC showed growth of colonies. The CHOC agar had a mixed formation of colonies (gray colonies and 2+ tan colonies)‚ so it was isolated in another CHOC agar and it was mixed again. A gram stain procedure was done
Premium Bacteria Microbiology Staining
dish of nutrient agar‚kept led on ‚and used the marker to divided the petri dish ‚into teo sector. -opened lte lid slightly‚inoculate sector number 1 with thumbprint. -Taked sterial wire loop to transferred colony from a broth to an agar plate. -The loop should be cooled in the air for 10 to 20 sec and placed on the line drawed. -Remove a sample from a broth culture by using a sterile wire loop. -Transferred the sample from borth to agar plat. - When inoculated an agar plate‚ drawed the
Premium Bacteria Microbiology Microorganism
I removed he swabs from their packets and swabbed the inside of my cheek and put them back in their packets. 3. I then lifted the lid of the TSA plate slightly at an angle to prevent airborne microorganisms from contaminating the agar and smeared the surface of the agar with the swab from the floor and labelled it ‘TSA floor’. I did the same for the swab from my cheek and labelled it ‘TSA cheek’. I repeated this process for the SDA
Premium Bacteria Microbiology Microorganism
shigellosis include Btilliant Green Agar‚ and Triple Sugar-Iron Agar. Expected results in a confirmed case of shigellosis are as follows: Brilliant Green Agar – Isolated Shigella colonies which do not ferment lactose or sucrose and appear red or white in color with no growth to trace growth on the Agar plate will be present. Triple Sugar-Iron Agar – Presence of Shigella will manifest as a red slant with a yellow butt with no H2S present. In Brilliant Green Agar‚ E. coli O157 would present as isolated
Premium Algae Algal bloom Bacteria
temperature as temperature is lower their growth is also slower and temperature above 30ºC may cause mutation in petite and sporulation is highly inhibited. 6. Isolating Single Colonies: Done by streaking cultures out on an agar plate to dilute the cells on the surface of the agar‚ overlapping streaks‚ separation or distribution of cells to form isolated single colonies. 7. Estimating the Number of Yeast Cells in a Culture: How to differentiate between molds and yeast culture: Traditional methods:
Premium Bacteria Microorganism Yeast